LOCUS       sequence-83730-e         956 bp    DNA     linear   UNA 15-APR-2025
DEFINITION  chromosomal tagging with stable GFP.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 956)
  AUTHORS   Ko N, Nishihama R, Tully GH, Ostapenko D, Solomon MJ, Morgan DO, 
            Pringle JR
  TITLE     Identification of yeast IQGAP (Iqg1p) as an 
            anaphase-promoting-complex substrate and its role in 
            actomyosin-ring-independent cytokinesis.
  JOURNAL   Mol Biol Cell. 2007 Dec;18(12):5139-53. Epub 2007 Oct 17.
  PUBMED    17942599
REFERENCE   2  (bases 1 to 956)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Biol 
            Cell."; date: "2007-12"; volume: "18(12)"; pages: "5139-53. Epub 
            2007 Oct 17"
COMMENT     Sequence Label: pFA6a-GFP (F64L, S65T, V163A)-TRP1
FEATURES             Location/Qualifiers
     source          1..956
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             40..750
                     /codon_start=1
                     /product="S65T variant of Aequorea victoria green 
                     fluorescent protein (Heim et al., 1995)"
                     /label=GFP (S65T)
                     /note="excitable with blue light"
                     /translation="SKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKF
                     ICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDGN
                     YKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKAN
                     FKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLEF
                     VTAAGITHGMDELYK"
     primer_bind     complement(67..95)
                     /label=GFP-R
                     /note="GFP, reverse primer. Does NOT anneal to EGFP"
     primer_bind     690..710
                     /label=GFP-F
                     /note="GFP, forward primer. Does NOT anneal to EGFP"
     terminator      774..939
                     /gene="S. cerevisiae ADH1"
                     /label=ADH1 terminator
                     /note="transcription terminator for the S. cerevisiae 
                     alcohol dehydrogenase 1 (ADH1) gene"
     primer_bind     complement(829..853)
                     /label=pHybLex-R
                     /note="ADH terminator, reverse primer"
ORIGIN
        1 tcgtacgctg caggtcgacg gatccccggg ttaattaaca gtaaaggaga agaacttttc
       61 actggagttg tcccaattct tgttgaatta gatggtgatg ttaatgggca caaattttct
      121 gtcagtggag agggtgaagg tgatgcaaca tacggaaaac ttacccttaa atttatttgc
      181 actactggaa aactacctgt tccatggcca acacttgtca ctactctcac ttatggtgtt
      241 caatgctttt caagataccc agatcacatg aaacagcatg actttttcaa gagtgccatg
      301 cccgaaggtt atgtacagga aagaactata tttttcaaag atgacgggaa ctacaagaca
      361 cgtgctgaag tcaagtttga aggtgatacc cttgttaata gaatcgagtt aaaaggtatt
      421 gattttaaag aagatggaaa cattcttggg cacaaattgg aatacaacta taactcacac
      481 aatgtataca tcatggcaga caaacaaaag aatggaatca aagctaactt caaaattaga
      541 cacaacattg aagatggaag cgttcaacta gcagaccatt atcaacaaaa tactccaatt
      601 ggcgatggcc ctgtcctttt accagacaac cattacctgt ccacacaatc tgccctttcg
      661 aaagatccca acgaaaagag agaccacatg gtccttcttg agtttgtaac agctgctggg
      721 attacacatg gcatggatga actatacaaa tagggcgcgc cacttctaaa taagcgaatt
      781 tcttatgatt tatgattttt attattaaat aagttataaa aaaaataagt gtatacaaat
      841 tttaaagtga ctcttaggtt ttaaaacgaa aattcttatt cttgagtaac tctttcctgt
      901 aggtcaggtt gctttctcag gtatagtatg aggtcgctct tattgaccac acctct
//
