LOCUS       sequence-85008-e         938 bp    DNA     linear   UNA 14-APR-2025
DEFINITION  Localization: C1 Cloning Vector, Excitation: 558, Emission: 583.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 938)
  AUTHORS   Davidson Lab
  TITLE     Michael Davidson Empty Backbones
  JOURNAL   unpublished
REFERENCE   2  (bases 1 to 938)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 14, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "unpublished"
COMMENT     Sequence Label: DsRed2-C1
FEATURES             Location/Qualifiers
     source          1..938
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        2..40
                     /label=minimal CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     regulatory      79..88
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             85..759
                     /codon_start=1
                     /product="improved tetrameric variant of DsRed fluorescent 
                     protein"
                     /label=DsRed2
                     /note="mammalian codon-optimized"
                     /translation="MASSENVITEFMRFKVRMEGTVNGHEFEIEGEGEGRPYEGHNTVK
                     LKVTKGGPLPFAWDILSPQFQYGSKVYVKHPADIPDYKKLSFPEGFKWERVMNFEDGGV
                     ATVTQDSSLQDGCFIYKVKFIGVNFPSDGPVMQKKTMGWEASTERLYPRDGVLKGETHK
                     ALKLKDGGHYLVEFKSIYMAKKPVQLPGYYYVDAKLDITSHNEDYTIVEQYERTEGRHH
                     LFL"
     primer_bind     complement(265..285)
                     /label=DsRed1-N
                     /note="DsRed1, reverse primer"
     primer_bind     677..700
                     /label=DsRed1-C
                     /note="DsRed1, forward primer"
     misc_feature    760..825
                     /label=MCS
                     /note="multiple cloning site"
ORIGIN
        1 cggtaggcgt gtacggtggg aggtctatat aagcagagct ggtttagtga accgtcagat
       61 ccgctagcgc taccggtcgc caccatggcc tcctccgaga acgtcatcac cgagttcatg
      121 cgcttcaagg tgcgcatgga gggcaccgtg aacggccacg agttcgagat cgagggcgag
      181 ggcgagggcc gcccctacga gggccacaac accgtgaagc tgaaggtgac caagggcggc
      241 cccctgccct tcgcctggga catcctgtcc ccccagttcc agtacggctc caaggtgtac
      301 gtgaagcacc ccgccgacat ccccgactac aagaagctgt ccttccccga gggcttcaag
      361 tgggagcgcg tgatgaactt cgaggacggc ggcgtggcga ccgtgaccca ggactcctcc
      421 ctgcaggacg gctgcttcat ctacaaggtg aagttcatcg gcgtgaactt cccctccgac
      481 ggccccgtga tgcagaagaa gaccatgggc tgggaggcct ccaccgagcg cctgtacccc
      541 cgcgacggcg tgctgaaggg cgagacccac aaggccctga agctgaagga cggcggccac
      601 tacctggtgg agttcaagtc catctacatg gccaagaagc ccgtgcagct gcccggctac
      661 tactacgtgg acgccaagct ggacatcacc tcccacaacg aggactacac catcgtggag
      721 cagtacgagc gcaccgaggg ccgccaccac ctgttcctgt ccggactcag atctcgagct
      781 caagcttcga attctgcagt cgacggtacc gcgggcccgg gatccaccgg atctagataa
      841 ctgatcataa tcagcataca catttgtaga ggtttacttg cttaaaaacc tccacactcc
      901 cctgacctga acataaatga tgcattgtgt gtactgtt
//
