LOCUS       sequence-83786-e        1279 bp    DNA     linear   UNA 15-APR-2025
DEFINITION  bacterial expression plasmid.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1279)
  TITLE     SGC_OxfordQ3Q4_2013
REFERENCE   2  (bases 1 to 1279)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: DYRK2A-c023
FEATURES             Location/Qualifiers
     source          1..1279
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             4..21
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             46..66
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
ORIGIN
        1 atgcaccatc atcatcatca ttcttctggt gtagatctgg gtaccgagaa cctgtacttc
       61 caatccatgg ggaaggtgaa agccaccccc atgacacctg aacaagcaat gaagcaatac
      121 atgcaaaaac tcacagcctt cgaacaccat gagattttca gctaccctga aatatatttc
      181 ttgggtctaa atgctaagaa gcgccagggc atgacaggtg ggcccaacaa tggtggctat
      241 gatgatgacc agggatcata tgtgcaggtg ccccacgatc acgtggctta caggtatgag
      301 gtcctcaagg tcattgggaa ggggagcttt gggcaggtgg tcaaggccta cgatcacaaa
      361 gtccaccagc acgtggccct aaagatggtg cggaatgaga agcgcttcca ccggcaagca
      421 gcggaggaga tccgaatcct ggaacacctg cggaagcagg acaaggataa cacaatgaat
      481 gtcatccata tgctggagaa tttcaccttc cgcaaccaca tctgcatgac gtttgagctg
      541 ctgagcatga acctctatga gctcatcaag aagaataaat tccagggctt cagtctgcct
      601 ttggttcgca agtttgccca ctcgattctg cagtgcttgg atgctttgca caaaaacaga
      661 ataattcact gtgaccttaa gcccgagaac attttgttaa agcagcaggg tagaagcggt
      721 attaaagtaa ttgattttgg ctccagttgt tacgagcatc agcgtgtcta cacgtacatc
      781 cagtcgcgtt tttaccgggc tccagaagtg atccttgggg ccaggtatgg catgcccatt
      841 gatatgtgga gcctgggctg cattttagca gagctcctga cgggttaccc cctcttgcct
      901 ggggaagatg aaggggacca gctggcctgt atgattgaac tgttgggcat gccctcacag
      961 aaactgctgg atgcatccaa acgagccaaa aattttgtga gctccaaggg ttatccccgt
     1021 tactgcactg tcacgactct ctcagatggc tctgtggtcc taaacggagg ccgttcccgg
     1081 agggggaaac tgaggggccc accggagagc agagagtggg ggaacgcgct gaaggggtgt
     1141 gatgatcccc ttttccttga cttcttaaaa cagtgtttag agtgggatcc tgcagtgcgc
     1201 atgaccccag gccaggcttt gcggcacccc tggctgagga ggcggttgcc atgacagtaa
     1261 aggtggatac ggatccgaa
//
