LOCUS       sequence-83979-e         789 bp    DNA     linear   UNA 15-APR-2025
DEFINITION  PLtetO-1:Agn43RBS:mCherry, PLlacO-1:RBS(st7):csrA
             .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 789)
  TITLE     Lim Lab plasmids
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 789)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pHL1928
FEATURES             Location/Qualifiers
     source          1..789
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     terminator      267..353
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     protein_bind    408..424
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     complement(745..762)
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 aggaggcgcc ctgaagggcg agatcaagca gaggctgaag ctgaaggacg gcggccacta
       61 cgacgctgag gtcaagacca cctacaaggc caagaagccc gtgcagctgc ccggcgccta
      121 caacgtcaac atcaagttgg acatcacctc ccacaacgag gactacacca tcgtggaaca
      181 gtacgaacgc gccgagggcc gccactccac cggcggcatg gacgagctgt acaagtaagg
      241 gcccatggta cgcgtgctag aggcatcaaa taaaacgaaa ggctcagtcg aaagactggg
      301 cctttcgttt tatctgttgt ttgtcggtga acgctctcct gagtaggaca aatccgccgc
      361 cctagaccta ggcgcggatc cataaatgtg agcggataac attgacattg tgagcggata
      421 acaagatact cccgggtaag gaggaaaaaa aatgctgatt ctgactcgtc gagttggtga
      481 gaccctcatg attggggatg aggtcaccgt gacagtttta ggggtaaagg gcaaccaggt
      541 acgtattggc gtaaatgccc cgaaggaagt ttctgttcac cgtgaagaga tctaccagcg
      601 tatccaggct gaaaaatccc agcagtccag ttactaaggt acctgaaaga agctttaatc
      661 gtacagggta gtacaaataa aaaaggcacg tcagatgacg tgcctttttt cttgtgagca
      721 gtgaattcgg atatattccg cttcctcgct cactgactcg ctacgctcgg tcgttcgact
      781 gcggcgagc
//
