LOCUS       sequence-83761-e        1289 bp    DNA     linear   UNA 15-APR-2025
DEFINITION  bacterial expression plasmid.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1289)
  TITLE     SGC_OxfordQ1Q2_2014
REFERENCE   2  (bases 1 to 1289)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: PRKCBP1A-c092
FEATURES             Location/Qualifiers
     source          1..1289
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             4..21
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             31..201
                     /codon_start=1
                     /product="Z domain of staphylococcal protein A, engineered 
                     to contain multiple positive charges (Hedhammar and Hober, 
                     2007)"
                     /label=Z-basic tag
                     /note="useful for purifying tagged proteins by 
                     cation-exchange chromatography"
                     /translation="VDNKFNKERRRARREIRHLPNLNREQRRAFIRSLRDDPSQSANLL
                     AEAKKLNDAQPK"
     CDS             208..228
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
     CDS             1269..1286
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
ORIGIN
        1 atgcaccatc atcatcatca ttcttctggt gtggataaca agttcaacaa ggagcgtcga
       61 agagctcgcc gtgaaattcg ccatctgccg aacctgaacc gcgaacagcg tcgcgcattt
      121 attcgcagcc tgcgcgatga tccgagccag agcgcgaacc tgctggcgga agcgaagaag
      181 ctgaacgatg cgcagccgaa gggtaccgag aacctgtact tccaatccat gcaggatgga
      241 cggaatgatt tctactgctg ggtttgtcac cgggaaggcc aagtcctttg ctgtgagctc
      301 tgtccccggg tttatcacgc taagtgtctg agactgacat cggaaccaga gggggactgg
      361 ttttgtcctg aatgtgagaa aattacagta gcagaatgca tcgagaccca gagtaaagcc
      421 atgacaatgc tcaccattga acagttatcc tacctgctca agtttgccat tcagaaaatg
      481 aaacagccag ggacagatgc attccagaag cccgttccat tggaacagca ccctgactat
      541 gcggaataca tcttccatcc aatggacctt tgtacattgg aaaagaatgc gaaaaagaaa
      601 atgtatggct gcacagaagc cttcctggct gatgcaaagt ggattttgca caactgcatc
      661 atttataatg ggggaaatca caaattgacg caaatagcga aagtagtcat caaaatctgt
      721 gaacatgaga tgaatgaaat cgaagtatgt ccagaatgtt atctagctgc ttgccaaaaa
      781 cgagataact ggttttgtga gccttgtagc aatccacatc ctttggtctg ggccaaactg
      841 aaggggtttc cattctggcc tgcaaaagct ctaagggata aagacgggca ggtcgatgcc
      901 cgattctttg gacaacatga cagggcctgg gttccaataa ataattgcta cctcatgtct
      961 aaagaaattc ctttttctgt gaaaaagact aagagcatct tcaacagtgc catgcaagag
     1021 atggaggttt acgtggagaa catccgcagg aagtttgggg tttttaatta ctctccattt
     1081 aggacaccct acacacccaa cagccagtat caaatgctgc tcgatcccac caaccccagc
     1141 gccggcactg ccaagataga caagcaggag aaggtcaagc tcaactttga catgacggca
     1201 tcctgacagt aaaggtggat acggatccga attcgagctc cgtcgacaag cttgcggccg
     1261 cactcgagca ccaccaccac caccactga
//
