LOCUS       sequence-84556-e         809 bp    DNA     linear   UNA 15-APR-2025
DEFINITION  Retroviral vector expresses hTIRAP-3xHA and a target site for the 
            biotin ligase BirA upstream of IRES and GFP.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 809)
  AUTHORS   Bonham KS, Orzalli MH, Hayashi K, Wolf AI, Glanemann C, Weninger W, 
            Iwasaki A, Knipe DM, Kagan JC
  TITLE     A promiscuous lipid-binding protein diversifies the subcellular 
            sites of toll-like receptor signal transduction.
  JOURNAL   Cell. 2014 Feb 13;156(4):705-16. doi: 10.1016/j.cell.2014.01.019.
  PUBMED    24529375
REFERENCE   2  (bases 1 to 809)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2014.01"; journalName: "Cell"; date: "2014-02-13- 
            13"; volume: "156"; issue: "4"; pages: "705-16"
COMMENT     Sequence Label: TIRAP-TAP_IRES_GFP
FEATURES             Location/Qualifiers
     source          1..809
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(112..156)
                     /codon_start=1
                     /product="peptide tag that allows for enzymatic 
                     biotinylation"
                     /label=AviTag(TM)
                     /translation="GLNDIFEAQKIEWHE"
     CDS             complement(166..210)
                     /codon_start=1
                     /product="peptide tag that allows for enzymatic 
                     biotinylation"
                     /label=AviTag(TM)
                     /translation="GLNDIFEAQKIEWHE"
     CDS             complement(214..234)
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     CDS             complement(250..270)
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     CDS             complement(286..306)
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     CDS             complement(307..387)
                     /codon_start=1
                     /product="three tandem HA epitope tags"
                     /label=3xHA
                     /translation="YPYDVPDYAYPYDVPDYAYPYDVPDYA"
ORIGIN
        1 acaacgcaca ccggccttat tccaagcggc ttcggccagt aacgttaggg gggggggcgg
       61 aattcgatat caagcttatc gataccgtcg acgtttaaac gcggccgcct attcgtgcca
      121 ttcgatcttc tgggcttcga agatgtcgtt gaggcctgcg gcagcttcat gccattcaat
      181 tttttgagct tcaaaaatat cattaagacc ccctccttgg aaatataggt tttccgttgt
      241 ggggatggcg ccctggaagt agaggttctc agtagtagga atagcacctt gaaaataaag
      301 attttccgca tagtctggta catcgtatgg ataggcgtag tcggggacgt cgtaggggta
      361 agcataatca ggaacatcgt aaggatactc gagagatcta agtagatcag atactgtagc
      421 tgaatcccgt tccccttctc cctcctgtag tagcttacaa cgcatgacag cttctttgac
      481 ttgacgaaag ccaccatcag ggcccctgcc atcgacgtag tacatgaatc ggagctcagg
      541 tgggtaggca gctctgctga ggcccgacag cagggggatg gtgcagccct cggcccctgg
      601 agcctcggtc agggcctgca gcatctggta cttgcaccag gggtcctgaa ggaagcccgg
      661 cgtgatgagc agcacccggc agtgactact gctcagtgcc tggcacagct cggacactat
      721 agcgccgcct ggggttgcat cccggagttg caggaagcag cgcaggctgg cagtgctgcc
      781 ttccaagtag gagaccaggt cctgggcgg
//
