LOCUS       sequence-85417-e        4647 bp    DNA     linear   UNA 15-APR-2025
DEFINITION  Localization: Dynein Receptor, Excitation: 587, Emission: 610.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 4647)
  TITLE     Davidson lab - mCherry constructs
REFERENCE   2  (bases 1 to 4647)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: mCherry-Dynactin-C-18
FEATURES             Location/Qualifiers
     source          1..4647
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      31..40
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             37..744
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(182..200)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     450..469
                     /label=mCherry-F
                     /note="mCherry, forward primer"
ORIGIN
        1 gtgacgtcag atccgctagc gctaccggtc gccaccatgg tgagcaaggg cgaggaggat
       61 aacatggcca tcatcaagga gttcatgcgc ttcaaggtgc acatggaggg ctccgtgaac
      121 ggccacgagt tcgagatcga gggcgagggc gagggccgcc cctacgaggg cacccagacc
      181 gccaagctga aggtgaccaa gggtggcccc ctgcccttcg cctgggacat cctgtcccct
      241 cagttcatgt acggctccaa ggcctacgtg aagcaccccg ccgacatccc cgactacttg
      301 aagctgtcct tccccgaggg cttcaagtgg gagcgcgtga tgaacttcga ggacggcggc
      361 gtggtgaccg tgacccagga ctcctccctg caggacggcg agttcatcta caaggtgaag
      421 ctgcgcggca ccaacttccc ctccgacggc cccgtaatgc agaagaagac catgggctgg
      481 gaggcctcct ccgagcggat gtaccccgag gacggcgccc tgaagggcga gatcaagcag
      541 aggctgaagc tgaaggacgg cggccactac gacgctgagg tcaagaccac ctacaaggcc
      601 aagaagcccg tgcagctgcc cggcgcctac aacgtcaaca tcaagttgga catcacctcc
      661 cacaacgagg actacaccat cgtggaacag tacgaacgcg ccgagggccg ccactccacc
      721 ggcggcatgg acgagctgta caagtccgga ctcagatctc gagagtagcg gaggtagcgc
      781 atccggcgga agcggaggag tacggaggca agcgcccggc ccctgcgggt tggctcccgc
      841 gtggaggtga ttgggaaggg ccaccgaggc actgtggcct atgttggagc cacactcttt
      901 gccactggca aatgggtggg cgtgattctg gatgaagcaa aaggcaaaaa tgatggcact
      961 gtccagggaa ggaagtattt cacatgtgat gaaggccacg gcatctttgt acgccagtcc
     1021 cagatccaag tatttgaaga tggagcagat actacttccc tagagactcc tgattcttct
     1081 gcttcaaagg tcctcaagag agagggagcc gatgcagctg caaagaccag caaactgcgg
     1141 ggactgaagc ctaagaaggc accgacagcc cgaaagacca caactcgacg gcccaagcct
     1201 actcgcccag ccagcactgg ggtggctggg cccagtagct cccttggccc ctctggctca
     1261 gcgtcagccg gggaactaag cagcagtgag cccagcaccc cagctcagac tccgctggca
     1321 gcacccatca tccccacacc ggccctcacc tctcctggag cagcaccccc acttccatct
     1381 ccctctaagg aagaggaagg gctgagggct caggtacggg acctggagga gaagctggag
     1441 accctgcgcc taaaacgctc agaagacaaa gcaaagctga aagagctgga gaagcacaag
     1501 atccagctgg agcaggtgca ggaatggaag agcaaaatgc aggagcagca ggcagacctg
     1561 cagcggcgcc tcaaggaggc tcggaaggaa gccaaggagg cgctagaggc aaaggaacgc
     1621 tacatggagg agatggccga cacagccgac gctatcgaga tggccactct ggacaaggag
     1681 atggctgaag agcgcgctga gtctctgcag caagaggtgg aggcactgaa ggaacgggta
     1741 gacgagctca ccacagacct ggagattctc aaggctgaaa tcgaagagaa aggctctgat
     1801 ggggccgcat caagctacca gctcaagcag ctggaggagc agaatgcccg cctgaaggat
     1861 gccctggtga ggatgcgaga cctctcttcc tcagagaagc aggagcacgt gaagctgcag
     1921 aaactcatgg aaaagaaaaa ccaggagctg gaggtcgtgc ggcagcagcg cgagcgtctt
