LOCUS       sequence-84081-e         871 bp    DNA     linear   UNA 14-APR-2025
DEFINITION  expression of nuclear LacZ driven by Gfa2 promoter with 3 additional
            copies of the gfa28 ABD segment; has about 100 times higher activity
            than pGfa2-nLac in transfected cells .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 871)
  AUTHORS   de Leeuw B, Su M, ter Horst M, Iwata S, Rodijk M, Hoeben RC, Messing
            A, Smitt PS, Brenner M
  TITLE     Increased glia-specific transgene expression with glial fibrillary 
            acidic protein promoters containing multiple enhancer elements.
  JOURNAL   J Neurosci Res. 2006 Apr;83(5):744-53.
  PUBMED    16496373
REFERENCE   2  (bases 1 to 871)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 14, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Neurosci 
            Res."; date: "2006-04"; volume: "83(5)"; pages: "744-53"
COMMENT     Sequence Label: pGfa2(ABDx3)-nLac
FEATURES             Location/Qualifiers
     source          1..871
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     161..183
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     CDS             complement(204..224)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
ORIGIN
        1 ggcaccgctt ctggtgccgg aaaccaggca aagcgccatt cgccattcag gctgcgcaac
       61 tgttgggaag ggcgatcggt gcgggcctct tcgctattac gccagctggc gaaaggggga
      121 tgtgctgcaa ggcgattaag ttgggtaacg ccagggtttt cccagtcacg acgttgtaaa
      181 acgacgggat cccccatgct ccccactttg cgtttcttct tgggccccat ggtggatcct
      241 gagactgggg aattccccgg atccgtcgac tctagaggat ccccgcgagc agcggaggtg
      301 atgcgtctcc tctccaacct gctctggctc tgctcgctcc tgggatgcga gggctttatg
      361 aaggagtggg ctagactggc gatgcccggg tgcccctggc aacaccccct ggcatagcct
      421 gagggggtgg ggttgggccg cagcccagct atggggagag ctccctgctc aatgggcttc
      481 tcggaaggca tttgaggggg gctggggtgc ggcgggaagc aggcactgtg cccaccccga
      541 acattgtgtc tgtgccaagg tgagtcattc actgggcatg aagaggaggc cctggggggc
      601 ggccagccca atgctgccag gtctgcttgc agacaaggcc tggctgtctg cctcctcccc
      661 agctcacaga gccagctcag gcccccgagc ctctgtcaga gcagcgtccc ctactccaca
      721 ccaggatatg ttagatcggg tgcccctggc aacaccccct ggcatagcct gagggggtgg
      781 ggttgggccg cagcccagct atggggagag ctccctgctc aatgggcttc tcggaaggca
      841 tttgaggggg gctggggtgc ggcgggaagc a
//
