LOCUS       sequence-85243-e        1052 bp    DNA     linear   UNA 14-APR-2025
DEFINITION  Expression of human MICU1 with Flag tag in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1052)
  AUTHORS   Sancak Y, Markhard AL, Kitami T, Kovacs-Bogdan E, Kamer KJ, Udeshi 
            ND, Carr SA, Chaudhuri D, Clapham DE, Li AA, Calvo SE, Goldberger O,
            Mootha VK
  TITLE     EMRE is an Essential Component of the Mitochondrial Calcium 
            Uniporter Complex.
  JOURNAL   Science. 2013 Nov 14.
  PUBMED    24231807
REFERENCE   2  (bases 1 to 1052)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 14, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Science. 
            2013 Nov 14."
COMMENT     Sequence Label: pLYS1-MICU1-Flag
FEATURES             Location/Qualifiers
     source          1..1052
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(14..37)
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
ORIGIN
        1 attcggtacc ttacttgtca tcgtcatcct tgtaatccat agatccacca gatccaccag
       61 atccaccccc gggctgtttg ggtaaagcga agtcccaggc agtttcctgt gcacatttcc
      121 acatggcctg catgaggcga gtgaaaccca tgtctttggg cttttccagg cctctcatca
      181 gccgttgctt catgatggaa acaaattctt tgttactgag ctctccgttt ccatcacagt
      241 caaagagtgc aaacaccaca tcacacacgt ggtctgagag ctccacttta gccactgtcc
      301 tggccacctg ctgcatggtc actttatcaa gagatgctcc agccatatgg taaaaactca
      361 atgcagtgtc cacatcatta atgttcttta ggaaagtaaa gaagttctcc acctcctgaa
      421 atgtcagacc ctttccttct ttgaagtgct tcttgagctg cctctgcatg gcggtcagct
      481 tcttggactg caccccactg taggcaagta gcatgccacc aaactgcctc tcagtaattc
      541 tcccatccac agggtcatgg cgttcaaact caagcttcag aacatcatgc tgcagtttac
      601 gctgaaattc gaggaagttt ttgattgtca gctttccctt cagatcagct ccaaaaaagt
      661 aggttgtgag ggctgaacac aagccagact tgagggtgtt gccagtagtt ggacgatctc
      721 tgtggcgcat acccatactg gtttgggagc gaatgatgct ctgaacctgt tcaaattctt
      781 ccatatctac ttctccatct ccattcaaat caaacatctt gaaggcaatt tcaaaatttc
      841 tctgaggagt ggaaagaaca gttgtgagga aaatgtagtc tgaaaaggat atgagcccac
      901 attctccaag ggtgtaaaat atactgcctt catcagcaaa tttttctcgt tcctgggaaa
      961 ttttctttcc atcaaagcgt tttattatat attgatccag acccaagtgt tctggttgtt
     1021 tttcattggg tgttatggat cgcacaaaat ct
//
