LOCUS       sequence-84350-e        1390 bp    DNA     linear   UNA 15-APR-2025
DEFINITION  Localization: Endosomes, Excitation: 487, Emission: 509.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1390)
  TITLE     Davidson mEmerald
REFERENCE   2  (bases 1 to 1390)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: mEmerald-NEEP21-N-18
FEATURES             Location/Qualifiers
     source          1..1390
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      17..26
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             complement(107..118)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     CDS             complement(128..139)
                     /codon_start=1
                     /product="WELQut protease recognition and cleavage site"
                     /label=WELQut site
                     /translation="WELQ"
     regulatory      626..635
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(632..634,635..637,638..1351)
                     /codon_start=1
                     /product="brightly fluorescent monomeric variant of GFP"
                     /label=mEmerald
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHKVYITADKQKNGIK
                     VNFKTRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(677..698)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(938..957)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     1285..1306
                     /label=EGFP-C
                     /note="EGFP, forward primer"
ORIGIN
        1 cgtcagatcc gctagcgcca ccatggtgaa gttggggaat aatttcgcag agaagggcac
       61 caagcagcca ctgctggagg atggcttcga caccattcct ttgatgacgc ccctcgatgt
      121 caaccagctg cagttcccac ccccagataa ggtcgtggtg aaaactaaga ctgaatatga
      181 acctgatcgc aaaaaaggaa aagcacgtcc tcccaagata gccgagttca ccgtcagcat
      241 caccgagggt gtcaccgaga ggtttaaggt ctccgtgctg gtcctctttg ccctggcctt
      301 cctcacctgt gtcgtcttcc tggttgtcta caaagtgtac aagtatgacc gcgcctgccc
      361 tggtgggttt gtcttgaaga acacccagtg catcccagaa ggcttggaga gctactacac
      421 ggagcaagac tccagtgccc gggagaaatt ttacactgtc ataaaccact acaacgtggc
      481 caagcagagc atcacccgct ccgtgtcgcc atggatgtca gttctgtcag aagagaagct
      541 gtcggaacag gagaccgaag ctgcagagaa gtcagccagc agcggtggag caagcgcagc
      601 cagtggtagc gcggatccac cggtcgccac catggtgagc aagggcgagg agctgttcac
      661 cggggtggtg cccatcctgg tcgagctgga cggcgacgta aacggccaca agttcagcgt
      721 gtccggcgag ggcgagggcg atgccaccta cggcaagctg accctgaagt tcatctgcac
      781 caccggcaag ctgcccgtgc cctggcccac cctcgtgacc accttgacct acggcgtgca
      841 gtgcttcgcc cgctaccccg accacatgaa gcagcacgac ttcttcaagt ccgccatgcc
      901 cgaaggctac gtccaggagc gcaccatctt cttcaaggac gacggcaact acaagacccg
      961 cgccgaggtg aagttcgagg gcgacaccct ggtgaaccgc atcgagctga agggcatcga
     1021 cttcaaggag gacggcaaca tcctggggca caagctggag tacaactaca acagccacaa
     1081 ggtctatatc accgccgaca agcagaagaa cggcatcaag gtgaacttca agacccgcca
     1141 caacatcgag gacggcagcg tgcagctcgc cgaccactac cagcagaaca cccccatcgg
     1201 cgacggcccc gtgctgctgc ccgacaacca ctacctgagc acccagtcca agctgagcaa
     1261 agaccccaac gagaagcgcg atcacatggt cctgctggag ttcgtgaccg ccgccgggat
     1321 cactctcggc atggacgagc tgtacaagta agcggccgcg actctagatc ataatcagcc
     1381 ataccacatt
//
