LOCUS       sequence-84363-e        2505 bp    DNA     linear   UNA 15-APR-2025
DEFINITION  Localization: Focal Adhesions, Excitation: 487, Emission: 509.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2505)
  TITLE     Davidson mEmerald
REFERENCE   2  (bases 1 to 2505)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: mEmerald-Paxillin-KM-N-18
FEATURES             Location/Qualifiers
     source          1..2505
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      1751..1760
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(1757..1759,1760..1762,1763..2476)
                     /codon_start=1
                     /product="brightly fluorescent monomeric variant of GFP"
                     /label=mEmerald
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHKVYITADKQKNGIK
                     VNFKTRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(1802..1823)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(2063..2082)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     2410..2431
                     /label=EGFP-C
                     /note="EGFP, forward primer"
ORIGIN
        1 cgtcagatcc gctagcgcca ccatgatgga cgacctcgat gccttactgg cagacctgga
       61 atccaccacc tcgcatatct ctaagcgacc ggtgtttcta acagaggaaa cgccttactc
      121 ctacccaact ggaaaccata cgtaccagga gattgctgtg ccaccgccgg tgcccccgcc
      181 gccttccagt gaggccctaa atggcactgt gattgacccc ttagaccagt ggcaacccag
      241 cgtgtccaga tacggccacc agcaacctca gtcccagtct ccgatataca gctctagtgc
      301 caaaagctcc agtgcctctg ttcctagaga cgggctcagc tccccttctc cccgtgccag
      361 tgaggaggaa cacgtgtaca gcttcccaaa caagcagaag tctgcagaac catctccctc
      421 actgaccagc acctccctgg gcagcaacct ctcggaactg gacaggcttc tcctggaact
      481 gaatgctgtt caacataatc cccccagtgg cttctcagca gatgaagtca gcagaagccc
      541 atcactgccc aatgtgaccg gacctcacta tgtcatccca gagagcagca gctctgcagg
      601 agggaaggct gcacccccta caaaagaaaa accaaagcgg aatggtgggc gtgggattga
      661 agatgtgcgt cccagtgtgg agagcctgct ggatgagctg gagagctctg tgccaagccc
      721 agtccctgca atcactgtga gccaagggga ggtgagcagc ccccagcgtg tcaacgccag
      781 tcagcagcag acccgtatct ctgcttcttc agctacacga gaactggatg agctgatggc
      841 gtccctctct gactttaagt tcatggcaca ggggaaagcc ggcggcagca gctccccacc
      901 atccaccacc ccaaagcctg gcagtcagct ggacaccatg ctgggaagtc tgcagtctga
      961 cctgaacaaa ctgggggtag cgacggtcgc caagggtgtg tgcggagcct gcaagaagcc
     1021 gattgctggg caggtagtta cagccatggg gaagacctgg caccctgagc acttcgtctg
     1081 cacccactgc caggaggaga ttggatcacg gaacttcttt gagcgggatg ggcagcccta
     1141 ctgtgagaag gactatcaca acctcttctc tcctcgctgc tactactgca atgggccaat
     1201 ccttgataaa gtggtgacgg ctttggacag gacgtggcac cctgaacact ttttctgtgc
     1261 ccagtgtgga gttttctttg gacctgaagg atttcatgag aaggatggca aagcctattg
     1321 ccgcaaggac tactttgaca tgtttgctcc caagtgtgga ggctgtgccc gggctatcct
     1381 ggaaaattac atttctgcct taaacaccct gtggcaccct gagtgctttg tctgtcggga
     1441 atgtttcaca ccgttcatca acggcagctt cttcgagcac gacggccagc cctactgcga
     1501 ggtgcattac cacgagcgcc gcggctcgct gtgctctggc tgccagaagc ccatcacggg
     1561 acgctgcatc actgccatgg gcaagaaatt tcaccccgaa cactttgtct gtgccttctg
     1621 cctcaagcag ctcaacaaag gaaccttcaa agaacagaac gacaagccct actgtcagaa
     1681 ctgctttctc aaactcttct gtagcagcgg tggagcaagc gcagccagtg gtagcgcgga
     1741 tccaccggtc gccaccatgg tgagcaaggg cgaggagctg ttcaccgggg tggtgcccat
     1801 cctggtcgag ctggacggcg acgtaaacgg ccacaagttc agcgtgtccg gcgagggcga
     1861 gggcgatgcc acctacggca agctgaccct gaagttcatc tgcaccaccg gcaagctgcc
     1921 cgtgccctgg cccaccctcg tgaccacctt gacctacggc gtgcagtgct tcgcccgcta
     1981 ccccgaccac atgaagcagc acgacttctt caagtccgcc atgcccgaag gctacgtcca
     2041 ggagcgcacc atcttcttca aggacgacgg caactacaag acccgcgccg aggtgaagtt
     2101 cgagggcgac accctggtga accgcatcga gctgaagggc atcgacttca aggaggacgg
     2161 caacatcctg gggcacaagc tggagtacaa ctacaacagc cacaaggtct atatcaccgc
     2221 cgacaagcag aagaacggca tcaaggtgaa cttcaagacc cgccacaaca tcgaggacgg
     2281 cagcgtgcag ctcgccgacc actaccagca gaacaccccc atcggcgacg gccccgtgct
     2341 gctgcccgac aaccactacc tgagcaccca gtccaagctg agcaaagacc ccaacgagaa
     2401 gcgcgatcac atggtcctgc tggagttcgt gaccgccgcc gggatcactc tcggcatgga
     2461 cgagctgtac aagtaagcgg ccgcgactct agatcataat cagcc
//
