LOCUS       sequence-84022-e         890 bp    DNA     linear   UNA 15-APR-2025
DEFINITION  AmpR + PLlacO-1::RBS (st7) fimB::Asp terminator + ColE1.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 890)
  AUTHORS   Hung M, Chang E, Hussein R, Frazier K, Shin JE, Sagawa S, Lim HN
  TITLE     Modulating the frequency and bias of stochastic switching to control
            phenotypic variation.
  JOURNAL   Nat Commun. 2014 Aug 4;5:4574. doi: 10.1038/ncomms5574.
  PUBMED    25087841
REFERENCE   2  (bases 1 to 890)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2014-08-4"; volume: "5:4574. doi"; pages: " 
            10.1038/ncomms5574"
COMMENT     Sequence Label: pHL 1454
FEATURES             Location/Qualifiers
     source          1..890
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    650..666
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        724..828
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 ccctgtacga ttaaagcttc tataaaacag cgtgacgctg tcgtcctctg gctctatccc
       61 agatgccgta aaaacgccct gcattgctgg cggtatacca gacagtatga cgaatattgc
      121 gatgcccaag ataatcctgg ataagtcgcg tatctattcc catattcgcc aaagcaaaac
      181 cacacgaatg gcgtaacatg tgcggatgaa tctccagtga caacccggca ttaccaccgg
      241 aagtcgagat aatatggtaa aactgttgcc gagaaagcgg attcccctta cgtgataaaa
      301 atacccactc gctctcagca tgcgggtacg aagtacggat actcaaccag ttttttaaag
      361 cctgaacttc tttattcaat agcgggtgcg ttgttgaaaa gccttttttt aatcgatgga
      421 tatatataca ctttgcctta agatcaatat ccgaaatcct caatcgacaa atttcactcg
      481 cccggaaacc atgaataaaa caaagcaaag tcagacaata attacgtgct gcatgaggcc
      541 cggtatttgc tgctttaagg agtgattcga tttcactatg ggtcaggaag ttcctttttt
      601 tgttatcagc cttattcttc attttttttc ctccttaccc gggagtatct tgttatccgc
      661 tcacaatgtc aatgttatcc gctcacattt atgacgtcag gtggcacttt tcggggaaat
      721 gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg
      781 agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa
      841 catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt
//
