LOCUS       sequence-86467-e         870 bp    DNA     linear   UNA 14-APR-2025
DEFINITION  E.coli expression of untagged mature human SUMO1 (amino acids 1-97).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 870)
  AUTHORS   Pichler A, Gast A, Seeler JS, Dejean A, Melchior F
  TITLE     The nucleoporin RanBP2 has SUMO1 E3 ligase activity.
  JOURNAL   Cell. 2002 Jan 11;108(1):109-20.
  PUBMED    11792325
REFERENCE   2  (bases 1 to 870)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 14, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell."; 
            date: "2002-01-11"; volume: "108(1)"; pages: "109-20"
COMMENT     Sequence Label: pET11a-SUMO1
FEATURES             Location/Qualifiers
     source          1..870
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     42..61
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        42..60
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    61..85
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     RBS             100..122
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     primer_bind     complement(520..538)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      534..581
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     promoter        complement(join(756..761,762..778,779..784))
                     /label=tet promoter
                     /note="E. coli promoter for tetracycline efflux protein 
                     gene"
     primer_bind     complement(811..829)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
ORIGIN
        1 tgcgtccggc gtagaggatc gagatctcga tcccgcgaaa ttaatacgac tcactatagg
       61 ggaattgtga gcggataaca attcccctct agaaataatt ttgtttaact ttaagaagga
      121 gatatacata tgtctgacca ggaggcaaaa ccttcaactg aggacttggg ggataagaag
      181 gaaggtgaat atattaaact caaagtcatt ggacaggata gcagtgagat tcacttcaaa
      241 gtgaaaatga caacacatct caagaaactc aaagaatcat actgtcaaag acagggtgtt
      301 ccaatgaatt cactcaggtt tctctttgag ggtcagagaa ttgctgataa tcatactcca
      361 aaagaactgg gaatggagga agaagatgtg attgaagttt atcaggaaca aacggggggt
      421 taggattcat ttaagccgga attccagcac actggcggcc gttactagtg gatccggctg
      481 ctaacaaagc ccgaaaggaa gctgagttgg ctgctgccac cgctgagcaa taactagcat
      541 aaccccttgg ggcctctaaa cgggtcttga ggggtttttt gctgaaagga ggaactatat
      601 ccggatatcc cgcaagaggc ccggcagtac cggcataacc aagcctatgc ctacagcatc
      661 cagggtgacg gtgccgagga tgacgatgag cgcattgtta gatttcatac acggtgcctg
      721 actgcgttag caatttaact gtgataaact accgcattaa agcttatcga tgataagctg
      781 tcaaacatga gaattcttga agacgaaagg gcctcgtgat acgcctattt ttataggtta
      841 atgtcatgat aataatggtt tcttagacgt
//
