LOCUS       sequence-85931-e        2377 bp    DNA     linear   UNA 14-APR-2025
DEFINITION  Localization: Membrane Receptor, Excitation: 434, Emission: 474.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2377)
  TITLE     Davidson Cyan Fluorescent Proteins
REFERENCE   2  (bases 1 to 2377)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 14, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: mTurquoise-ALK5-N-13
FEATURES             Location/Qualifiers
     source          1..2377
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      49..58
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     regulatory      1597..1606
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(1603..1605,1606..1608,1609..2322)
                     /codon_start=1
                     /product="enhanced monomeric variant of CFP (Goedhart et 
                     al., 2010)"
                     /label=mTurquoise
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLSWGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYISDNVYITADKQKNGIK
                     ANFKIRHNIEDGGVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(1648..1669)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(1909..1928)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     2256..2277
                     /label=EGFP-C
                     /note="EGFP, forward primer"
ORIGIN
        1 gcccatgcta gtgacgtcag atccgctagc gctaccggac tcagatctgc caccatggag
       61 gcggcggtcg ctgctccgcg tccccggctg ctcctcctcg tgctggcggc ggcggcggcg
      121 gcggcggcgg cgctgctccc gggggcgacg gcgttacagt gtttctgcca cctctgtaca
      181 aaagacaatt ttacttgtgt gacagatggg ctctgctttg tctctgtcac agagaccaca
      241 gacaaagtta tacacaacag catgtgtata gctgaaattg acttaattcc tcgagatagg
      301 ccgtttgtat gtgcaccctc ttcaaaaact gggtctgtga ctacaacata ttgctgcaat
      361 caggaccatt gcaataaaat agaacttcca actactgtaa agtcatcacc tggccttggt
      421 cctgtggaac tggcagctgt cattgctgga ccagtgtgct tcgtctgcat ctcactcatg
      481 ttgatggtct atatctgcca caaccgcact gtcattcacc atcgagtgcc aaatgaagag
      541 gacccttcat tagatcgccc ttttatttca gagggtacta cgttgaaaga cttaatttat
      601 gatatgacaa cgtcaggttc tggctcaggt ttaccattgc ttgttcagag aacaattgcg
      661 agaactattg tgttacaaga aagcattggc aaaggtcgat ttggagaagt ttggagagga
      721 aagtggcggg gagaagaagt tgctgttaag atattctcct ctagagaaga acgttcgtgg
      781 ttccgtgagg cagagattta tcaaactgta atgttacgtc atgaaaacat cctgggattt
      841 atagcagcag acaataaaga caatggtact tggactcagc tctggttggt gtcagattat
      901 catgagcatg gatccctttt tgattactta aacagataca cagttactgt ggaaggaatg
      961 ataaaacttg ctctgtccac ggcgagcggt cttgcccatc ttcacatgga gattgttggt
     1021 acccaaggaa agccagccat tgctcataga gatttgaaat caaagaatat cttggtaaag
     1081 aagaatggaa cttgctgtat tgcagactta ggactggcag taagacatga ttcagccaca
     1141 gataccattg atattgctcc aaaccacaga gtgggaacaa aaaggtacat ggcccctgaa
     1201 gttctcgatg attccataaa tatgaaacat tttgaatcct tcaaacgtgc tgacatctat
     1261 gcaatgggct tagtattctg ggaaattgct cgacgatgtt ccattggtgg aattcatgaa
     1321 gattaccaac tgccttatta tgatcttgta ccttctgacc catcagttga agaaatgaga
     1381 aaagttgttt gtgaacagaa gttaaggcca aatatcccaa acagatggca gagctgtgaa
     1441 gccttgagag taatggctaa aattatgaga gaatgttggt atgccaatgg agcagctagg
     1501 cttacagcat tgcggattaa gaaaacatta tcgcaactca gtcaacagga aggcatcaaa
     1561 atgggttccg ggggtgcggg cggcggagga ccggtcgcca ccatggtgag caagggcgag
     1621 gagctgttca ccggggtggt gcccatcctg gtcgagctgg acggcgacgt aaacggccac
     1681 aagttcagcg tgtccggcga gggcgagggc gatgccacct acggcaagct gaccctgaag
     1741 ttcatctgca ccaccggcaa gctgcccgtg ccctggccca ccctcgtgac caccctgtcc
     1801 tggggcgtgc agtgcttcgc ccgctacccc gaccacatga agcagcacga cttcttcaag
     1861 tccgccatgc ccgaaggcta cgtccaggag cgcaccatct tcttcaagga cgacggcaac
     1921 tacaagaccc gcgccgaggt gaagttcgag ggcgacaccc tggtgaaccg catcgagctg
     1981 aagggcatcg acttcaagga ggacggcaac atcctggggc acaagctgga gtacaactac
     2041 atcagcgaca acgtctatat caccgccgac aagcagaaga acggcatcaa ggccaacttc
     2101 aagatccgcc acaacatcga ggacggcggc gtgcagctcg ccgaccacta ccagcagaac
     2161 acccccatcg gcgacggccc cgtgctgctg cccgacaacc actacctgag cacccagtcc
     2221 aagctgagca aagaccccaa cgagaagcgc gatcacatgg tcctgctgga gttcgtgacc
     2281 gccgccggga tcactctcgg catggacgag ctgtacaagt aaagcggccg cgactctaga
     2341 tcataatcag ccataccaca ttgtagaaag taccccc
//
