LOCUS       sequence-8498-ex        3941 bp    DNA     circular SYN 14-APR-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3941)
  AUTHORS   Yu J, Vodyanik MA, Smuga-Otto K, Antosiewicz-Bourget J, Frane JL, 
            Tian S, Nie J, Jonsdottir GA, Ruotti V, Stewart R, Slukvin II, 
            Thomson JA
  TITLE     Induced pluripotent stem cell lines derived from human somatic 
            cells.
  JOURNAL   Science. 2007 Dec 21. 318(5858):1917-20.
  PUBMED    18029452
REFERENCE   2  (bases 1 to 3941)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 14, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Science. 
            2007 Dec 21. 318(5858):1917-20."
COMMENT     Sequence Label: pGEM-Nanog
FEATURES             Location/Qualifiers
     source          1..3941
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     regulatory      33..42
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     regulatory      37..46
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     promoter        complement(1066..1084)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(1067..1084)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(1102..1118)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1102..1118)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(1115..1137)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    1126..1142
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(1150..1156,1157..1174,1175..1180))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    1195..1216
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(1333..1350)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(1504..2092)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1584..1603)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(2263..3054,3055..3123))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     2886..2905
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(3124..3228)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(3306..3761)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(3443..3464)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     3655..3674
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     3887..3909
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     3901..3918
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     3902..3918
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     join(3925..3941,1..3)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        join(3925..3941,1..2)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 gggcgaattg ggcccgacgt cgcatgctcc cggccgccat ggcggccgcg ggaattcgat
       61 tcatcttcac acgtcttcag gttgcatgtt catggagtag tttaggaata aatccatggt
      121 ttgtggagta ctaaaatacc tagtggtctg ctgtattaca ttaaggcctt ccccagcagc
      181 ttccaaggca gcctccaagt cactggcagg agaatttggc tggaactgca tgcaggactg
      241 cagagattcc tctccacagt tatagaaggg actgttccag gcctgattgt tccaggattg
      301 ggtgcaccag gtctgagtgt tccaggagtg gttgctccag gactggatgt tctgggtctg
      361 gttgctccag gttgaattgt tccaggtctg gttgctccac attggaaggt tcccagtcgg
      421 gttcaccagg catccctggt ggtaggaaga gtaaaggctg gggtaggtag gtgctgaggc
      481 cttctgcgtc acaccattgc tattcttcgg ccagttgttt ttctgccacc tcttagattt
      541 cattctctgg ttctggaacc aggtcttcac ctgtttgtag ctgaggttca ggatgttgga
      601 gagttcttgc atctgctgga ggctgaggta tttctgtctc tgaaatctat cattgagtac
      661 acacagctgg gtggaagaga acacagttct ggtcttctgt ttcttgaccg ggaccttgtc
      721 ttcctttttt gcgacactct tctctgcaga agtgggttgt ttgcctttgg gactggtgga
      781 agaatcaggg ctgtcctgaa taagcagatc catggaggaa ggaagaggag agacagtctc
      841 cgtgtgaggc atctcagcag aagacatttg caaggatgga tagttttctt caggcccaca
      901 aatcacaggc ataggtgaag attctttaca gtcggatgct tcaaagcaag gcaagctttg
