LOCUS       sequence-84271-e        8795 bp    DNA     linear   UNA 15-APR-2025
DEFINITION  Localization: Actin/Focal Adhesions, Excitation: 487, Emission: 509.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 8795)
  TITLE     Davidson mEmerald
REFERENCE   2  (bases 1 to 8795)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: mEmerald-FilaminA-C-14
FEATURES             Location/Qualifiers
     source          1..8795
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      28..37
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(34..36,37..39,40..750)
                     /codon_start=1
                     /product="brightly fluorescent monomeric variant of GFP"
                     /label=mEmerald
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHKVYITADKQKNGIK
                     VNFKTRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(79..100)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(340..359)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     687..708
                     /label=EGFP-C
                     /note="EGFP, forward primer"
ORIGIN
        1 acgtcagatc cgctagcgct accggtcgcc accatggtga gcaagggcga ggagctgttc
       61 accggggtgg tgcccatcct ggtcgagctg gacggcgacg taaacggcca caagttcagc
      121 gtgtccggcg agggcgaggg cgatgccacc tacggcaagc tgaccctgaa gttcatctgc
      181 accaccggca agctgcccgt gccctggccc accctcgtga ccaccttgac ctacggcgtg
      241 cagtgcttcg cccgctaccc cgaccacatg aagcagcacg acttcttcaa gtccgccatg
      301 cccgaaggct acgtccagga gcgcaccatc ttcttcaagg acgacggcaa ctacaagacc
      361 cgcgccgagg tgaagttcga gggcgacacc ctggtgaacc gcatcgagct gaagggcatc
      421 gacttcaagg aggacggcaa catcctgggg cacaagctgg agtacaacta caacagccac
      481 aaggtctata tcaccgccga caagcagaag aacggcatca aggtgaactt caagacccgc
      541 cacaacatcg aggacggcag cgtgcagctc gccgaccact accagcagaa cacccccatc
      601 ggcgacggcc ccgtgctgct gcccgacaac cactacctga gcacccagtc caagctgagc
      661 aaagacccca acgagaagcg cgatcacatg gtcctgctgg agttcgtgac cgccgccggg
      721 atcactctcg gcatggacga gctgtacaag tccggaggga gcggggctgg agggagcggg
      781 ggtggcagcg gtagtagctc ccactctcgg gcgggccaga gcgcagcagg cgcggctccg
      841 ggcggcggcg tcgacacgcg ggacgccgag atgccggcca ccgagaagga cctggcggag
      901 gacgcgccat ggaagaagat ccagcagaac actttcacgc gctggtgcaa cgagcacctg
      961 aagtgcgtga gcaagcgcat cgccaacctg cagacggacc tgagcgacgg gctgcggctt
     1021 atcgcgctgt tggaggtgct cagccagaag aagatgcacc gcaagcacaa ccagcggccc
     1081 actttccgcc aaatgcagct tgagaacgtg tcggtggcac tcgagttcct ggaccgcgag
     1141 agcatcaaac tggtgtccat cgacagcaag gccatcgtgg acgggaacct gaagctgatc
     1201 ctgggcctca tctggaccct gatcctgcac tactccatct ccatgcccat gtgggacgag
     1261 gaggaggatg aggaggccaa gaagcagacc cccaagcaga ggctcctggg ctggatccag
     1321 aacaagctgc cgcagctgcc catcaccaac ttcagccggg actggcagag cggccgggcc
     1381 ctgggcgccc tggtggacag ctgtgccccg ggcctgtgtc ctgactggga ctcttgggac
     1441 gccagcaagc ccgttaccaa tgcgcgagag gccatgcagc aggcggatga ctggctgggc
     1501 atcccccagg tgatcacccc cgaggagatt gtggacccca acgtggacga gcactctgtc
