LOCUS       sequence-85964-e        1225 bp    DNA     linear   UNA 14-APR-2025
DEFINITION  Localization: Nucleus/Histones, Excitation: 434, Emission: 474.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1225)
  TITLE     Davidson Cyan Fluorescent Proteins
REFERENCE   2  (bases 1 to 1225)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 14, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: mTurquoise-H2B-6
FEATURES             Location/Qualifiers
     source          1..1225
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             65..442
                     /codon_start=1
                     /gene="HIST1H2BJ"
                     /product="human histone H2B"
                     /label=H2B
                     /translation="MPEPAKSAPAPKKGSKKAVTKAQKKGGKKRKRSRKESYSIYVYKV
                     LKQVHPDTGISSKAMGIMNSFVNDIFERIAGEASRLAHYNKRSTITSREIQTAVRLLLP
                     GELAKHAVSEGTKAITKYTSAK"
     regulatory      455..464
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(461..463,464..466,467..1180)
                     /codon_start=1
                     /product="enhanced monomeric variant of CFP (Goedhart et 
                     al., 2010)"
                     /label=mTurquoise
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLSWGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYISDNVYITADKQKNGIK
                     ANFKIRHNIEDGGVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(506..527)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(767..786)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     1114..1135
                     /label=EGFP-C
                     /note="EGFP, forward primer"
ORIGIN
        1 tagcgctacc ggactcagat ctcgagctca agcttcgaat tctgcagtcg acggtaccgc
       61 caccatgcca gagccagcga agtctgctcc cgccccgaaa aagggctcca agaaggcggt
      121 gactaaggcg cagaagaaag gcggcaagaa gcgcaagcgc agccgcaagg agagctattc
      181 catctatgtg tacaaggttc tgaagcaggt ccaccctgac accggcattt cgtccaaggc
      241 catgggcatc atgaattcgt ttgtgaacga cattttcgag cgcatcgcag gtgaggcttc
      301 ccgcctggcg cattacaaca agcgctcgac catcacctcc agggagatcc agacggccgt
      361 gcgcctgctg ctgcctgggg agttggccaa gcacgccgtg tccgagggta ctaaggccat
      421 caccaagtac accagcgcta aggatccacc ggtcgccacc atggtgagca agggcgagga
      481 gctgttcacc ggggtggtgc ccatcctggt cgagctggac ggcgacgtaa acggccacaa
      541 gttcagcgtg tccggcgagg gcgagggcga tgccacctac ggcaagctga ccctgaagtt
      601 catctgcacc accggcaagc tgcccgtgcc ctggcccacc ctcgtgacca ccctgtcctg
      661 gggcgtgcag tgcttcgccc gctaccccga ccacatgaag cagcacgact tcttcaagtc
      721 cgccatgccc gaaggctacg tccaggagcg caccatcttc ttcaaggacg acggcaacta
      781 caagacccgc gccgaggtga agttcgaggg cgacaccctg gtgaaccgca tcgagctgaa
      841 gggcatcgac ttcaaggagg acggcaacat cctggggcac aagctggagt acaactacat
      901 cagcgacaac gtctatatca ccgccgacaa gcagaagaac ggcatcaagg ccaacttcaa
      961 gatccgccac aacatcgagg acggcggcgt gcagctcgcc gaccactacc agcagaacac
     1021 ccccatcggc gacggccccg tgctgctgcc cgacaaccac tacctgagca cccagtccaa
     1081 gctgagcaaa gaccccaacg agaagcgcga tcacatggtc ctgctggagt tcgtgaccgc
     1141 cgccgggatc actctcggca tggacgagct gtacaagtaa agcggccgcg actctagatc
     1201 ataatcagcc ataccacatt gagaa
//
