LOCUS       sequence-83966-e        1322 bp    DNA     linear   UNA 14-APR-2025
DEFINITION  Luciferase reporter assay for microRNA binding on HPV16 E7.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1322)
  AUTHORS   Jung HM, Phillips BL, Chan EK
  TITLE     miR-375 activates p21 and suppresses telomerase activity by 
            coordinately regulating HPV E6/E7, E6AP, CIP2A, and 14-3-3zeta.
  JOURNAL   Mol Cancer. 2014 Apr 8;13(1):80. doi: 10.1186/1476-4598-13-80.
  PUBMED    24708873
REFERENCE   2  (bases 1 to 1322)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 14, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol 
            Cancer."; date: "2014-04-8"; volume: "13(1):80. doi"; pages: " 
            10.1186/1476-4598-13-80"
COMMENT     Sequence Label: pMiR-HPV16-E7
FEATURES             Location/Qualifiers
     source          1..1322
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(616..635)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    660..707
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
ORIGIN
        1 tcnagtgtca gaggtgccta aagatgaccg gcagttggac gcccgcaaga tccgcgagat
       61 tctcattaag gccaagaagg gcggaaagat cgccgtgtaa caattggcag agctcagaat
      121 tccatgcatg gagatacacc tacattgcat gaatatatgt tagatttgca accagagaca
      181 actgatctct actgttatga gcaattaaat gacagctcag aggaggagga tgaaatagat
      241 ggtccagctg gacaagcaga accggacaga gcccattaca atattgtaac cttttgttgc
      301 aagtgtgact ctacgcttcg gttgtgcgta caaagcacac acgtagacat tcgtactttg
      361 gaagacctgt taatgggcac actaggaatt gtgtgcccca tctgttctca gaaaccataa
      421 gcggccgcgg catctagatt cgaagaaaat gaccgaccaa gcgacgccca acctgccatc
      481 acgagatttc gattccaccg ccgccttcta tgaaaggttg ggcttcggaa tcgttttccg
      541 ggacgccggc tggatgatcc tccagcgcgg ggatctcatg ctggagttct tcgcccaccc
      601 tagggggagg ctaactgaaa cacggaagga gacaataccg gaaggaaccc gcgctatgac
      661 ggcaataaaa agacagaata aaacgcacgg tgttgggtcg tttgttcata aacgcggggt
      721 tcggtcccag ggctggcact ctgtcgatac cccaccgaga ccccattggg gccaatacgc
      781 ccgcgtttct tccttttccc caccccaccc cccaagttcg ggtgaaggcc cagggctcgc
      841 agccaacgtc ggggcggcag gccctgccat agcctcaggt tactcatata tactttagat
      901 tgatttaaaa cttcattttt aatttaaaag gatctaggtg aagatccttt ttgataatct
      961 catgaccaaa atcccttaac gtgagttttc gttccactga gcgtcagacc ccgtagaaaa
     1021 gatcaaagga tcttcttgag atcctttttt ttctgggggt aatctgctgc ttgcaaacaa
     1081 aaaaaccacc cgctaccagg cggtggttgt ttggccggat cagagctacc aacttctttt
     1141 tccgaaggta actggcttca gcagagcggc agatccaaat acctgttctt ctctatgtga
     1201 gcgtagttag gcacccantt tcaagaacct ttgtagnccg cctacatata cctnggctgc
     1261 tnnctgttna caagtggcng ctgncanngg cgagatannn cgtgtctctt caacacgnnn
     1321 ng
//
