LOCUS       sequence-86146-e         926 bp    DNA     linear   UNA 14-APR-2025
DEFINITION  Lentivirus containing mouse Hey2 coding region.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 926)
  AUTHORS   Hartman ME, Librande JR, Medvedev IO, Ahmad RN, Moussavi-Harami F, 
            Gupta PP, Chien WM, Chin MT
  TITLE     An optimized and simplified system of mouse embryonic stem cell 
            cardiac differentiation for the assessment of differentiation 
            modifiers.
  JOURNAL   PLoS One. 2014 Mar 25;9(3):e93033. doi: 
            10.1371/journal.pone.0093033. eCollection 2014.
  PUBMED    24667642
REFERENCE   2  (bases 1 to 926)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 14, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2014-03-25"; volume: "9(3):e93033. doi"; pages: " 
            10.1371/journal.pone.0093033. eCollection 2014"
COMMENT     Sequence Label: iDuet101a-mCHF1
FEATURES             Location/Qualifiers
     source          1..926
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(35..56)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     CDS             complement(join(191..703,704..706,707..709))
                     /codon_start=1
                     /product="N-terminal fragment of mVenus for use in 
                     bimolecular fluorescence complementation (BiFC) (Kodama and
                     Hu, 2010)"
                     /label=VN173
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIE"
     primer_bind     384..403
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     643..664
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     regulatory      706..715
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 gctncgtcat gccgagngtg atcccggcgg cggtcacgaa ctccagcagg accatgtgat
       61 cgcgcttctc gttggggtct ttgctcaggg cggactgggt gctcaggtag tggttgtcgg
      121 gcagcagcac ggggccgtcg ccgatggggg tgttctgctg gtagtggtcg gcgagctgca
      181 cgctgccgtc ctcgatgttg tggcggatct tgaagttcac cttgatgccg ttcttctgct
      241 tgtcggccat gatatagacg ttgtggctgt tgtagttgta ctccagcttg tgccccagga
      301 tgttgccgtc ctccttgaag tcgatgccct tcagctcgat gcggttcacc agggtgtcgc
      361 cctcgaactt cacctcggcg cgggtcttgt agttgccgtc gtccttgaag aagatggtgc
      421 gctcctggac gtagccttcg ggcatggcgg acttgaagaa gtcgtgctgc ttcatgtggt
      481 cggggtagcg gctgaagcac tgcacgccgt aggtcagggt ggtcacgagg gtgggccagg
      541 gcacgggcag cttgccggtg gtgcagatga acttcagggt cagcttgccg taggtggcat
      601 cgccctcgcc ctcgccggac acgctgaact tgtggccgtt tacgtcgccg tccagctcga
      661 ccaggatggg caccaccccg gtgaacagct cctcgccctt gctcaccatg gtggcgaccg
      721 gtacccgggg atcctctaga tgcatgctcg agcggccgcc agtgtgatgg atatctgcag
      781 aattcgccct tggtggcgac gactcctgga gcccgtcagt atcggcggaa ttccggagag
      841 attcaagaat aagttaaaag gctccaactt ctgtccccca gggttggtaa ggnntnnttg
      901 tttnnccnnn nngcttnncn gnnnaa
//
