LOCUS       sequence-85381-e        1769 bp    DNA     linear   UNA 14-APR-2025
DEFINITION  Localization: Membrane Receptor, Excitation: 587, Emission: 610.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1769)
  TITLE     Davidson lab - mCherry constructs
REFERENCE   2  (bases 1 to 1769)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 14, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: mCherry-ATG3-N-18
FEATURES             Location/Qualifiers
     source          1..1769
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      1017..1026
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             1023..1733
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(1168..1186)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     1436..1455
                     /label=mCherry-F
                     /note="mCherry, forward primer"
ORIGIN
        1 atccgctagc gctaccggac tcagatctcg aggccaccat gcagaatgtg atcaacacgg
       61 tgaagggaaa ggctctggaa gtggccgagt acctgacccc ggtcctcaag gaatcaaaat
      121 ttaaggaaac gggtgtaatc actccagaag agtttgtggc agctggagat cacttagtcc
      181 accactgtcc aacatggcaa tgggctacag gggaagaatt gaaagtgaag gcatatcttc
      241 cgacagacaa acaatttttg gtaaccaaaa atgttccatg ctacaagcgg tgtaaacaga
      301 tggagtattc ggatgaattg gaagctatca ttgaagaaga tgatggtgat gggggatggg
      361 tagatacata tcacaacaca ggtattacag gaattactga agcagttaag gagattacac
      421 tggaaagcaa ggacagtata aaactccaag attgctcagc actgtgtgat gaagaagacg
      481 aggaagatga aggggaagct gcagacatgg aagaatatga agagagtgga ttgttggaaa
      541 cagatgaggc taccctagac acaaggaaaa tagtggaagc ctgcaaagct aaggctgacg
      601 ctggaggtga agatgctatt ttacaaacga gaacatacga tctgtacatc acttacgaca
      661 aatattacca gacaccacgg ctatggttgt ttggctatga tgagcaacgg cagcctttaa
      721 cagttgagca catgtatgaa gacatcagtc aagatcatgt gaagaaaaca gtgaccattg
      781 aaaaccatcc tcatctccca ccacctccta tgtgttcagt tcacccatgc aggcatgctg
      841 aagtgatgaa gaaaattatt gagacagttg cagaaggcgg gggagagctt ggtgttcata
      901 tgtatctttt aatttttttg aaatttgttc aagctgtcat tccaacaata gaatatgact
      961 acacaagaca cttcacaatg ggcagcgcat ccggcggaag cggaagcgga ccggtcgcca
     1021 ccatggtgag caagggcgag gaggataaca tggccatcat caaggagttc atgcgcttca
     1081 aggtgcacat ggagggctcc gtgaacggcc acgagttcga gatcgagggc gagggcgagg
     1141 gccgccccta cgagggcacc cagaccgcca agctgaaggt gaccaagggt ggccccctgc
     1201 ccttcgcctg ggacatcctg tcccctcagt tcatgtacgg ctccaaggcc tacgtgaagc
     1261 accccgccga catccccgac tacttgaagc tgtccttccc cgagggcttc aagtgggagc
     1321 gcgtgatgaa cttcgaggac ggcggcgtgg tgaccgtgac ccaggactcc tccctgcagg
     1381 acggcgagtt catctacaag gtgaagctgc gcggcaccaa cttcccctcc gacggccccg
     1441 taatgcagaa gaagaccatg ggctgggagg cctcctccga gcggatgtac cccgaggacg
     1501 gcgccctgaa gggcgagatc aagcagaggc tgaagctgaa ggacggcggc cactacgacg
     1561 ctgaggtcaa gaccacctac aaggccaaga agcccgtgca gctgcccggc gcctacaacg
     1621 tcaacatcaa gttggacatc acctcccaca acgaggacta caccatcgtg gaacagtacg
     1681 aacgcgccga gggccgccac tccaccggcg gcatggacga gctgtacaag taaagcggcc
     1741 gcgactctag atcataatca gccatacca
//