     1561 atgacctacc tgtcccagtt ccccaaggcc aagctgaagc caggggctcc cttgcggccc
     1621 aaactgaacc cgaagaaagc ccgtgcctac gggccaggca tcgagcccac aggcaacatg
     1681 gtgaagaagc gggcagagtt cactgtggag accagaagtg ctggccaggg agaggtgctg
     1741 gtgtacgtgg aggacccggc cggacaccag gaggaggcaa aagtgaccgc caataacgac
     1801 aagaaccgca ccttctccgt ctggtacgtc cccgaggtga cggggactca taaggttact
     1861 gtgctctttg ctggccagca catcgccaag agccccttcg aggtgtacgt ggataagtca
     1921 cagggtgacg ccagcaaagt gacagcccaa ggtcccggcc tggagcccag tggcaacatc
     1981 gccaacaaga ccacctactt tgagatcttt acggcaggag ctggcacggg cgaggtcgag
     2041 gttgtgatcc aggaccccat gggacagaag ggcacggtag agcctcagct ggaggcccgg
     2101 ggcgacagca cataccgctg cagctaccag cccaccatgg agggcgtcca caccgtgcac
     2161 gtcacgtttg ccggcgtgcc catccctcgc agcccctaca ctgtcactgt tggccaagcc
     2221 tgtaacccga gtgcctgccg ggcggttggc cggggcctcc agcccaaggg tgtgcgggtg
     2281 aaggagacag ctgacttcaa ggtgtacaca aagggcgctg gcagtgggga gctgaaggtc
     2341 accgtgaagg gccccaaggg agaggagcgc gtgaagcaga aggacctggg ggatggcgtg
     2401 tatggcttcg agtattaccc catggtccct ggaacctata tcgtcaccat cacgtggggt
     2461 ggtcagaaca tcgggcgcag tcccttcgaa gtgaaggtgg gcaccgagtg tggcaatcag
     2521 aaggtacggg cctggggccc tgggctggag ggcggcgtcg ttggcaagtc agcagacttt
     2581 gtggtggagg ctatcgggga cgacgtgggc acgctgggct tctcggtgga agggccatcg
     2641 caggctaaga tcgaatgtga cgacaagggc gacggctcct gtgatgtgcg ctactggccg
     2701 caggaggctg gcgagtatgc cgttcacgtg ctgtgcaaca gcgaagacat ccgcctcagc
     2761 cccttcatgg ctgacatccg tgacgcgccc caggacttcc acccagacag ggtgaaggca
     2821 cgtgggcctg gattggagaa gacaggtgtg gccgtcaaca agccagcaga gttcacagtg
     2881 gatgccaagc acggtggcaa ggccccactt cgggtccaag tccaggacaa tgaaggctgc
     2941 cctgtggagg cgttggtcaa ggacaacggc aatggcactt acagctgctc ctacgtgccc
     3001 aggaagccgg tgaagcacac agccatggtg tcctggggag gcgtcagcat ccccaacagc
     3061 cccttcaggg tgaatgtggg agctggcagc caccccaaca aggtcaaagt atacggcccc
     3121 ggagtagcca agacagggct caaggcccac gagcccacct acttcactgt ggactgcgcc
     3181 gaggctggcc agggggacgt cagcatcggc atcaagtgtg cccctggagt ggtaggcccc
     3241 gccgaagctg acatcgactt cgacatcatc cgcaatgaca atgacacctt cacggtcaag
     3301 tacacgcccc ggggggctgg cagctacacc attatggtcc tctttgctga ccaggccacg
     3361 cccaccagcc ccatccgagt caaggtggag ccctctcatg acgccagtaa ggtgaaggcc
     3421 gagggccctg gcctcagtcg cactggtgtc gagcttggca agcccaccca cttcacagta
     3481 aatgccaaag ctgctggcaa aggcaagctg gacgtccagt tctcaggact caccaagggg
     3541 gatgcagtgc gagatgtgga catcatcgac caccatgaca acacctacac agtcaagtac
     3601 acgcctgtcc agcagggtcc agtaggcgtc aatgtcactt atggagggga tcccatccct
     3661 aagagccctt tctcagtggc agtatctcca agcctggacc tcagcaagat caaggtgtct
     3721 ggcctgggag agaaggtgga cgttggcaaa gaccaggagt tcacagtcaa atcaaagggt
     3781 gctggtggtc aaggcaaagt ggcatccaag attgtgggcc cctcgggtgc agcggtgccc
     3841 tgcaaggtgg agccaggcct gggggctgac aacagtgtgg tgcgcttcct gccccgtgag
     3901 gaagggccct atgaggtgga ggtgacctat gacggcgtgc ccgtgcctgg cagccccttt
