LOCUS       sequence-83965-e        1164 bp    DNA     linear   UNA 15-APR-2025
DEFINITION  Luciferase reporter assay for CIP2A with mutated firefly stop codon 
            that also has five miR-375 binding site mutations.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1164)
  AUTHORS   Jung HM, Patel RS, Phillips BL, Wang H, Cohen DM, Reinhold WC, Chang
            LJ, Yang LJ, Chan EK
  TITLE     Tumor suppressor miR-375 regulates MYC expression via repression of 
            CIP2A coding sequence through multiple miRNA-mRNA interactions.
  JOURNAL   Mol Biol Cell. 2013 Jun;24(11):1638-48, S1-7. doi: 
            10.1091/mbc.E12-12-0891. Epub 2013 Apr 3.
  PUBMED    23552692
REFERENCE   2  (bases 1 to 1164)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1091/mbc.E12-12-0891"; journalName: "Mol Biol Cell"; date: 
            "2013-06"; volume: "24"; issue: "11"; pages: "1638-48, S1-7"
COMMENT     Sequence Label: pMiR-CIP2A-stop-mut-miR-375-mutA-E
FEATURES             Location/Qualifiers
     source          1..1164
                     /mol_type="genomic DNA"
                     /organism="unspecified"
ORIGIN
        1 ccgatccgtt ccgagcccac ctttctagag gcggcggtgg aatcgaaatc tcgtgatggc
       61 aggttgggcg tcgcttggtc ggtcattttc ttcgaatcta gatgccgcgg ccgctctaga
      121 agtactctcg agtttttttt tttttttttt actttacttt ttacttttac tctctctata
      181 tttttatttt gtaatcttta ggactgttat accaagaaag tttggtctta agatctaaat
      241 aatcacaaat gagaataaat aagacaggca aaacagaata gaaagcaact aagaaaaagg
      301 aaagaacctt agaaaaatga taatggaggt taaaaatgat tttctaatgt aagaaccaaa
      361 gaaaacctta agccataaaa gccagaaaca aaaagtaaaa cttagaaaat taaatttcta
      421 ttcaaactat caaataaaaa acatgcaaca gatcagggtc tttactaaat tagataataa
      481 cttcttattg ttacgaagtc acaaattaac tcccctaccc accccccctc caatagataa
      541 atacatcaaa aatatcatga gattacaaat tccaaaatgc cataatgtct atatactgag
      601 attcacagtt tctggattta tttttccacc acttaaactg tggatcattg ctatcatgtg
      661 ggagtgtaca atcaattcct cttgctgaag tttcaccaag gactctttct cttccaaacg
      721 accttctaat tgtgcctttt gaacctctag ggaggaagcc ttaatgctca actcttttct
      781 tatctgttct gttctgctta attcttttct aaggatatca atggtttctt ccttatcttc
      841 cctttccttt tgtaaggttt taatcttttc ttcttgtact tttgtttttt gatgcaaatt
      901 tgctagctta tgttctctgt ctactagctg attctgtact tcttttctct gttcttcttt
      961 ctctattaat tgggcaatac ttttttcatt acaatccctt gagtgactca ttcaactttt
     1021 tcactgtctc aatttgtaac atcagagatc acatgtgatc tgtaatctna tcctctnagt
     1081 accttcattt ctctgaggag tcatgtagat ttgtcagtat tnnnnctnag ccagactcta
     1141 acctctatat gngtgggaag gaag
//
