LOCUS       sequence-85545-e        2130 bp    DNA     circular SYN 14-APR-2025
DEFINITION  HA-tag.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2130)
  AUTHORS   Radeck J, Kraft K, Bartels J, Cikovic T, Durr F, Emenegger J, 
            Kelterborn S, Sauer C, Fritz G, Gebhard S, Mascher T
  TITLE     The Bacillus BioBrick Box: generation and evaluation of essential 
            genetic building blocks for standardized work with Bacillus 
            subtilis.
  JOURNAL   J Biol Eng. 2013 Dec 2;7(1):29. doi: 10.1186/1754-1611-7-29.
  PUBMED    24295448
REFERENCE   2  (bases 1 to 2130)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 14, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Biol 
            Eng."; date: "2013-12-2"; volume: "7(1):29. doi"; pages: " 
            10.1186/1754-1611-7-29"
COMMENT     Sequence Label: HA-tag
FEATURES             Location/Qualifiers
     source          1..2130
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             46..72
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     misc_feature    82..102
                     /label=BioBrick suffix
                     /note="universal suffix for all parts"
     terminator      103..160
                     /label=his operon terminator
                     /note="This putative transcriptin terminator from the E. 
                     coli his operon has a 2-bp deletion introduced during 
                     synthesis. Its efficiency has not been determined."
     primer_bind     complement(184..201)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(355..943)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(435..454)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     terminator      complement(1126..1220)
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     CDS             complement(1241..1900)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     1815..1834
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     primer_bind     2062..2080
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     terminator      complement(2084..2127)
                     /label=bacterial terminator
                     /note="putative bacterial transcription terminator"
ORIGIN
        1 aattccgcgg ccgcttctag ataaggagga actactatgg ccggctatcc gtatgatgtt
       61 ccggattatg caaccggtta atactagtag cggccgctgc agtccggcaa aaaagggcaa
      121 ggtgtcacca ccctgccctt tttctttaaa accgaaaaga ttacttcgcg ttatgcaggc
      181 ttcctcgctc actgactcgc tgcgctcggt cgttcggctg cggcgagcgg tatcagctca
      241 ctcaaaggcg gtaatacggt tatccacaga atcaggggat aacgcaggaa agaacatgtg
      301 agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca
      361 caggctccgc ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa
      421 cccgacagga ctataaagat accaggcgtt tccccctgga agctccctcg tgcgctctcc
      481 tgttccgacc ctgccgctta ccggatacct gtccgccttt ctcccttcgg gaagcgtggc
      541 gctttctcat agctcacgct gtaggtatct cagttcggtg taggtcgttc gctccaagct
      601 gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc gccttatccg gtaactatcg
      661 tcttgagtcc aacccggtaa gacacgactt atcgccactg gcagcagcca ctggtaacag
      721 gattagcaga gcgaggtatg taggcggtgc tacagagttc ttgaagtggt ggcctaacta
      781 cggctacact agaagaacag tatttggtat ctgcgctctg ctgaagccag ttaccttcgg
      841 aaaaagagtt ggtagctctt gatccggcaa acaaaccacc gctggtagcg gtggtttttt
      901 tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct caagaagatc ctttgatctt
      961 ttctacgggg tctgacgctc agtggaacga aaactcacgt taagggattt tggtcatgag
     1021 attatcaaaa aggatcttca cctagatcct tttaaattaa aaatgaagtt ttaaatcaat
     1081 ctaaagtata tatgagtaaa cttggtctga cagctcgagg cttggattct caccaataaa
     1141 aaacgcccgg cggcaaccga gcgttctgaa caaatccaga tggagttctg aggtcattac
     1201 tggatctatc aacaggagtc caagcgagct cgatatcaaa ttacgccccg ccctgccact
     1261 catcgcagta ctgttgtaat tcattaagca ttctgccgac atggaagcca tcacaaacgg
     1321 catgatgaac ctgaatcgcc agcggcatca gcaccttgtc gccttgcgta taatatttgc
     1381 ccatggtgaa aacgggggcg aagaagttgt ccatattggc cacgtttaaa tcaaaactgg
     1441 tgaaactcac ccagggattg gctgagacga aaaacatatt ctcaataaac cctttaggga
     1501 aataggccag gttttcaccg taacacgcca catcttgcga atatatgtgt agaaactgcc
     1561 ggaaatcgtc gtggtattca ctccagagcg atgaaaacgt ttcagtttgc tcatggaaaa
     1621 cggtgtaaca agggtgaaca ctatcccata tcaccagctc accgtctttc attgccatac
     1681 gaaattccgg atgagcattc atcaggcggg caagaatgtg aataaaggcc ggataaaact
     1741 tgtgcttatt tttctttacg gtctttaaaa aggccgtaat atccagctga acggtctggt
     1801 tataggtaca ttgagcaact gactgaaatg cctcaaaatg ttctttacga tgccattggg
     1861 atatatcaac ggtggtatat ccagtgattt ttttctccat tttagcttcc ttagctcctg
     1921 aaaatctcga taactcaaaa aatacgcccg gtagtgatct tatttcatta tggtgaaagt
     1981 tggaacctct tacgtgcccg atcaactcga gtgccacctg acgtctaaga aaccattatt
     2041 atcatgacat taacctataa aaataggcgt atcacgaggc agaatttcag ataaaaaaaa
     2101 tccttagctt tcgctaagga tgatttctgg
//
