LOCUS       sequence-85225-e        1291 bp    DNA     linear   UNA 03-SEP-2025
DEFINITION  Localization: Protein Expression Vector , Excitation: 530, Emission:
            541.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1291)
  AUTHORS   Hoi H, Howe ES, Ding Y, Zhang W, Baird MA, Sell BR, Allen JR, 
            Davidson MW, Campbell RE
  TITLE     An engineered monomeric Zoanthus sp. yellow fluorescent protein.
  JOURNAL   Chem Biol. 2013 Oct 24;20(10):1296-304. doi: 
            10.1016/j.chembiol.2013.08.008. Epub 2013 Oct 3.
  PUBMED    24094838
REFERENCE   2  (bases 1 to 1291)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Sep 3, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.chembiol.2013.08.008"; journalName: "Chem Biol"; date: 
            "2013-10-24- 24"; volume: "20"; issue: "10"; pages: "1296-304"
COMMENT     Sequence Label: mPapaya-pBAD
FEATURES             Location/Qualifiers
     source          1..1291
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             join(17..25,26..40)
                     /codon_start=1
                     /product="Xpress(TM) epitope tag, including an enterokinase
                     recognition and cleavage site"
                     /label=Xpress(TM) tag
                     /translation="DLYDDDDK"
     CDS             56..769
                     /codon_start=1
                     /product="monomeric yellow fluorescent protein derived from
                     the Zoanthus fluorescent protein zFP538"
                     /label=mPapaya1
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEGQSKHGLKEEMTVKYHMEGCVNGHKFVITGEGIGNPFKG
                     KQTANLCVIEGGPLPFSEDILSPGFKYGDRIFTEYPQDIVDYFKNSCPAGYTWERSFLF
                     EDGAVCRCNVDITVSEKENCIYHKSIFRGVNFPADGPVMKKMTTNWEASTEKIVPVPKQ
                     GILKGKVKMYLLLKDGGRYHCQFDTVYKAKSVPSKMPEWHFIQHKLLREDRSDAKNQKW
                     QLTEHAIAGMDELYK"
     primer_bind     complement(815..832)
                     /label=pBAD Reverse
                     /note="For vectors with E. coli araBAD promoter, reverse 
                     primer"
     terminator      985..1070
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
ORIGIN
        1 gtgcacaatg ggtcgggatc tgtacgacga tgacgataag gatccgagct cgagcatggt
       61 gagcaagggc gaggggcaat cgaagcatgg gctaaaggag gagatgacgg tgaagtacca
      121 catggaaggt tgcgttaatg gtcacaaatt tgtcattact ggagagggca ttggaaaccc
      181 ttttaagggt aagcaaactg caaatttgtg tgtgatagaa ggaggcccgc tgccgttctc
      241 ggaggacatt ttaagtccgg gttttaaata tggtgaccgg attttcacag agtacccaca
      301 ggatattgta gattacttca agaactcatg tccagcgggc tatacgtggg aaaggagctt
      361 cctctttgag gacggagcgg tctgtcgatg caacgtggac ataacagtct ctgaaaagga
      421 gaactgcatc tatcacaaaa gtatcttcag aggggtgaat tttcccgccg acggccccgt
      481 aatgaagaaa atgaccacta attgggaagc tagtaccgag aaaattgtgc ctgttccaaa
      541 gcaagggata ttaaaaggaa aggtcaaaat gtacctattg ctgaaggatg gcggtcgtta
      601 tcattgccag tttgatacgg tatataaagc taaatcagtg ccctccaaga tgccagaatg
      661 gcattttata cagcataagc tccttaggga ggaccgctct gatgctaaaa accagaaatg
      721 gcaattaaca gaacatgcaa tcgcaggcat ggacgagctg tacaagtaag aattcgaagc
      781 ttggctgttt tggcggatga gagaagattt tcagcctgat acagattaaa tcagaacgca
      841 gaagcggtct gataaaacag aatttgcctg gcggcagtag cgcggtggtc ccacctgacc
      901 ccatgccgaa ctcagaagtg aaacgccgta gcgccgatgg tagtgtgggg tctccccatg
      961 cgagagtagg gaactgccag gcatcaaata aaacgaaagg ctcagtcgaa agactgggcc
     1021 tttcgtttta tctgttgttt gtcggtgacg ctctcctgag taggacaaat cgcgggagcg
     1081 atttgacgtg cgagcacgcg cgcgggagag gggtggcggc cagacgcccc gccataactg
     1141 ccaggcatca aaatttagca gaagcattct gacgatgctt ttggcgattc tacactccat
     1201 gtaattccaa atcgttccaa tgtatcggct ctagtgaaca taccctgata caatggcctc
     1261 tccattatat atatgtgaaa aagcgcagcg t
//
