LOCUS       sequence-85380-e        1763 bp    DNA     linear   UNA 14-APR-2025
DEFINITION  Localization: Membrane Receptor, Excitation: 587, Emission: 610.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1763)
  TITLE     Davidson lab - mCherry constructs
REFERENCE   2  (bases 1 to 1763)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 14, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: mCherry-ATG3-C-18
FEATURES             Location/Qualifiers
     source          1..1763
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      17..26
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             23..730
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(168..186)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     436..455
                     /label=mCherry-F
                     /note="mCherry, forward primer"
ORIGIN
        1 gctagcgcta ccggtcgcca ccatggtgag caagggcgag gaggataaca tggccatcat
       61 caaggagttc atgcgcttca aggtgcacat ggagggctcc gtgaacggcc acgagttcga
      121 gatcgagggc gagggcgagg gccgccccta cgagggcacc cagaccgcca agctgaaggt
      181 gaccaagggt ggccccctgc ccttcgcctg ggacatcctg tcccctcagt tcatgtacgg
      241 ctccaaggcc tacgtgaagc accccgccga catccccgac tacttgaagc tgtccttccc
      301 cgagggcttc aagtgggagc gcgtgatgaa cttcgaggac ggcggcgtgg tgaccgtgac
      361 ccaggactcc tccctgcagg acggcgagtt catctacaag gtgaagctgc gcggcaccaa
      421 cttcccctcc gacggccccg taatgcagaa gaagaccatg ggctgggagg cctcctccga
      481 gcggatgtac cccgaggacg gcgccctgaa gggcgagatc aagcagaggc tgaagctgaa
      541 ggacggcggc cactacgacg ctgaggtcaa gaccacctac aaggccaaga agcccgtgca
      601 gctgcccggc gcctacaacg tcaacatcaa gttggacatc acctcccaca acgaggacta
      661 caccatcgtg gaacagtacg aacgcgccga gggccgccac tccaccggcg gcatggacga
      721 gctgtacaag tccggactca gatctagtag cggaggtagc gcatccggcg gaagcggagg
      781 aagccagaat gtgatcaaca cggtgaaggg aaaggctctg gaagtggccg agtacctgac
      841 cccggtcctc aaggaatcaa aatttaagga aacgggtgta atcactccag aagagtttgt
      901 ggcagctgga gatcacttag tccaccactg tccaacatgg caatgggcta caggggaaga
      961 attgaaagtg aaggcatatc ttccgacaga caaacaattt ttggtaacca aaaatgttcc
     1021 atgctacaag cggtgtaaac agatggagta ttcggatgaa ttggaagcta tcattgaaga
     1081 agatgatggt gatgggggat gggtagatac atatcacaac acaggtatta caggaattac
     1141 tgaagcagtt aaggagatta cactggaaag caaggacagt ataaaactcc aagattgctc
     1201 agcactgtgt gatgaagaag acgaggaaga tgaaggggaa gctgcagaca tggaagaata
     1261 tgaagagagt ggattgttgg aaacagatga ggctacccta gacacaagga aaatagtgga
     1321 agcctgcaaa gctaaggctg acgctggagg tgaagatgct attttacaaa cgagaacata
     1381 cgatctgtac atcacttacg acaaatatta ccagacacca cggctatggt tgtttggcta
     1441 tgatgagcaa cggcagcctt taacagttga gcacatgtat gaagacatca gtcaagatca
     1501 tgtgaagaaa acagtgacca ttgaaaacca tcctcatctc ccaccacctc ctatgtgttc
     1561 agttcaccca tgcaggcatg ctgaagtgat gaagaaaatt attgagacag ttgcagaagg
     1621 cgggggagag cttggtgttc atatgtatct tttaattttt ttgaaatttg ttcaagctgt
     1681 cattccaaca atagaatatg actacacaag acacttcaca atgtagggat ccaccggatc
     1741 tagataactg atcataatca gcc
//