     3961 cctctggaag ctgtggcccc caccaagcct agcaaggtga aggcgtttgg gccggggctg
     4021 cagggaggca gtgcgggctc ccccgcccgc ttcaccatcg acaccaaggg cgccggcaca
     4081 ggtggcctgg gcctgacggt ggagggcccc tgtgaggcgc agctcgagtg cttggacaat
     4141 ggggatggca catgttccgt gtcctacgtg cccaccgagc ccggggacta caacatcaac
     4201 atcctcttcg ctgacaccca catccctggc tccccattca aggcccacgt ggttccctgc
     4261 tttgacgcat ccaaagtcaa gtgctcaggc cccgggctgg agcgggccac cgctggggag
     4321 gtgggccaat tccaagtgga ctgctcgagc gcgggcagcg cggagctgac cattgagatc
     4381 tgctcggagg cggggcttcc ggccgaggtg tacatccagg accacggtga tggcacgcac
     4441 accattacct acattcccct ctgccccggg gcctacaccg tcaccatcaa gtacggcggc
     4501 cagcccgtgc ccaacttccc cagcaagctg caggtggaac ctgcggtgga cacttccggt
     4561 gtccagtgct atgggcctgg tattgagggc cagggtgtct tccgtgaggc caccactgag
     4621 ttcagtgtgg acgcccgggc tctgacacag accggagggc cgcacgtcaa ggcccgtgtg
     4681 gccaacccct caggcaacct gacggagacc tacgttcagg accgtggcga tggcatgtac
     4741 aaagtggagt acacgcctta cgaggaggga ctgcactccg tggacgtgac ctatgacggc
     4801 agtcccgtgc ccagcagccc cttccaggtg cccgtgaccg agggctgcga cccctcccgg
     4861 gtgcgtgtcc acgggccagg catccaaagt ggcaccacca acaagcccaa caagttcact
     4921 gtggagacca ggggagctgg cacgggcggc ctgggcctgg ctgtagaggg cccctccgag
     4981 gccaagatgt cctgcatgga taacaaggac ggcagctgct cggtcgagta catcccttat
     5041 gaggctggca cctacagcct caacgtcacc tatggtggcc atcaagtgcc aggcagtcct
     5101 ttcaaggtcc ctgtgcatga tgtgacagat gcgtccaagg tcaagtgctc tgggcccggc
     5161 ctgagcccag gcatggttcg tgccaacctc cctcagtcct tccaggtgga cacaagcaag
     5221 gctggtgtgg ccccattgca ggtcaaagtg caagggccca aaggcctggt ggagccagtg
     5281 gacgtggtag acaacgctga tggcacccag accgtcaatt atgtgcccag ccgagaaggg
     5341 ccctacagca tctcagtact gtatggagat gaagaggtac cccggagccc cttcaaggtc
     5401 aaggtgctgc ctactcatga tgccagcaag gtgaaggcca gtggccccgg gctcaacacc
     5461 actggcgtgc ctgccagcct gcccgtggag ttcaccatcg atgcaaagga cgccggggag
     5521 ggcctgctgg ctgtccagat cacggatccc gaaggcaagc cgaagaagac acacatccaa
     5581 gacaaccatg acggcacgta tacagtggcc tacgtgccag acgtgacagg tcgctacacc
     5641 atcctcatca agtacggtgg tgacgagatc cccttctccc cgtaccgcgt gcgtgccgtg
     5701 cccaccgggg acgccagcaa gtgcactgtc acaggtgctg gcatcggccc caccattcag
     5761 attggggagg agacggtgat cactgtggac actaaggcgg caggcaaagg caaagtgacg
     5821 tgcaccgtgt gcacgcctga tggctcagag gtggatgtgg acgtggtgga gaatgaggac
     5881 ggcactttcg acatcttcta cacggccccc cagccgggca aatacgtcat ctgtgtgcgc
     5941 tttggtggcg agcacgtgcc caacagcccc ttccaagtga cggctctggc tggggaccag
     6001 ccctcggtgc agccccctct acggtctcag cagctggccc cacagtacac ctacgcccag
     6061 ggcggccagc agacttgggc cccggagagg cccctggtgg gtgtcaatgg gctggatgtg
     6121 accagcctga ggccctttga ccttgtcatc cccttcacca tcaagaaggg cgagatcaca
     6181 ggggaggttc ggatgccctc aggcaaggtg gcgcagccca ccatcactga caacaaagac
     6241 ggcaccgtga ccgtgcggta tgcacccagc gaggctggcc tgcacgagat ggacatccgc
     6301 tatgacaaca tgcacatccc aggaagcccc ttgcagttct atgtggatta cgtcaactgt
