LOCUS       sequence-85939-e        2401 bp    DNA     linear   UNA 15-APR-2025
DEFINITION  Localization: Membrane Receptor, Excitation: 434, Emission: 474.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2401)
  TITLE     Davidson Cyan Fluorescent Proteins
REFERENCE   2  (bases 1 to 2401)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: mTurquoise-ATG13-C-18
FEATURES             Location/Qualifiers
     source          1..2401
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      28..37
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(34..36,37..39,40..750)
                     /codon_start=1
                     /product="enhanced monomeric variant of CFP (Goedhart et 
                     al., 2010)"
                     /label=mTurquoise
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLSWGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYISDNVYITADKQKNGIK
                     ANFKIRHNIEDGGVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(79..100)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(340..359)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     687..708
                     /label=EGFP-C
                     /note="EGFP, forward primer"
ORIGIN
        1 acgtcagatc cgctagcgct accggtcgcc accatggtga gcaagggcga ggagctgttc
       61 accggggtgg tgcccatcct ggtcgagctg gacggcgacg taaacggcca caagttcagc
      121 gtgtccggcg agggcgaggg cgatgccacc tacggcaagc tgaccctgaa gttcatctgc
      181 accaccggca agctgcccgt gccctggccc accctcgtga ccaccctgtc ctggggcgtg
      241 cagtgcttcg cccgctaccc cgaccacatg aagcagcacg acttcttcaa gtccgccatg
      301 cccgaaggct acgtccagga gcgcaccatc ttcttcaagg acgacggcaa ctacaagacc
      361 cgcgccgagg tgaagttcga gggcgacacc ctggtgaacc gcatcgagct gaagggcatc
      421 gacttcaagg aggacggcaa catcctgggg cacaagctgg agtacaacta catcagcgac
      481 aacgtctata tcaccgccga caagcagaag aacggcatca aggccaactt caagatccgc
      541 cacaacatcg aggacggcgg cgtgcagctc gccgaccact accagcagaa cacccccatc
      601 ggcgacggcc ccgtgctgct gcccgacaac cactacctga gcacccagtc caagctgagc
      661 aaagacccca acgagaagcg cgatcacatg gtcctgctgg agttcgtgac cgccgccggg
      721 atcactctcg gcatggacga gctgtacaag tccggactca gatctagtag cggaggtagc
      781 gcatccggcg gaagcggagg aagcgaaact gatctcaatt cccaggacag aaaggacctg
      841 gacaagttta ttaaattttt tgccctcaag actgtccaag tgattgtcca ggctcggctt
      901 ggtgaaaaga tttgcactcg ttcatcatct tctccaacgg gttcagattg gttcaactta
      961 gcaatcaaag acatcccaga ggttacacat gaagcaaaga aggcactggc aggacagctg
     1021 cctgcagtcg ggaggtccat gtgtgtggag atttcactta agacttctga gggagattcc
     1081 atggagctgg aaatatggtg tcttgaaatg aatgaaaagt gtgataaaga aatcaaagtt
     1141 tcctacacgg tgtacaacag actgtcattg ctgctgaagt cccttcttgc tataactagg
     1201 gtgacaccag cctataggct ctccaggaaa caagggcatg aatatgtcat attatacagg
     1261 atatattttg gagaagttca gctgagtggc ttaggagaag gcttccagac agttcgtgtt
     1321 gggacagtgg gcacccctgt gggcaccatc actctttctt gtgcttacag aattaacttg
     1381 gcattcatgt ctaccaggca atttgagagg accccaccta tcatggggat tattattgat
     1441 cactttgtgg accgtcccta tcccagctcc tctcccatgc acccctgcaa ttacagaact
     1501 gctggtgagg acactggagt aatatacccg tctgtagaag actctcaaga agtgtgtacc
     1561 acctcttttt ccacctcccc accatcccag ctctcaagct ctcgcctttc ctatcagcct
     1621 gctgccctgg gcgttggatc agctgacctg gcttatccag tagtgtttgc tgctggctta
     1681 aatgctacac accctcacca gctgatggtt cctgggaagg aaggtggggt accccttgct
     1741 cccaaccagc ctgtccatgg tacccaggct gaccaggaga gactggcaac ctgcacccct
     1801 tctgacagaa cccactgtgc tgccacaccc tccagtagtg aggatactga aaccgtatca
     1861 aacagcagtg agggacgggc ctcccctcac gatgtcttgg agaccatctt tgtccgaaaa
     1921 gtgggggctt ttgtcaacaa acccattaac caggtgaccc tgacgagttt ggatataccc
     1981 tttgccatgt ttgctcccaa gaatttggag ctggaggata ccgatccaat ggtgaatcct
     2041 ccagattccc cagagactga atctcctctc cagggcagcc tgcactcaga tggctccagc
     2101 gggggcagca gtggcaatac ccatgatgac tttgttatga tagactttaa accagctttt
     2161 tctaaagatg acattcttcc gatggacctg gggaccttct atcgggagtt tcagaaccca
     2221 cctcagctga gcagcctctc catagatatt ggagcacagt ccatggctga agacttggac
     2281 tcattaccag agaagctggc tgtgcatgag aagaatgtcc gcgagtttga tgcctttgtg
     2341 gaaaccctgc agtaaggatc caccggatct agataactga tcataatcag ccataccaca
     2401 c
//
