LOCUS       sequence-85982-e        2611 bp    DNA     linear   UNA 15-APR-2025
DEFINITION  Localization: Centrosomes, Excitation: 434, Emission: 474.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2611)
  TITLE     Davidson Cyan Fluorescent Proteins
REFERENCE   2  (bases 1 to 2611)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: mTurquoise-PLK1-N-16
FEATURES             Location/Qualifiers
     source          1..2611
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      1863..1872
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(1869..1871,1872..1874,1875..2588)
                     /codon_start=1
                     /product="enhanced monomeric variant of CFP (Goedhart et 
                     al., 2010)"
                     /label=mTurquoise
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLSWGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYISDNVYITADKQKNGIK
                     ANFKIRHNIEDGGVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(1914..1935)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(2175..2194)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     2522..2543
                     /label=EGFP-C
                     /note="EGFP, forward primer"
ORIGIN
        1 ctagcgccac catgagtgct gcagtgactg cagggaagct ggcacgggca ccggccgacc
       61 ctgggaaagc cggggtcccc ggagttgcag ctcccggagc tccggcggcg gctccaccgg
      121 cgaaagagat cccggaggtc ctagtggacc cacgcagccg gcggcgctat gtgcggggcc
      181 gctttttggg caagggcggc tttgccaagt gcttcgagat ctcggacgcg gacaccaagg
      241 aggtgttcgc gggcaagatt gtgcctaagt ctctgctgct caagccgcac cagagggaga
      301 agatgtccat ggaaatatcc attcaccgca gcctcgccca ccagcacgtc gtaggattcc
      361 acggcttttt cgaggacaac gacttcgtgt tcgtggtgtt ggagctctgc cgccggaggt
      421 ctctcctgga gctgcacaag aggaggaaag ccctgactga gcctgaggcc cgatactacc
      481 tacggcaaat tgtgcttggc tgccagtacc tgcaccgaaa ccgagttatt catcgagacc
      541 tcaagctggg caaccttttc ctgaatgaag atctggaggt gaaaataggg gattttggac
      601 tggcaaccaa agtcgaatat gacggggaga ggaagaagac cctgtgtggg actcctaatt
      661 acatagctcc cgaggtgctg agcaagaaag ggcacagttt cgaggtggat gtgtggtcca
      721 ttgggtgtat catgtatacc ttgttagtgg gcaaaccacc ttttgagact tcttgcctaa
      781 aagagaccta cctccggatc aagaagaatg aatacagtat tcccaagcac atcaaccccg
      841 tggccgcctc cctcatccag aagatgcttc agacagatcc cactgcccgc ccaaccatta
      901 acgagctgct taatgacgag ttctttactt ctggctatat ccctgcccgt ctccccatca
      961 cctgcctgac cattccacca aggttttcga ttgctcccag cagcctggac cccagcaacc
     1021 ggaagcccct cacagtcctc aataaaggct tggagaaccc cctgcctgag cgtccccggg
     1081 aaaaagaaga accagtggtt cgagagacag gtgaggtggt cgactgccac ctcagtgaca
     1141 tgctgcagca gctgcacagt gtcaatgcct ccaagccctc ggagcgtggg ctggtcaggc
     1201 aagaggaggc tgaggatcct gcctgcatcc ccatcttctg ggtcagcaag tgggtggact
     1261 attcggacaa gtacggcctt gggtatcagc tctgtgataa cagcgtgggg gtgctcttca
     1321 atgactcaac acgcctcatc ctctacaatg atggtgacag cctgcagtac atagagcgtg
     1381 acggcactga gtcctacctc accgtgagtt cccatcccaa ctccttgatg aagaagatca
     1441 ccctccttaa atatttccgc aattacatga gcgagcactt gctgaaggca ggtgccaaca
     1501 tcacgccgcg cgaaggtgat gagctcgccc ggctgcccta cctacggacc tggttccgca
     1561 cccgcagcgc catcatcctg cacctcagca acggcagcgt gcagatcaac ttcttccagg
     1621 atcacaccaa gctcatcttg tgcccactga tggcagccgt gacctacatc gacgagaagc
     1681 gggacttccg cacataccgc ctgagtctcc tggaggagta cggctgctgc aaggagctgg
     1741 ccagccggct ccgctacgcc cgcactatgg tggacaagct gctgagctca cgctcggcca
     1801 gcaaccgtct caaggcctcc ggtagcggca gcgcatccgg cggaagcgga agcggaccgg
     1861 tcgccaccat ggtgagcaag ggcgaggagc tgttcaccgg ggtggtgccc atcctggtcg
     1921 agctggacgg cgacgtaaac ggccacaagt tcagcgtgtc cggcgagggc gagggcgatg
     1981 ccacctacgg caagctgacc ctgaagttca tctgcaccac cggcaagctg cccgtgccct
     2041 ggcccaccct cgtgaccacc ctgtcctggg gcgtgcagtg cttcgcccgc taccccgacc
     2101 acatgaagca gcacgacttc ttcaagtccg ccatgcccga aggctacgtc caggagcgca
     2161 ccatcttctt caaggacgac ggcaactaca agacccgcgc cgaggtgaag ttcgagggcg
     2221 acaccctggt gaaccgcatc gagctgaagg gcatcgactt caaggaggac ggcaacatcc
     2281 tggggcacaa gctggagtac aactacatca gcgacaacgt ctatatcacc gccgacaagc
     2341 agaagaacgg catcaaggcc aacttcaaga tccgccacaa catcgaggac ggcggcgtgc
     2401 agctcgccga ccactaccag cagaacaccc ccatcggcga cggccccgtg ctgctgcccg
     2461 acaaccacta cctgagcacc cagtccaagc tgagcaaaga ccccaacgag aagcgcgatc
     2521 acatggtcct gctggagttc gtgaccgccg ccgggatcac tctcggcatg gacgagctgt
     2581 acaagtaaag cggccgcgac tctagatcat a
//
