LOCUS       sequence-83964-e        1203 bp    DNA     linear   UNA 15-APR-2025
DEFINITION  Luciferase reporter assay for CIP2A with mutated firefly stop codon 
            that also has five miR-375 binding site mutations.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1203)
  AUTHORS   Jung HM, Patel RS, Phillips BL, Wang H, Cohen DM, Reinhold WC, Chang
            LJ, Yang LJ, Chan EK
  TITLE     Tumor suppressor miR-375 regulates MYC expression via repression of 
            CIP2A coding sequence through multiple miRNA-mRNA interactions.
  JOURNAL   Mol Biol Cell. 2013 Jun;24(11):1638-48, S1-7. doi: 
            10.1091/mbc.E12-12-0891. Epub 2013 Apr 3.
  PUBMED    23552692
REFERENCE   2  (bases 1 to 1203)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1091/mbc.E12-12-0891"; journalName: "Mol Biol Cell"; date: 
            "2013-06"; volume: "24"; issue: "11"; pages: "1638-48, S1-7"
COMMENT     Sequence Label: pMiR-CIP2A-stop-mut-miR-375-mutA-E
FEATURES             Location/Qualifiers
     source          1..1203
                     /mol_type="genomic DNA"
                     /organism="unspecified"
ORIGIN
        1 ttaggccaag gtgctaaaga tgacggcaag ttggacgccc gcaagatccg cgagattctc
       61 attaaggcca agaagggcgg aaagatcgcc gtggatccta acaattggca gagctcagaa
      121 ttcggcacga gcggcacgat gagaatatct gacataatgg atgtatatga aatgaaacta
      181 tccacattag cttccaaaga aagcaggcta caagatcttt tggaaacaaa agctctagcc
      241 cttgcacagg ctgatagact gattgctcag catcgctgtc aaagaactca agctgaaaca
      301 gaggcacgga cacttgctag tatgttgaga gaagttgaga gaaaaaatga agagcttagt
      361 gtgttgctga aggcgcattg tgttgaatca gaaagagcgc agagtgatat tgttgttctc
      421 tttcaacata ataggaagtt agagtctgtg gctgaagaac atgaaatact gacaaaatcc
      481 tacatggaac ttcttcagag aaatgaaagt actgaaaaga agaataaaga tttacagatc
      541 acatgtgatt ctctgatgtt acaaattgag acagtgaaaa agttgaatga gtcactcaag
      601 gattgtaatg aaaaaagtat tgcccaatta atagagaaag aagaacagag aaaagaagta
      661 cagaatcagc tagtagacag agaacataag ctagcaaatt tgcatcaaaa aacaaaagta
      721 caagaagaaa agattaaaac cttacaaaag gaaagggaag ataaggaaga aaccattgat
      781 atccttagaa aagaattaag cagaacagaa cagataagaa aagagttgag cattaaggct
      841 tcctccctag aggttcaaaa ggcacaatta gaaggtcgtt tggaagagaa agagtccttg
      901 gtgaaacttc agcaagagga attgattgta cactcccaca tgatagcaat gatccacagt
      961 ttaagtgtgg aaaaataaat ccagaaactg tgaatctcag tatatagaca ttatggcatt
     1021 ttggaatttg taatctcatg atattttttg atgtatttat cttattgagg ggggtggnng
     1081 gggagtaatt tgtgacttcg ttacatagag ttatttatct atttagtaag acccttgatc
     1141 tgtgcatgtt ttnntgnntt gatganttnn nntttctagt tttacctttt ggttcctggc
     1201 cct
//
