LOCUS       sequence-84889-e         994 bp    DNA     linear   UNA 14-APR-2025
DEFINITION  Localization: Actin, Excitation: 400 / 504, Emission: 515 / 517.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 994)
  AUTHORS   Davidson Lab
  TITLE     Michael Davidson Empty Backbones
  JOURNAL   unpublished
REFERENCE   2  (bases 1 to 994)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 14, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "unpublished"
COMMENT     Sequence Label: PA-mCherry1-Lifeact-7
FEATURES             Location/Qualifiers
     source          1..994
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        48..99
                     /label=CMVd2 promoter
                     /note="truncated human cytomegalovirus (CMV) immediate 
                     early promoter that yields moderately reduced expression 
                     relative to the full-length promoter"
     primer_bind     50..70
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     regulatory      128..137
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             134..184
                     /codon_start=1
                     /product="peptide that binds filamentous actin (Riedl et 
                     al., 2008)"
                     /label=Lifeact
                     /note="first 17 amino acids of budding yeast Abp140"
                     /translation="MGVADLIKKFESISKEE"
     regulatory      200..209
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             206..916
                     /codon_start=1
                     /product="photoactivatable monomeric derivative of DsRed 
                     fluorescent protein (Subach et al., 2009)"
                     /label=PAmCherry1
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHVFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFTWDILSPQFMYGSNAYVKHPADIPDYFKLSFPEGFKWERVMKF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEALSERMYPEDGALK
                     GEVKPRVKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNRKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(351..369)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
ORIGIN
        1 aaaatcaacg ggactttcca aaatgtcgta acaactccgc ccccattgac gcaaatgggc
       61 ggtaggcgtg tacggtggga ggtctatata agcagagctg gtttagtgaa ccgtcagatc
      121 cgctagcgcc accatgggcg tggccgactt gatcaagaag ttcgagtcca tctccaagga
      181 ggagggggat ccaccggtcg ccaccatggt gagcaagggc gaggaggata acatggccat
      241 cattaaggag ttcatgcgct tcaaggtgca catggagggg tccgtgaacg gccacgtgtt
      301 cgagatcgag ggcgagggcg agggccgccc ctacgagggc acccagaccg ccaagctgaa
      361 ggtgaccaag ggcggccccc tgcccttcac ctgggacatc ctgagccctc agttcatgta
      421 cggctccaat gcctacgtga agcaccccgc cgacatcccc gactacttta agctgtcctt
      481 ccccgagggc ttcaagtggg agcgcgtgat gaaattcgag gacggcggcg tggtgaccgt
      541 gacccaggac tcctccctgc aggacggcga gttcatctac aaggtgaagc tgcgcggcac
      601 caacttcccc tccgacggcc ccgtgatgca gaagaagacc atgggctggg aggccctctc
      661 cgagcggatg taccccgagg acggcgccct gaagggcgag gtcaagccca gagtgaagct
      721 gaaggacggc ggccactacg acgctgaggt caagaccacc tacaaggcca agaagcccgt
      781 gcagctgccc ggcgcctaca acgtcaaccg caagctggac atcaccagcc acaacgagga
      841 ctacaccatc gtggagcagt acgagagagc cgagggccgc cactccaccg gcggcatgga
      901 cgagctgtac aagtaagcgg ccgcgactct agatcataat cagccatacc acatttgtag
      961 aggttttact tgctttaaaa aacctcccac acct
//
