LOCUS       sequence-84201-e        2622 bp    DNA     linear   UNA 15-APR-2025
DEFINITION  Localization: Membrane Receptor, Excitation: 487, Emission: 509.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2622)
  TITLE     Davidson mEmerald
REFERENCE   2  (bases 1 to 2622)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: mEmerald-ATG16-N-18
FEATURES             Location/Qualifiers
     source          1..2622
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      1856..1865
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(1862..1864,1865..1867,1868..2581)
                     /codon_start=1
                     /product="brightly fluorescent monomeric variant of GFP"
                     /label=mEmerald
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHKVYITADKQKNGIK
                     VNFKTRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(1907..1928)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(2168..2187)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     2515..2536
                     /label=EGFP-C
                     /note="EGFP, forward primer"
ORIGIN
        1 gctagcgcta ccggactcag atctcgagct caagcttacc gccatgtcgt cgggcctgcg
       61 cgccgcagac tttccccgct ggaagcgtca catcgcggag gaactgaggc gccgggaccg
      121 actgcagagg caggcgttcg aggagatcat tctgcagtat accaagttgc tggaaaagtc
      181 agatcttcat tcagtattga cccagaaact acaagcagaa aagcatgaca tgccaaatag
      241 gcatgaaata agtcctggac atgatggtgc gtggaatgat agtcaactac aagaaatggc
      301 ccagttgagg atcaaacacc aggaagagct gaccgaactg cacaagaagc gtggggagtt
      361 agctcagttg gtgattgacc tgaacaacca aatgcagcag aaggacaagg agatacagat
      421 gaatgaagca aagatttcgg agtatttaca gaccatctct gacctggaga caaactgcct
      481 ggacctgcgc accaaactgc aggacctcga ggtagccaac cagaccctga aggatgagta
      541 tgacgccctg cagattactt ttactgccct agaagagaaa ctgaggaaaa ctactgagga
      601 gaaccaggaa ctggtcacca gatggatggc tgagaaggcc caagaagcca atcgcctcaa
      661 tgcagagaat gagaaggact ccaggaggcg tcaagcacgg ctgcagaagg agcttgcaga
      721 agcagcaaag gaacctctac ctgttgaaca ggatgatgac attgaagtca ttgtggatga
      781 gacctcagac cacacagaag agacctctcc cgtccgagct gtcagcagag cagctacgag
      841 acgctctgtc tcttccatcc cagtccccca ggatatcatg gacactcatc ctgcttctgg
      901 taaagatgtg agagtcccaa ctactgcctc gtatgtcttc gatgcgcatg acggagaggt
      961 caacgcagtg cagttcagtc caggctcccg gttgctggcc actggaggca tggaccgcag
     1021 ggtgaaactt tgggaagcat tcggagataa atgtgaattc aagggctccc tgtctggcag
     1081 taatgctgga attacaagca ttgaatttga tagtgctgga gcttacctat tagcagcttc
     1141 aaatgatttt gcaagccgaa tctggactgt ggatgattat cgattacggc acacactcac
     1201 aggccacagc gggaaagtcc tctctgccaa gttcctgctg gacaatgcac ggattgtctc
     1261 aggaagtcac gaccggaccc tcaaactctg ggatctccgc agcaaagtct gcataaaaac
     1321 agtgtttgca ggatccagct gcaatgacat tgtttgcact gaacaatgtg taatgagtgg
     1381 acattttgac aagaaaattc gtttctggga tatccggtca gagagtgtgg tccgagagat
     1441 ggaactgtta gggaagatca ctgctctgga cctaaaccct gagagaactg agctcctgag
     1501 ctgctcccgt gatgacctgc taaaagtcat cgacctccgg acaaatgcag tcaaacagac
     1561 attcagtgcg cctggattca aatgcggctc tgactggacc cgggttgtct tcagccctga
     1621 tggcagttac gtggcagcag gctcagccga gggttctctt tatgtctgga gtgtgctgac
     1681 agggaaagtg gagaaggttc tttcaaaaca gcacagctct tctatcaatg cggtggcgtg
     1741 ggccccctcg ggcttacatg ttgtcagtgt ggacaaagga agcagagcta tgctgtgggc
     1801 acagcctggg agaggcagcg ggagtggcag tcgcggaggc agtggtggac cggtcgccac
     1861 catggtgagc aagggcgagg agctgttcac cggggtggtg cccatcctgg tcgagctgga
     1921 cggcgacgta aacggccaca agttcagcgt gtccggcgag ggcgagggcg atgccaccta
     1981 cggcaagctg accctgaagt tcatctgcac caccggcaag ctgcccgtgc cctggcccac
     2041 cctcgtgacc accttgacct acggcgtgca gtgcttcgcc cgctaccccg accacatgaa
     2101 gcagcacgac ttcttcaagt ccgccatgcc cgaaggctac gtccaggagc gcaccatctt
     2161 cttcaaggac gacggcaact acaagacccg cgccgaggtg aagttcgagg gcgacaccct
     2221 ggtgaaccgc atcgagctga agggcatcga cttcaaggag gacggcaaca tcctggggca
     2281 caagctggag tacaactaca acagccacaa ggtctatatc accgccgaca agcagaagaa
     2341 cggcatcaag gtgaacttca agacccgcca caacatcgag gacggcagcg tgcagctcgc
     2401 cgaccactac cagcagaaca cccccatcgg cgacggcccc gtgctgctgc ccgacaacca
     2461 ctacctgagc acccagtcca agctgagcaa agaccccaac gagaagcgcg atcacatggt
     2521 cctgctggag ttcgtgaccg ccgccgggat cactctcggc atggacgagc tgtacaagta
     2581 agcggccgcg actctagatc ataatcagcc ataccacatt gt
//