      961 gggacaagct ggatccacac tcatgaatca ctagtgaatt cgcggccgcc tgcaggtcga
     1021 ccatatggga gagctcccaa cgcgttggat gcatagcttg agtattctat agtgtcacct
     1081 aaatagcttg gcgtaatcat ggtcatagct gtttcctgtg tgaaattgtt atccgctcac
     1141 aattccacac aacatacgag ccggaagcat aaagtgtaaa gcctggggtg cctaatgagt
     1201 gagctaactc acattaattg cgttgcgctc actgcccgct ttccagtcgg gaaacctgtc
     1261 gtgccagctg cattaatgaa tcggccaacg cgcggggaga ggcggtttgc gtattgggcg
     1321 ctcttccgct tcctcgctca ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt
     1381 atcagctcac tcaaaggcgg taatacggtt atccacagaa tcaggggata acgcaggaaa
     1441 gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc
     1501 gtttttccat aggctccgcc cccctgacga gcatcacaaa aatcgacgct caagtcagag
     1561 gtggcgaaac ccgacaggac tataaagata ccaggcgttt ccccctggaa gctccctcgt
     1621 gcgctctcct gttccgaccc tgccgcttac cggatacctg tccgcctttc tcccttcggg
     1681 aagcgtggcg ctttctcata gctcacgctg taggtatctc agttcggtgt aggtcgttcg
     1741 ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg ccttatccgg
     1801 taactatcgt cttgagtcca acccggtaag acacgactta tcgccactgg cagcagccac
     1861 tggtaacagg attagcagag cgaggtatgt aggcggtgct acagagttct tgaagtggtg
     1921 gcctaactac ggctacacta gaagaacagt atttggtatc tgcgctctgc tgaagccagt
     1981 taccttcgga aaaagagttg gtagctcttg atccggcaaa caaaccaccg ctggtagcgg
     2041 tggttttttt gtttgcaagc agcagattac gcgcagaaaa aaaggatctc aagaagatcc
     2101 tttgatcttt tctacggggt ctgacgctca gtggaacgaa aactcacgtt aagggatttt
     2161 ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa aatgaagttt
     2221 taaatcaatc taaagtatat atgagtaaac ttggtctgac agttaccaat gcttaatcag
     2281 tgaggcacct atctcagcga tctgtctatt tcgttcatcc atagttgcct gactccccgt
     2341 cgtgtagata actacgatac gggagggctt accatctggc cccagtgctg caatgatacc
     2401 gcgagaccca cgctcaccgg ctccagattt atcagcaata aaccagccag ccggaagggc
     2461 cgagcgcaga agtggtcctg caactttatc cgcctccatc cagtctatta attgttgccg
     2521 ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg ccattgctac
     2581 aggcatcgtg gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg gttcccaacg
     2641 atcaaggcga gttacatgat cccccatgtt gtgcaaaaaa gcggttagct ccttcggtcc
     2701 tccgatcgtt gtcagaagta agttggccgc agtgttatca ctcatggtta tggcagcact
     2761 gcataattct cttactgtca tgccatccgt aagatgcttt tctgtgactg gtgagtactc
     2821 aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc cggcgtcaat
     2881 acgggataat accgcgccac atagcagaac tttaaaagtg ctcatcattg gaaaacgttc
     2941 ttcggggcga aaactctcaa ggatcttacc gctgttgaga tccagttcga tgtaacccac
     3001 tcgtgcaccc aactgatctt cagcatcttt tactttcacc agcgtttctg ggtgagcaaa
     3061 aacaggaagg caaaatgccg caaaaaaggg aataagggcg acacggaaat gttgaatact
     3121 catactcttc ctttttcaat attattgaag catttatcag ggttattgtc tcatgagcgg
     3181 atacatattt gaatgtattt agaaaaataa acaaataggg gttccgcgca catttccccg
     3241 aaaagtgcca cctgatgcgg tgtgaaatac cgcacagatg cgtaaggaga aaataccgca
     3301 tcaggaaatt gtaagcgtta atattttgtt aaaattcgcg ttaaattttt gttaaatcag
     3361 ctcatttttt aaccaatagg ccgaaatcgg caaaatccct tataaatcaa aagaatagac
     3421 cgagataggg ttgagtgttg ttccagtttg gaacaagagt ccactattaa agaacgtgga
     3481 ctccaacgtc aaagggcgaa aaaccgtcta tcagggcgat ggcccactac gtgaaccatc
     3541 accctaatca agttttttgg ggtcgaggtg ccgtaaagca ctaaatcgga accctaaagg
     3601 gagcccccga tttagagctt gacggggaaa gccggcgaac gtggcgagaa aggaagggaa
     3661 gaaagcgaaa ggagcgggcg ctagggcgct ggcaagtgta gcggtcacgc tgcgcgtaac
     3721 caccacaccc gccgcgctta atgcgccgct acagggcgcg tccattcgcc attcaggctg
     3781 cgcaactgtt gggaagggcg atcggtgcgg gcctcttcgc tattacgcca gctggcgaaa
     3841 gggggatgtg ctgcaaggcg attaagttgg gtaacgccag ggttttccca gtcacgacgt
     3901 tgtaaaacga cggccagtga attgtaatac gactcactat a
//
