LOCUS       sequence-85977-e        1886 bp    DNA     linear   UNA 15-APR-2025
DEFINITION  Localization: Microtubules, Excitation: 434, Emission: 474.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1886)
  TITLE     Davidson Cyan Fluorescent Proteins
REFERENCE   2  (bases 1 to 1886)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: mTurquoise-MAPTau-N-10
FEATURES             Location/Qualifiers
     source          1..1886
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      35..44
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     regulatory      1121..1130
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(1127..1129,1130..1132,1133..1846)
                     /codon_start=1
                     /product="enhanced monomeric variant of CFP (Goedhart et 
                     al., 2010)"
                     /label=mTurquoise
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLSWGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYISDNVYITADKQKNGIK
                     ANFKIRHNIEDGGVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(1172..1193)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(1433..1452)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     1780..1801
                     /label=EGFP-C
                     /note="EGFP, forward primer"
ORIGIN
        1 agatccgcta gcgctaccgg actcagatct cgaggccacc atggctgagc cccgccagga
       61 gttcgaagtg atggaagatc acgctgggac gtacgggttg ggggacagga aagatcaggg
      121 gggctacacc atgcaccaag accaagaggg tgacacggac gctggcctga aagctgaaga
      181 agcaggcatt ggagacaccc ccagcctgga agacgaagct gctggtcacg tgacccaagc
      241 tcgcatggtc agtaaaagca aagacgggac tggaagcgat gacaaaaaag ccaagggggc
      301 tgatggtaaa acgaagatcg ccacaccgcg gggagcagcc cctccaggcc agaagggcca
      361 ggccaacgcc accaggattc cagcaaaaac cccgcccgct ccaaagacac cacccagctc
      421 tggtgaacct ccaaaatcag gggatcgcag cggctacagc agccccggct ccccaggcac
      481 tcccggcagc cgctcccgca ccccgtccct tccaacccca cccacccggg agcccaagaa
      541 ggtggcagtg gtccgtactc cacccaagtc gccgtcttcc gccaagagcc gcctgcagac
      601 agcccccgtg cccatgccag acctgaagaa tgtcaagtcc aagatcggct ccactgagaa
      661 cctgaagcac cagccgggag gcgggaaggt gcaaatagtc tacaaaccag ttgacctgag
      721 caaggtgacc tccaagtgtg gctcattagg caacatccat cataaaccag gaggtggcca
      781 ggtggaagta aaatctgaga agcttgactt caaggacaga gtccagtcga agattgggtc
      841 cctggacaat atcacccacg tccctggcgg aggaaataaa aagattgaaa cccacaagct
      901 gaccttccgc gagaacgcca aagccaagac agaccacggg gcggagatcg tgtacaagtc
      961 gccagtggtg tctggggaca cgtctccacg gcatctcagc aatgtctcct ccaccggcag
     1021 catcgacatg gtagactcgc cccagctcgc cacgctagct gacgaggtgt ctgcctccct
     1081 ggccaagcag ggtttgagct ccggagggga tccaccggtc gccaccatgg tgagcaaggg
     1141 cgaggagctg ttcaccgggg tggtgcccat cctggtcgag ctggacggcg acgtaaacgg
     1201 ccacaagttc agcgtgtccg gcgagggcga gggcgatgcc acctacggca agctgaccct
     1261 gaagttcatc tgcaccaccg gcaagctgcc cgtgccctgg cccaccctcg tgaccaccct
     1321 gtcctggggc gtgcagtgct tcgcccgcta ccccgaccac atgaagcagc acgacttctt
     1381 caagtccgcc atgcccgaag gctacgtcca ggagcgcacc atcttcttca aggacgacgg
     1441 caactacaag acccgcgccg aggtgaagtt cgagggcgac accctggtga accgcatcga
     1501 gctgaagggc atcgacttca aggaggacgg caacatcctg gggcacaagc tggagtacaa
     1561 ctacatcagc gacaacgtct atatcaccgc cgacaagcag aagaacggca tcaaggccaa
     1621 cttcaagatc cgccacaaca tcgaggacgg cggcgtgcag ctcgccgacc actaccagca
     1681 gaacaccccc atcggcgacg gccccgtgct gctgcccgac aaccactacc tgagcaccca
     1741 gtccaagctg agcaaagacc ccaacgagaa gcgcgatcac atggtcctgc tggagttcgt
     1801 gaccgccgcc gggatcactc tcggcatgga cgagctgtac aagtaaagcg gccgcgactc
     1861 tagatcataa tcagccatac cacatt
//
