LOCUS       sequence-84379-e        2641 bp    DNA     linear   UNA 14-APR-2025
DEFINITION  Localization: Centrosomes, Excitation: 487, Emission: 509.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2641)
  TITLE     Davidson mEmerald
REFERENCE   2  (bases 1 to 2641)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 14, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: mEmerald-PLK1-N-16
FEATURES             Location/Qualifiers
     source          1..2641
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      1877..1886
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(1883..1885,1886..1888,1889..2602)
                     /codon_start=1
                     /product="brightly fluorescent monomeric variant of GFP"
                     /label=mEmerald
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHKVYITADKQKNGIK
                     VNFKTRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(1928..1949)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(2189..2208)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     2536..2557
                     /label=EGFP-C
                     /note="EGFP, forward primer"
ORIGIN
        1 gacggtcaga tccgctagcg ccaccatgag tgctgcagtg actgcaggga agctggcacg
       61 ggcaccggcc gaccctggga aagccggggt ccccggagtt gcagctcccg gagctccggc
      121 ggcggctcca ccggcgaaag agatcccgga ggtcctagtg gacccacgca gccggcggcg
      181 ctatgtgcgg ggccgctttt tgggcaaggg cggctttgcc aagtgcttcg agatctcgga
      241 cgcggacacc aaggaggtgt tcgcgggcaa gattgtgcct aagtctctgc tgctcaagcc
      301 gcaccagagg gagaagatgt ccatggaaat atccattcac cgcagcctcg cccaccagca
      361 cgtcgtagga ttccacggct ttttcgagga caacgacttc gtgttcgtgg tgttggagct
      421 ctgccgccgg aggtctctcc tggagctgca caagaggagg aaagccctga ctgagcctga
      481 ggcccgatac tacctacggc aaattgtgct tggctgccag tacctgcacc gaaaccgagt
      541 tattcatcga gacctcaagc tgggcaacct tttcctgaat gaagatctgg aggtgaaaat
      601 aggggatttt ggactggcaa ccaaagtcga atatgacggg gagaggaaga agaccctgtg
      661 tgggactcct aattacatag ctcccgaggt gctgagcaag aaagggcaca gtttcgaggt
      721 ggatgtgtgg tccattgggt gtatcatgta taccttgtta gtgggcaaac caccttttga
      781 gacttcttgc ctaaaagaga cctacctccg gatcaagaag aatgaataca gtattcccaa
      841 gcacatcaac cccgtggccg cctccctcat ccagaagatg cttcagacag atcccactgc
      901 ccgcccaacc attaacgagc tgcttaatga cgagttcttt acttctggct atatccctgc
      961 ccgtctcccc atcacctgcc tgaccattcc accaaggttt tcgattgctc ccagcagcct
     1021 ggaccccagc aaccggaagc ccctcacagt cctcaataaa ggcttggaga accccctgcc
     1081 tgagcgtccc cgggaaaaag aagaaccagt ggttcgagag acaggtgagg tggtcgactg
     1141 ccacctcagt gacatgctgc agcagctgca cagtgtcaat gcctccaagc cctcggagcg
     1201 tgggctggtc aggcaagagg aggctgagga tcctgcctgc atccccatct tctgggtcag
     1261 caagtgggtg gactattcgg acaagtacgg ccttgggtat cagctctgtg ataacagcgt
     1321 gggggtgctc ttcaatgact caacacgcct catcctctac aatgatggtg acagcctgca
     1381 gtacatagag cgtgacggca ctgagtccta cctcaccgtg agttcccatc ccaactcctt
     1441 gatgaagaag atcaccctcc ttaaatattt ccgcaattac atgagcgagc acttgctgaa
     1501 ggcaggtgcc aacatcacgc cgcgcgaagg tgatgagctc gcccggctgc cctacctacg
     1561 gacctggttc cgcacccgca gcgccatcat cctgcacctc agcaacggca gcgtgcagat
     1621 caacttcttc caggatcaca ccaagctcat cttgtgccca ctgatggcag ccgtgaccta
     1681 catcgacgag aagcgggact tccgcacata ccgcctgagt ctcctggagg agtacggctg
     1741 ctgcaaggag ctggccagcc ggctccgcta cgcccgcact atggtggaca agctgctgag
     1801 ctcacgctcg gccagcaacc gtctcaaggc ctccggtagc ggcagcgcat ccggcggaag
     1861 cggaagcgga ccggtcgcca ccatggtgag caagggcgag gagctgttca ccggggtggt
     1921 gcccatcctg gtcgagctgg acggcgacgt aaacggccac aagttcagcg tgtccggcga
     1981 gggcgagggc gatgccacct acggcaagct gaccctgaag ttcatctgca ccaccggcaa
     2041 gctgcccgtg ccctggccca ccctcgtgac caccttgacc tacggcgtgc agtgcttcgc
     2101 ccgctacccc gaccacatga agcagcacga cttcttcaag tccgccatgc ccgaaggcta
     2161 cgtccaggag cgcaccatct tcttcaagga cgacggcaac tacaagaccc gcgccgaggt
     2221 gaagttcgag ggcgacaccc tggtgaaccg catcgagctg aagggcatcg acttcaagga
     2281 ggacggcaac atcctggggc acaagctgga gtacaactac aacagccaca aggtctatat
     2341 caccgccgac aagcagaaga acggcatcaa ggtgaacttc aagacccgcc acaacatcga
     2401 ggacggcagc gtgcagctcg ccgaccacta ccagcagaac acccccatcg gcgacggccc
     2461 cgtgctgctg cccgacaacc actacctgag cacccagtcc aagctgagca aagaccccaa
     2521 cgagaagcgc gatcacatgg tcctgctgga gttcgtgacc gccgccggga tcactctcgg
     2581 catggacgag ctgtacaagt aagcggccgc gactctagat cataatcagc cataccacat
     2641 t
//
