LOCUS       sequence-85497-e        1120 bp    DNA     linear   UNA 14-APR-2025
DEFINITION  Localization: Endoplasmic Reticulum, Excitation: 587, Emission: 610.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1120)
  TITLE     Davidson lab - mCherry constructs
REFERENCE   2  (bases 1 to 1120)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 14, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: mCherry-Sec61-N-18
FEATURES             Location/Qualifiers
     source          1..1120
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             29..316
                     /codon_start=1
                     /gene="SEC61B"
                     /product="human Sec61-beta subunit of the translocon in the
                     endoplasmic reticulum"
                     /label=Sec61-beta
                     /translation="MPGPTPSGTNVGSSGRSPSKAVAARAAGSTVRQRKNASCGTRSAG
                     RTTSAGTGGMWRFYTEDSPGLKVGPVPVLVMSLLFIASVFMLHIWGKYTRS"
     regulatory      365..374
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             371..1081
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(516..534)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     784..803
                     /label=mCherry-F
                     /note="mCherry, forward primer"
ORIGIN
        1 tagtgacgtc agatccgcta gcgccaccat gcctggtccg acccccagtg gcactaacgt
       61 gggatcctca gggcgctctc ccagcaaagc agtggccgcc cgggcggcgg gatccactgt
      121 ccggcagagg aaaaatgcca gctgtgggac aaggagtgca ggccgcacaa cctcggcagg
      181 caccgggggg atgtggcgat tctacacaga agattcacct gggctcaaag ttggccctgt
      241 tccagtattg gttatgagtc ttctgttcat cgcttctgta tttatgttgc acatttgggg
      301 caagtacact cgttcgggac gaattctgca gtcgacggta ccgcgggccc gggatccacc
      361 ggtcgccacc atggtgagca agggcgagga ggataacatg gccatcatca aggagttcat
      421 gcgcttcaag gtgcacatgg agggctccgt gaacggccac gagttcgaga tcgagggcga
      481 gggcgagggc cgcccctacg agggcaccca gaccgccaag ctgaaggtga ccaagggtgg
      541 ccccctgccc ttcgcctggg acatcctgtc ccctcagttc atgtacggct ccaaggccta
      601 cgtgaagcac cccgccgaca tccccgacta cttgaagctg tccttccccg agggcttcaa
      661 gtgggagcgc gtgatgaact tcgaggacgg cggcgtggtg accgtgaccc aggactcctc
      721 cctgcaggac ggcgagttca tctacaaggt gaagctgcgc ggcaccaact tcccctccga
      781 cggccccgta atgcagaaga agaccatggg ctgggaggcc tcctccgagc ggatgtaccc
      841 cgaggacggc gccctgaagg gcgagatcaa gcagaggctg aagctgaagg acggcggcca
      901 ctacgacgct gaggtcaaga ccacctacaa ggccaagaag cccgtgcagc tgcccggcgc
      961 ctacaacgtc aacatcaagt tggacatcac ctcccacaac gaggactaca ccatcgtgga
     1021 acagtacgaa cgcgccgagg gccgccactc caccggcggc atggacgagc tgtacaagta
     1081 aagcggccgc gactctagat cataatcagc cataccacat
//
