LOCUS       sequence-85394-e        1829 bp    DNA     linear   UNA 14-APR-2025
DEFINITION  Localization: Lysosomes, Excitation: 587, Emission: 610.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1829)
  TITLE     Davidson lab - mCherry constructs
REFERENCE   2  (bases 1 to 1829)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 14, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: mCherry-CathespinB-6
FEATURES             Location/Qualifiers
     source          1..1829
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      1073..1082
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             1079..1789
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(1224..1242)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     1492..1511
                     /label=mCherry-F
                     /note="mCherry, forward primer"
ORIGIN
        1 tgacgtcaga tccgctagcg ctaccggact cagatctcga gctcaagctt cgaattccag
       61 gatgtggtgg tccttgatcc ctctctcttg cctgctggca ctgaccagtg cccatgacaa
      121 gccttccttt cacccactgt cggacgacat gattaactat atcaacaaac agaatacaac
      181 atggcaggct ggacgcaact tctacaatgt tgacataagc tatctgaaga agctgtgtgg
      241 aactgtcctg ggtggaccca agctgccgga aagggttggg ttcagcgagg acataaatct
      301 acctgaatcc tttgatgcac gggaacagtg gtccaattgc ccgaccatcg cacagatcag
      361 agaccagggg tcctgtggct cttgttgggc atttggggca gtggaagcca tgtctgaccg
      421 aatctgcatt cacaccaatg gccgagtcaa tgtggaggtg tctgctgagg acctgcttac
      481 ctgctgtggt atccagtgtg gggatggctg taatggtggc tatccctctg gagcatggaa
      541 cttctggact agaaaaggcc tggtttctgg tggagtatac aattctcata taggctgctt
      601 accctacacc atccctccct gtgaacacca tgtcaatggc tcccgtcccc catgcactgg
      661 agaaggagat actcccaagt gcaacaagat gtgtgaggct ggctactcca catcctacaa
      721 ggaagataag cactatgggt acacttccta cagtgtgtct gacagcgaga aggagatcat
      781 ggcggaaatc tacaaaaatg gcccagtgga gggtgctttt actgtgtttt ctgacttctt
      841 gacttacaaa tcaggcgtat acaagcatga agccggtgat gtgatgggag gccatgccat
      901 ccgcattctg ggctggggaa tagagaatgg agtaccctac tggctggtag caaactcctg
      961 gaacgttgac tggggtgata atggtttctt taaaatcctc agaggagaga accactgtgg
     1021 aattgaatca gaaatcgtgg ctggaatccc acgcactcag gatccaccgg tcgccaccat
     1081 ggtgagcaag ggcgaggagg ataacatggc catcatcaag gagttcatgc gcttcaaggt
     1141 gcacatggag ggctccgtga acggccacga gttcgagatc gagggcgagg gcgagggccg
     1201 cccctacgag ggcacccaga ccgccaagct gaaggtgacc aagggtggcc ccctgccctt
     1261 cgcctgggac atcctgtccc ctcagttcat gtacggctcc aaggcctacg tgaagcaccc
     1321 cgccgacatc cccgactact tgaagctgtc cttccccgag ggcttcaagt gggagcgcgt
     1381 gatgaacttc gaggacggcg gcgtggtgac cgtgacccag gactcctccc tgcaggacgg
     1441 cgagttcatc tacaaggtga agctgcgcgg caccaacttc ccctccgacg gccccgtaat
     1501 gcagaagaag accatgggct gggaggcctc ctccgagcgg atgtaccccg aggacggcgc
     1561 cctgaagggc gagatcaagc agaggctgaa gctgaaggac ggcggccact acgacgctga
     1621 ggtcaagacc acctacaagg ccaagaagcc cgtgcagctg cccggcgcct acaacgtcaa
     1681 catcaagttg gacatcacct cccacaacga ggactacacc atcgtggaac agtacgaacg
     1741 cgccgagggc cgccactcca ccggcggcat ggacgagctg tacaagtaaa gcggccgcga
     1801 ctctagatca taatcagcca taccacatt
//
