LOCUS       sequence-84357-e        2378 bp    DNA     linear   UNA 14-APR-2025
DEFINITION  Localization: Tight Junctions, Excitation: 487, Emission: 509.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2378)
  TITLE     Davidson mEmerald
REFERENCE   2  (bases 1 to 2378)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 14, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: mEmerald-Occludin-N-10
FEATURES             Location/Qualifiers
     source          1..2378
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             50..58
                     /label=Shine-Dalgarno sequence
                     /note="full consensus sequence for ribosome-binding sites 
                     upstream of start codons in E. coli; complementary to a 
                     region in the 3' end of the 16S rRNA (Chen et al., 1994)"
     regulatory      1614..1623
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(1620..1622,1623..1625,1626..2339)
                     /codon_start=1
                     /product="brightly fluorescent monomeric variant of GFP"
                     /label=mEmerald
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHKVYITADKQKNGIK
                     VNFKTRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(1665..1686)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(1926..1945)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     2273..2294
                     /label=EGFP-C
                     /note="EGFP, forward primer"
ORIGIN
        1 acgtcagatc cgctagcgcc accatgtcat ccaggcctct tgaaagtcca cctccttaca
       61 ggcctgatga attcaaaccg aatcattatg caccaagcaa tgacatatat ggtggagaga
      121 tgcatgttcg accaatgctc tctcagccag cctactcttt ttacccagaa gatgaaattc
      181 ttcacttcta caaatggacc tctcctccag gagtgattcg gatcctgtct atgctcatta
      241 ttgtgatgtg cattgccatc tttgcctgtg tggcctccac gcttgcctgg gacagaggct
      301 atggaacttc ccttttagga ggtagtgtag gctaccctta tggaggaagt ggctttggta
      361 gctacggaag tggctatggc tatggctatg gttatggcta tggctacgga ggctatacag
      421 acccaagagc agcaaagggc ttcatgttgg ccatggctgc cttttgtttc attgccgcgt
      481 tggtgatctt tgttaccagt gttataagat ctgaaatgtc cagaacaaga agatactact
      541 taagtgtgat aatagtgagt gctatcctgg gcatcatggt gtttattgcc acaattgtct
      601 atataatggg agtgaaccca actgctcagt cttctggatc tctatatggt tcacaaatat
      661 atgccctctg caaccaattt tatacacctg cagctactgg actctacgtg gatcagtatt
      721 tgtatcacta ctgtgttgtg gatccccagg aggccattgc cattgtactg gggttcatga
      781 ttattgtggc ttttgcttta ataattttct ttgctgtgaa aactcgaaga aagatggaca
      841 ggtatgacaa gtccaatatt ttgtgggaca aggaacacat ttatgatgag cagcccccca
      901 atgtcgagga gtgggttaaa aatgtgtctg caggcacaca ggacgtgcct tcacccccat
      961 ctgactatgt ggaaagagtt gacagtccca tggcatactc ttccaatggc aaagtgaatg
     1021 acaagcggtt ttatccagag tcttcctata aatccacgcc ggttcctgaa gtggttcagg
     1081 agcttccatt aacttcgcct gtggatgact tcaggcagcc tcgttacagc agcggtggta
     1141 actttgagac accttcaaaa agagcacctg caaagggaag agcaggaagg tcaaagagaa
     1201 cagagcaaga tcactatgag acagactaca caactggcgg cgagtcctgt gatgagctgg
     1261 aggaggactg gatcagggaa tatccaccta tcacttcaga tcaacaaaga caactgtaca
     1321 agaggaattt tgacactggc ctacaggaat acaagagctt acaatcagaa cttgatgaga
     1381 tcaataaaga actctcccgt ttggataaag aattggatga ctatagagaa gaaagtgaag
     1441 agtacatggc tgctgctgat gaatacaata gactgaagca agtgaaggga tctgcagatt
     1501 acaaaagtaa gaagaatcat tgcaagcagt taaagagcaa attgtcacac atcaagaaga
     1561 tggttggaga ctatgataga cagaaaacaa gcggctccgg tagcggaccg gtcgccacca
     1621 tggtgagcaa gggcgaggag ctgttcaccg gggtggtgcc catcctggtc gagctggacg
     1681 gcgacgtaaa cggccacaag ttcagcgtgt ccggcgaggg cgagggcgat gccacctacg
     1741 gcaagctgac cctgaagttc atctgcacca ccggcaagct gcccgtgccc tggcccaccc
     1801 tcgtgaccac cttgacctac ggcgtgcagt gcttcgcccg ctaccccgac cacatgaagc
     1861 agcacgactt cttcaagtcc gccatgcccg aaggctacgt ccaggagcgc accatcttct
     1921 tcaaggacga cggcaactac aagacccgcg ccgaggtgaa gttcgagggc gacaccctgg
     1981 tgaaccgcat cgagctgaag ggcatcgact tcaaggagga cggcaacatc ctggggcaca
     2041 agctggagta caactacaac agccacaagg tctatatcac cgccgacaag cagaagaacg
     2101 gcatcaaggt gaacttcaag acccgccaca acatcgagga cggcagcgtg cagctcgccg
     2161 accactacca gcagaacacc cccatcggcg acggccccgt gctgctgccc gacaaccact
     2221 acctgagcac ccagtccaag ctgagcaaag accccaacga gaagcgcgat cacatggtcc
     2281 tgctggagtt cgtgaccgcc gccgggatca ctctcggcat ggacgagctg tacaagtaag
     2341 cggccgcgac tctagatcat aatcagccat accacatt
//
