LOCUS       sequence-86313-e         803 bp    DNA     linear   UNA 15-APR-2025
DEFINITION  Bacterial expression of human XRCC1-K176R C-terminally fused to GyrA
            intein and GST.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 803)
  AUTHORS   Weber AR, Schuermann D, Schar P
  TITLE     Versatile Recombinant SUMOylation System for the Production of 
            SUMO-Modified Protein.
  JOURNAL   PLoS One. 2014 Jul 9;9(7):e102157. doi: 
            10.1371/journal.pone.0102157. eCollection 2014.
  PUBMED    25007328
REFERENCE   2  (bases 1 to 803)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2014-07-9"; volume: "9(7):e102157. doi"; pages: " 
            10.1371/journal.pone.0102157. eCollection 2014"
COMMENT     Sequence Label: pTXG-hXRCC1-K176R
FEATURES             Location/Qualifiers
     source          1..803
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(42..635)
                     /codon_start=1
                     /gene="Mycobacterium xenopi gyrA"
                     /product="modified GyrA intein (Southworth et al., 1999)"
                     /label=Mxe GyrA intein
                     /note="N-terminal cleavage of this intein can be induced by
                     thiols."
                     /translation="CICGDALVALPEGESVRIADIVPGARPNSDNAIDLKVLDRHGNPV
                     LADRLFHSGEHPVYTVRTVEGLRVTGTANHPLLCLVDVAGVPTLLWKLIDEIKPGDYAV
                     IQRSAFSVDCAGFARGKPEFAPTTYTVGVPGLVRFLEAHHRDPDAQAIADELTDGRFYY
                     AKVASVTDAGVQPVYSLRVDTADHAFITNGFVSHA"
ORIGIN
        1 attccggatc cgaggcctga gttcagaccg gtgaggccag tagcgtggct gacgaacccg
       61 ttcgtgataa acgcgtggtc tgccgtgtcg acacgaaggc tatacaccgg ctgcacgccg
      121 gcgtcggtga cactggcgac tttcgcgtag tagaaccgcc cgtcggtcag ctcgtcggcg
      181 atagcttggg cgtccgggtc tcggtggtgt gcttccaaga aacgcaccag tccagggacg
      241 ccgactgtgt aggttgtggg cgcaaattcg ggtttcccgc gggcaaaacc tgcacagtcg
      301 acgctgaatg cgctgcgttg aatcaccgcg taatcgcccg gcttgatttc gtcgatcagc
      361 ttccacagca gggtcggcac cccggcgacg tcgaccaaac acaacaacgg gtggttcgcg
      421 gtgcccgtca cacgcagacc ttcgaccgta cgcaccgtgt acaccggatg ctcgccggag
      481 tggaacagcc ggtcggcgag cacgggattg ccatgccggt caaggacttt caggtcgatg
      541 gcgttgtcac tgttgggccg cgcacccggc acgatgtcgg cgatgcgtac cgactcgccc
      601 tcgggtaggg caactagtgc atctccgcag atgcacatct cgaggaattc ggcttgcggc
      661 accaccccat agagctggtg aggaagtaac ttctgcttct cattgcaact gtagatccat
      721 cggggacgaa cgaatgccag ggaggggttg tccatcaggg cctcctcaaa gctgggatcc
      781 cattcctgtg ctgtgatcac aaa
//