     1981 caggaggagc tgagccaggc tgagagcacc atcgatgagc tcaaagagca ggtggacgcc
     2041 gctctgggag ccgaggagat ggtggagatg ctgaccgacc ggaacctgaa tctagaggag
     2101 aaagtgcggg agttacggga gactgtgggg gacttggaag ccatgaacga gatgaacgat
     2161 gagctgcagg agaacgcacg ggagacggag ctggaactcc gagagcagct ggacatggcg
     2221 ggcgcccgag tgagggaagc gcagaagcga gtggaagccg cccaggagac agtcgccgac
     2281 taccagcaga ccatcaagaa gtaccgccag ttgactgccc acctacagga tgtcaatcgg
     2341 gagctgacaa accagcagga agcgtctgta gagaggcagc agcagccgcc gccagagact
     2401 tttgatttca aaatcaagtt tgctgagacc aaggctcatg ccaaggccat tgagatggag
     2461 ttgagacaga tggaagttgc ccaggccaac cggcacatgt ccctgctgac agcctttatg
     2521 cctgacagct tccttcggcc aggtggagac cacgactgtg tcctggtgct gctgctcatg
     2581 ccccgactca tttgcaaggc agagctcatc cggaagcagg cccaggagaa gtttgacctg
     2641 agcgagaact gttcggagcg gcccgggctg cggggagctg ccggggagca gctgagcttt
     2701 gctgctggac tggtgtactc gctgagtctg ctgcaggcca cgctgcaccg ctatgagcat
     2761 gccctctctc agtgcagtgt ggacgtgtat aagaaggtcg gcagcctgta ccccgagatg
     2821 agcgcccacg agcgctcctt agatttcctc attgagctgc tgcacaagga tcagctggat
     2881 gagactgtca acgtggagcc cctcaccaag gccatcaagt attaccagca tctgtacagc
     2941 atccacctcg ctgaacaacc cgaggactcc accatgcagc tggctgacca catcaagttc
     3001 acccagagtg ccctggactg catgggcgtg gaggtggggc ggctgcgtgc cttcttgcag
     3061 ggtgggcagg aggcaacaga tattgccctt cttctccgag acctggaaac atcatgtagt
     3121 gacacccgtc agttctgcaa gaagatccga aggcggatgc cggggacgga tgctcctggg
     3181 atcccagcag cgctggcctt tggctcacag gtatccgaca cactcctgga ctgcaggaag
     3241 cacttgacgt gggtggtagc tgttctgcag gaggtggcag ctgcagccgc ccagcttatt
     3301 gcccccttgg cagagaacga ggggctgcct gtggctgcac tggaggagct ggccttcaaa
     3361 gcaagcgagc agatctacgg gagcccctcc agcagcccct atgagtgtct acgccagtca
     3421 tgcaccatcc tcatcagcac gatgaacaag ctggccacag ccatgcaaga aggcgagtat
     3481 gacgcagagc gacccccgag caagcctcct ccggttgaac ttcgggctgc agccctgcgt
     3541 gcagagatca cagatgctga aggtctgggt ttgaagcttg aggatcgaga gacagttatc
     3601 aaggagttaa agaagtcact caagattaag ggagaggagc tgagtgaggc caacgtgcgg
     3661 ctcagcctcc tggagaagaa gttggacagc gctgccaagg atgcagacga gcgaatcgag
     3721 aaagttcaga cacggctgga cgagactcag accctgctgc ggaagaagga gaaagacttt
     3781 gaggagacaa tggacgcact ccaggctgac atcgaccagc tggaggcaga gaaggcagag
     3841 ctcaagcagc gcctgaacag ccagtccaag cgcacaatcg aggggctccg gggcccccct
     3901 ccgtcaggca tcgctaccct ggtctctggc atcgctggtg aggaaccaca gcgagggggc
     3961 gctcctgggc aggctccggg cgccttgcca ggcccggggc tggtgaagga ctccccactg
     4021 ctgcttcagc agatctctgc tatgaggcta cacatctctc agctccagca tgagaacagc
     4081 atcctcagag gagcccagat gaaggcgtcc ttggcagctc tgccccctct gcatgttgca
     4141 aagctttccc tcccacccca tgagggcccc ggtggtaacc tagtggctgg ggcactgtac
     4201 cgcaagacca gccagctcct ggagaaacta aaccagctga gtacccacac ccacgtggtg
     4261 gatatcactc ggagcagccc agctgccaag agcccgtcag ctcagcttat ggaacaagtg
     4321 gctcagctca agtccctgag tgacaccatt gagaagctca aggatgaggt cctcaaggag
     4381 acagtgactc agcgccctgg agccactgtg cccaccgact ttgccacttt cccttcatct
     4441 gccttcctca gggccaagga agagcagcaa gatgacacag tctacatggg caaagtgacc
     4501 ttttcatgcg cggcaggcct aggacagcga caccgcctgg tgctgaccca ggagcagctg
     4561 caccagcttc acagtcgcct catctcctaa ggtaccgcgg gcccgggatc caccggatct
     4621 agataactga tcataatcag ccatacc
//
