LOCUS       sequence-85695-e        1588 bp    DNA     linear   UNA 14-APR-2025
DEFINITION  Localization: Membrane, Excitation: 399, Emission: 456.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1588)
  AUTHORS   Subach OM, Cranfill PJ, Davidson MW, Verkhusha VV
  TITLE     An enhanced monomeric blue fluorescent protein with the high 
            chemical stability of the chromophore.
  JOURNAL   PLoS One. 2011;6(12):e28674. Epub 2011 Dec 8.
  PUBMED    22174863
REFERENCE   2  (bases 1 to 1588)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 14, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2011"; volume: "6(12)"; pages: "e28674. Epub 2011 Dec 8"
COMMENT     Sequence Label: mTagBFP2-Dectin1A-C-10
FEATURES             Location/Qualifiers
     source          1..1588
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      22..31
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             28..738
                     /codon_start=1
                     /product="enhanced monomeric blue fluorescent protein 
                     (Subach et al., 2011)"
                     /label=mTagBFP2
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELIKENMHMKLYMEGTVDNHHFKCTSEGEGKPYEGTQTM
                     RIKVVEGGPLPFAFDILATSFLYGSKTFINHTQGIPDFFKQSFPEGFTWERVTTYEDGG
                     VLTATQDTSLQDGCLIYNVKIRGVNFTSNGPVMQKKTLGWEAFTETLYPADGGLEGRND
                     MALKLVGGSHLIANAKTTYRSKKPAKNLKMPGVYYVDYRLERIKEANNETYVEQHEVAV
                     ARYCDLPSKLGHKLN"
ORIGIN
        1 acgtcagatc cgctagcggt ggccaccatg gtgtctaagg gcgaagagct gattaaggag
       61 aacatgcaca tgaagctgta catggagggc accgtggaca accatcactt caagtgcaca
      121 tccgagggcg aaggcaagcc ctacgagggc acccagacca tgagaatcaa ggtggtcgag
      181 ggcggccctc tccccttcgc cttcgacatc ctggctacta gcttcctcta cggcagcaag
      241 accttcatca accacaccca gggcatcccc gacttcttca agcagtcctt ccctgagggc
      301 ttcacatggg agagagtcac cacatacgaa gacgggggcg tgctgaccgc tacccaggac
      361 accagcctcc aggacggctg cctcatctac aacgtcaaga tcagaggggt gaacttcaca
      421 tccaacggcc ctgtgatgca gaagaaaaca ctcggctggg aggccttcac cgagacgctg
      481 taccccgctg acggcggcct ggaaggcaga aacgacatgg ccctgaagct cgtgggcggg
      541 agccatctga tcgcaaacgc caagaccaca tatagatcca agaaacccgc taagaacctc
      601 aagatgcctg gcgtctacta tgtggactac agactggaaa gaatcaagga ggccaacaac
      661 gagacctacg tcgagcagca cgaggtggca gtggccagat actgcgacct ccctagcaaa
      721 ctggggcaca agcttaattc cggactcaga tctggcagca gtggaggtga atatcatcct
      781 gatttagaaa atttggatga agatggatat actcaattac acttcgactc tcaaagcaat
      841 accaggatag ctgttgtttc agagaaagga tcgtgtgctg catctcctcc ttggcgcctc
      901 attgctgtaa ttttgggaat cctatgcttg gtaatactgg tgatagctgt ggtcctgggt
      961 accatggcta tttggagatc caattcagga agcaacacat tggagaatgg ctactttcta
     1021 tcaagaaata aagagaacca cagtcaaccc acacaatcat ctttagaaga cagtgtgact
     1081 cctaccaaag ctgtcaaaac cacaggggtt ctttccagcc cttgtcctcc taattggatt
     1141 atatatgaga agagctgtta tctattcagc atgtcactaa attcctggga tggaagtaaa
     1201 agacaatgct ggcaactggg ctctaatctc ctaaagatag acagctcaaa tgaattggga
     1261 tttatagtaa aacaagtgtc ttcccaacct gataattcat tttggatagg cctttctcgg
     1321 ccccagactg aagtaccatg gctctgggag gatggatcaa cattctcttc taacttattt
     1381 cagatcagaa ccacagctac ccaagaaaac ccatctccaa attgtgtatg gattcacgtg
     1441 tcagtcattt atgaccaact gtgtagtgtg ccctcatata gtatttgtga gaagaagttt
     1501 tcaatgtaag aattctgcag tcgacggtac cgcgggcccg ggatccaccg gatctagata
     1561 actgatcata atcagccata ccacattt
//