     6361 ggccatgtca ctgcctatgg gcctggcctc acccatggag tagtgaacaa gcctgccacc
     6421 ttcaccgtca acaccaagga tgcaggagag gggggcctgt ctctggccat tgagggcccg
     6481 tccaaagcag aaatcagctg cactgacaac caggatggga catgcagcgt gtcctacctg
     6541 cctgtgctgc cgggggacta cagcattcta gtcaagtaca atgaacagca cgtcccaggc
     6601 agccccttca ctgcccgggt cacaggtgac gactccatgc gtatgtccca cctaaaggtc
     6661 ggctctgctg ccgacatccc catcaacatc tcagagacgg atctcagcct gctgacggcc
     6721 actgtggtcc cgccctcggg ccgggaggag ccctgtttgc tgaagcggct gcgtaatggc
     6781 cacgtgggga tttcattcgt gcccaaggag acgggggagc acctggtgca tgtgaagaaa
     6841 aatggccagc acgtggccag cagccccatc ccggtggtga tcagccagtc ggaaattggg
     6901 gatgccagtc gtgttcgggt ctctggtcag ggccttcacg aaggccacac ctttgagcct
     6961 gcagagttta tcattgatac ccgcgatgca ggctatggtg ggctcagcct gtccattgag
     7021 ggccccagca aggtggacat caacacagag gacctggagg acgggacgtg cagggtcacc
     7081 tactgcccca cagagccagg caactacatc atcaacatca agtttgccga ccagcacgtg
     7141 cctggcagcc ccttctctgt gaaggtgaca ggcgagggcc gggtgaaaga gagcatcacc
     7201 cgcaggcgtc gggctccttc agtggccaac gttggtagtc attgtgacct cagcctgaaa
     7261 atccctgaaa ttagcatcca ggatatgaca gcccaggtga ccagcccatc gggcaagacc
     7321 catgaggccg agatcgtgga aggggagaac cacacctact gcatccgctt tgttcccgct
     7381 gagatgggca cacacacagt cagcgtcaag tacaagggcc agcacgtgcc tgggagcccc
     7441 ttccagttca ccgtggggcc cctaggggaa gggggagccc acaaggtccg agctgggggc
     7501 cctggcctgg agagagctga agctggagtg ccagccgaat tcagtatctg gacccgggaa
     7561 gctggtgctg gaggcctggc cattgctgtc gagggcccca gcaaggctga gatctctttt
     7621 gaggaccgca aggacggctc ctgtggtgtg gcttatgtgg tccaggagcc aggtgactac
     7681 gaagtctcag tcaagttcaa cgaggaacac attcccgaca gccccttcgt ggtgcctgtg
     7741 gcttctccgt ctggcgacgc ccgccgcctc actgtttcta gccttcagga gtcagggcta
     7801 aaggtcaacc agccagcctc ttttgcagtc agcctgaacg gggccaaggg ggcgatcgat
     7861 gccaaggtgc acagcccctc aggagccctg gaggagtgct atgtcacaga aattgaccaa
     7921 gataagtatg ctgtgcgctt catccctcgg gagaatggcg tttacctgat tgacgtcaag
     7981 ttcaacggta cccacatccc tggaagcccc ttcaagatcc gagttgggga gcctgggcat
     8041 ggaggggacc caggcttggt gtctgcttac ggagcaggtc tggaaggcgg tgtcacaggg
     8101 aacccagctg agttcgtcgt gaacacgagc aatgcgggag ctggtgccct gtcggtgacc
     8161 attgacggcc cctccaaggt gaagatggat tgccaggagt gccctgaggg ctaccgcgtc
     8221 acctataccc ccatggcacc tggcagctac ctcatctcca tcaagtacgg cggcccctac
     8281 cacattgggg gcagcccctt caaggccaaa gtcacaggcc cccgtctcgt cagcaaccac
     8341 agcctccacg agacatcatc agtgtttgta gactctctga ccaaggccac ctgtgccccc
     8401 cagcatgggg ccccgggtcc tgggcctgct gacgccagca aggtggtggc caagggcctg
     8461 gggctgagca aggcctacgt aggccagaag agcagcttca cagtagactg cagcaaagca
     8521 ggcaacaaca tgctgctggt gggggttcat ggcccaagga ccccctgcga ggagatcctg
     8581 gtgaagcacg tgggcagccg gctctacagc gtgtcctacc tgctcaagga caagggggag
     8641 tacacactgg tggtcaaatg ggggcacgag cacatcccag gcagccccta ccgcgttgtg
     8701 gtgccctaaa agcttcgaat tctgcagtcg acggtaccgc gggcccggga tccaccggat
     8761 ctagataact gatcataatc agccatacca cattt
//
