LOCUS       sequence-84011-e        3639 bp    DNA     circular SYN 14-APR-2025
DEFINITION  cRNA probe to detect the slc6a3 transcript.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3639)
  AUTHORS   Bai Q, Burton EA
  TITLE     Cis-acting elements responsible for dopaminergic neuron-specific 
            expression of zebrafish slc6a3 (dopamine transporter) in vivo are 
            located remote from the transcriptional start site.
  JOURNAL   Neuroscience. 2009 Dec 15;164(3):1138-51. doi: 
            10.1016/j.neuroscience.2009.09.014. Epub 2009 Sep 12.
  PUBMED    19755139
REFERENCE   2  (bases 1 to 3639)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 14, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.neuroscience.2009.09.014"; journalName: "Neuroscience"; 
            date: "2009-12-15- 15"; volume: "164"; issue: "3"; pages: "1138-51"
COMMENT     Sequence Label: slc6a3 probe
FEATURES             Location/Qualifiers
     source          1..3639
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      4..459
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(91..110)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     301..322
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     585..607
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     599..616
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     600..616
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     626..645
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        626..644
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     669..685
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     primer_bind     670..686
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1398..1414)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1398..1414)
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     primer_bind     complement(1451..1471)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(1451..1469)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(1490..1506)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1490..1506)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(1503..1525)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    1514..1530
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(1538..1544,1545..1562,1563..1568))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    1583..1604
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(1721..1738)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(1892..2480)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1972..1991)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(2651..3442,3443..3511))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     3274..3293
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(3512..3616)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 ctgacgcgcc ctgtagcggc gcattaagcg cggcgggtgt ggtggttacg cgcagcgtga
       61 ccgctacact tgccagcgcc ctagcgcccg ctcctttcgc tttcttccct tcctttctcg
      121 ccacgttcgc cggctttccc cgtcaagctc taaatcgggg gctcccttta gggttccgat
      181 ttagtgcttt acggcacctc gaccccaaaa aacttgatta gggtgatggt tcacgtagtg
      241 ggccatcgcc ctgatagacg gtttttcgcc ctttgacgtt ggagtccacg ttctttaata
      301 gtggactctt gttccaaact ggaacaacac tcaaccctat ctcggtctat tcttttgatt
      361 tataagggat tttgccgatt tcggcctatt ggttaaaaaa tgagctgatt taacaaaaat
      421 ttaacgcgaa ttttaacaaa atattaacgc ttacaatttc cattcgccat tcaggctgcg
      481 caactgttgg gaagggcgat cggtgcgggc ctcttcgcta ttacgccagc tggcgaaagg
      541 gggatgtgct gcaaggcgat taagttgggt aacgccaggg ttttcccagt cacgacgttg
      601 taaaacgacg gccagtgagc gcgcgtaata cgactcacta tagggcgaat tgggtaccgg
      661 gccccccctc gaggtcgacg gtatcgataa gcttgatatc gaattctaca tggagctggc
