LOCUS       sequence-84421-e        1245 bp    DNA     linear   UNA 14-APR-2025
DEFINITION  Localization: Mitochondrial Membrane, Excitation: 487, Emission: 
            509.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1245)
  TITLE     Davidson mEmerald
REFERENCE   2  (bases 1 to 1245)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 14, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: mEmerald-TOMM20-N-10
FEATURES             Location/Qualifiers
     source          1..1245
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      17..26
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     regulatory      482..491
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(488..490,491..493,494..1207)
                     /codon_start=1
                     /product="brightly fluorescent monomeric variant of GFP"
                     /label=mEmerald
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHKVYITADKQKNGIK
                     VNFKTRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(533..554)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(794..813)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     1141..1162
                     /label=EGFP-C
                     /note="EGFP, forward primer"
ORIGIN
        1 cgtcagatcc gctagcgcca ccatggtggg tcggaacagc gccatcgccg ccggtgtatg
       61 cggggccctt ttcattgggt actgcatcta cttcgaccgc aaaagacgaa gtgaccccaa
      121 cttcaagaac aggcttcgag aacgaagaaa gaaacagaag cttgccaagg agagagctgg
      181 gctttccaag ttacctgacc ttaaagatgc tgaagctgtt cagaagttct tccttgaaga
      241 aatacagctt ggtgaagagt tactagctca aggtgaatat gagaagggcg tagaccatct
      301 gacaaatgca attgctgtgt gtggacagcc acagcagtta ctgcaggtct tacagcaaac
      361 tcttccacca ccagtgttcc agatgcttct gactaagctc ccaacaatta gtcagagaat
      421 tgtaagtgct cagagcttgg ctgaagatga tgtggaaggc ggtagcgggg atccaccggt
      481 cgccaccatg gtgagcaagg gcgaggagct gttcaccggg gtggtgccca tcctggtcga
      541 gctggacggc gacgtaaacg gccacaagtt cagcgtgtcc ggcgagggcg agggcgatgc
      601 cacctacggc aagctgaccc tgaagttcat ctgcaccacc ggcaagctgc ccgtgccctg
      661 gcccaccctc gtgaccacct tgacctacgg cgtgcagtgc ttcgcccgct accccgacca
      721 catgaagcag cacgacttct tcaagtccgc catgcccgaa ggctacgtcc aggagcgcac
      781 catcttcttc aaggacgacg gcaactacaa gacccgcgcc gaggtgaagt tcgagggcga
      841 caccctggtg aaccgcatcg agctgaaggg catcgacttc aaggaggacg gcaacatcct
      901 ggggcacaag ctggagtaca actacaacag ccacaaggtc tatatcaccg ccgacaagca
      961 gaagaacggc atcaaggtga acttcaagac ccgccacaac atcgaggacg gcagcgtgca
     1021 gctcgccgac cactaccagc agaacacccc catcggcgac ggccccgtgc tgctgcccga
     1081 caaccactac ctgagcaccc agtccaagct gagcaaagac cccaacgaga agcgcgatca
     1141 catggtcctg ctggagttcg tgaccgccgc cgggatcact ctcggcatgg acgagctgta
     1201 caagtaagcg gccgcgactc tagatcataa tcagccatac cacat
//
