LOCUS       sequence-83799-e        1173 bp    DNA     linear   UNA 15-APR-2025
DEFINITION  bacterial expression plasmid.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1173)
  TITLE     SGC_OxfordQ3Q4_2013
REFERENCE   2  (bases 1 to 1173)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: CUL3A-c013
FEATURES             Location/Qualifiers
     source          1..1173
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             1102..1122
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
     CDS             1123..1140
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             1141..1164
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
ORIGIN
        1 atgactatgg acgaaaaata cgtcaattcg atttgggatc tgctgaagaa cgctatccag
       61 gagattcagc gtaaaaacaa ttctggtctg tcatttgaag agctgtaccg caacgcctat
      121 actatggtgc tgcacaagca tggtgaaaaa ctgtataccg gcctgcgtga agtggtcacc
      181 gagcacctga tcaataaggt gcgcgaggat gtcctgaaca gcctgaataa taacttcctg
      241 cagaccctga atcaggcctg gaacgaccat cagaccgcta tggtgatgat ccgtgacatt
      301 ctgatgtaca tggatcgcgt ttatgtgcag cagaataacg tcgaaaatgt ttacaacctg
      361 ggtctgatca tttttcgtga ccaggttgtg cgctatggct gcatccgtga ccacctgcgc
      421 cagaccctgc tggatatgat tgcccgtgaa cgcaagggcg aggtcgttga tcgtggcgcc
      481 atccgcaacg cttgtcagat gctgatgatt ctgggtctgg aaggccgttc tgtctacgaa
      541 gaggacttcg aggcgccctt tctggaaatg agtgcagagt tctttcagat ggaatcgcag
      601 aaattcctgg cggaaaacag cgcatcagtt tatatcaaga aagtggaagc tcgtatcaac
      661 gaagagattg agcgcgttat gcattgcctg gataagtcaa ccgaagagcc aattgtgaaa
      721 gtggtcgaac gcgagctgat ctctaagcac atgaaaacca ttgtcgaaat ggaaaacagc
      781 ggcctggttc atatgctgaa gaacggtaaa accgaagacc tgggctgtat gtacaagctg
      841 ttcagccgtg tgccgaatgg tctgaaaacc atgtgcgaat gtatgagcag ctatctgcgt
      901 gagcagggca aggcgctggt gtccgaagag ggtgaaggca aaaaccctgt ggactaccgc
      961 cagggtctgg acgatctgaa gagccgtttc gaccgctttc tgctggagtc cttcaataac
     1021 gatcgtctgt ttaaacagac cattgcaggc gacttcgaat actttctgaa tctgaacagt
     1081 cgctcgcccg agtatctggc agagaacctc tacttccaat cgcaccatca tcaccaccat
     1141 gattacaagg atgacgacga taagtgagga tcc
//