      721 gctgggtcaa tataaccgtg aaggagccgc cggcgtctgg aagatctgcc ccatattcaa
      781 aggcgtgggc ttcacggtga tcctgatctc gctgtatgtg ggcttctact acaacgtgat
      841 catcgcctgg gctctgtttt acctgttctc ctccttctcc ggggagctgc cctggatcca
      901 ctgcaacaac acctggaaca gcccaaactg ctccgacctc aacgccacgc tgctcaacga
      961 cacgtacaag accacgcccg cgctcgagta cttcgagcgg ggtgttctgc atgttcatga
     1021 aagctctggt attgatgatc tgggtgcgcc gcgctggcag ctgaccgcct gtctggccgt
     1081 ggtcatcgtg gtcctctact tcagcctctg gaaaggagtg aagacgtctg ggaaggtggt
     1141 gtggatcact gccacgatgc cgtatgtggt cctgaccgtg ctgctcctgc gtggcgtcac
     1201 tctgcctgga gccatcgacg gcattaaagc ttacctgagt gtggacttcc tgcgcctgta
     1261 tgatgctcag gtctggatcg aggccgccac gcagatctgc ttttctctgg gagtcgggtt
     1321 tggtgtgcta atcgccttct ccagctacaa caaattcagc aacaactgct acagagacga
     1381 attcctgcag cccgggggat ccactagttc tagagcggcc gccaccgcgg tggagctcca
     1441 gcttttgttc cctttagtga gggttaattg cgcgcttggc gtaatcatgg tcatagctgt
     1501 ttcctgtgtg aaattgttat ccgctcacaa ttccacacaa catacgagcc ggaagcataa
     1561 agtgtaaagc ctggggtgcc taatgagtga gctaactcac attaattgcg ttgcgctcac
     1621 tgcccgcttt ccagtcggga aacctgtcgt gccagctgca ttaatgaatc ggccaacgcg
     1681 cggggagagg cggtttgcgt attgggcgct cttccgcttc ctcgctcact gactcgctgc
     1741 gctcggtcgt tcggctgcgg cgagcggtat cagctcactc aaaggcggta atacggttat
     1801 ccacagaatc aggggataac gcaggaaaga acatgtgagc aaaaggccag caaaaggcca
     1861 ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag gctccgcccc cctgacgagc
     1921 atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc gacaggacta taaagatacc
     1981 aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg
     2041 gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc tcacgctgta
     2101 ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg
     2161 ttcagcccga ccgctgcgcc ttatccggta actatcgtct tgagtccaac ccggtaagac
     2221 acgacttatc gccactggca gcagccactg gtaacaggat tagcagagcg aggtatgtag
     2281 gcggtgctac agagttcttg aagtggtggc ctaactacgg ctacactaga aggacagtat
     2341 ttggtatctg cgctctgctg aagccagtta ccttcggaaa aagagttggt agctcttgat
     2401 ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag cagattacgc
     2461 gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc tacggggtct gacgctcagt
     2521 ggaacgaaaa ctcacgttaa gggattttgg tcatgagatt atcaaaaagg atcttcacct
     2581 agatcctttt aaattaaaaa tgaagtttta aatcaatcta aagtatatat gagtaaactt
     2641 ggtctgacag ttaccaatgc ttaatcagtg aggcacctat ctcagcgatc tgtctatttc
     2701 gttcatccat agttgcctga ctccccgtcg tgtagataac tacgatacgg gagggcttac
     2761 catctggccc cagtgctgca atgataccgc gagacccacg ctcaccggct ccagatttat
     2821 cagcaataaa ccagccagcc ggaagggccg agcgcagaag tggtcctgca actttatccg
     2881 cctccatcca gtctattaat tgttgccggg aagctagagt aagtagttcg ccagttaata
     2941 gtttgcgcaa cgttgttgcc attgctacag gcatcgtggt gtcacgctcg tcgtttggta
     3001 tggcttcatt cagctccggt tcccaacgat caaggcgagt tacatgatcc cccatgttgt
     3061 gcaaaaaagc ggttagctcc ttcggtcctc cgatcgttgt cagaagtaag ttggccgcag
     3121 tgttatcact catggttatg gcagcactgc ataattctct tactgtcatg ccatccgtaa
     3181 gatgcttttc tgtgactggt gagtactcaa ccaagtcatt ctgagaatag tgtatgcggc
     3241 gaccgagttg ctcttgcccg gcgtcaatac gggataatac cgcgccacat agcagaactt
     3301 taaaagtgct catcattgga aaacgttctt cggggcgaaa actctcaagg atcttaccgc
     3361 tgttgagatc cagttcgatg taacccactc gtgcacccaa ctgatcttca gcatctttta
     3421 ctttcaccag cgtttctggg tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa
     3481 taagggcgac acggaaatgt tgaatactca tactcttcct ttttcaatat tattgaagca
     3541 tttatcaggg ttattgtctc atgagcggat acatatttga atgtatttag aaaaataaac
     3601 aaataggggt tccgcgcaca tttccccgaa aagtgccac
//
