LOCUS       sequence-85444-e        1154 bp    DNA     linear   UNA 14-APR-2025
DEFINITION  Localization: Nucleus/Histones, Excitation: 587, Emission: 610.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1154)
  TITLE     Davidson lab - mCherry constructs
REFERENCE   2  (bases 1 to 1154)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 14, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: mCherry-H4-23
FEATURES             Location/Qualifiers
     source          1..1154
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     misc_feature    338..390
                     /label=MCS
                     /note="multiple cloning site"
     regulatory      398..407
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             404..1114
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(549..567)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     817..836
                     /label=mCherry-F
                     /note="mCherry, forward primer"
ORIGIN
        1 gacgtcagat ccgctagccg ccaccatgtc tggccgcggc aaaggcggga agggtcttgg
       61 caaaggcggc gctaagcgcc accgtaaagt actgcgcgac aatatccagg gcatcaccaa
      121 gccggccatc cggcgccttg ctcgccgcgg cggcgtgaag cgcatctccg gcctcatcta
      181 cgaggagact cgcggggtgc tgaaggtgtt cctggagaac gtgatccggg acgccgtgac
      241 ctatacagag cacgccaagc gcaagacggt caccgccatg gatgtggtct acgcgctcaa
      301 gcgccagggc cgcaccctct acggtttcgg tggggatctc gagctcaagc ttcgaattct
      361 gcagtcgacg gtaccgcggg cccgggatcc accggtcgcc accatggtga gcaagggcga
      421 ggaggataac atggccatca tcaaggagtt catgcgcttc aaggtgcaca tggagggctc
      481 cgtgaacggc cacgagttcg agatcgaggg cgagggcgag ggccgcccct acgagggcac
      541 ccagaccgcc aagctgaagg tgaccaaggg tggccccctg cccttcgcct gggacatcct
      601 gtcccctcag ttcatgtacg gctccaaggc ctacgtgaag caccccgccg acatccccga
      661 ctacttgaag ctgtccttcc ccgagggctt caagtgggag cgcgtgatga acttcgagga
      721 cggcggcgtg gtgaccgtga cccaggactc ctccctgcag gacggcgagt tcatctacaa
      781 ggtgaagctg cgcggcacca acttcccctc cgacggcccc gtaatgcaga agaagaccat
      841 gggctgggag gcctcctccg agcggatgta ccccgaggac ggcgccctga agggcgagat
      901 caagcagagg ctgaagctga aggacggcgg ccactacgac gctgaggtca agaccaccta
      961 caaggccaag aagcccgtgc agctgcccgg cgcctacaac gtcaacatca agttggacat
     1021 cacctcccac aacgaggact acaccatcgt ggaacagtac gaacgcgccg agggccgcca
     1081 ctccaccggc ggcatggacg agctgtacaa gtaaagcggc cgcgactcta gatcataatc
     1141 agccatacca catt
//
