LOCUS       sequence-86468-e         846 bp    DNA     linear   UNA 14-APR-2025
DEFINITION  expression of full length untagged human RanGAP1 in E.coli.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 846)
  AUTHORS   Werner A, Flotho A, Melchior F
  TITLE     The RanBP2/RanGAP1*SUMO1/Ubc9 complex is a multisubunit SUMO E3 
            ligase.
  JOURNAL   Mol Cell. 2012 May 11;46(3):287-98. doi: 
            10.1016/j.molcel.2012.02.017. Epub 2012 Mar 29.
  PUBMED    22464730
REFERENCE   2  (bases 1 to 846)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 14, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.molcel.2012.02.017"; journalName: "Mol Cell"; date: 
            "2012-05-11- 11"; volume: "46"; issue: "3"; pages: "287-98"
COMMENT     Sequence Label: pET11a-hRanGAP1
FEATURES             Location/Qualifiers
     source          1..846
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     52..71
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        52..70
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    71..95
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     RBS             110..132
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
ORIGIN
        1 ccggccacga tgcgtccggc gtagaggatc gagatctcga tcccgcgaaa ttaatacgac
       61 tcactatagg ggaattgtga gcggataaca attcccctct agaaataatt ttgtttaact
      121 ttaagaagga gatatacata tggcctcgga agacattgcc aagctggcag agacacttgc
      181 caagactcag gtggccgggg gacagctgag tttcaaaggc aagagcctca aactcaacac
      241 tgcagaagat gctaaagatg tgattaaaga gattgaagac tttgacagct tggaggctct
      301 gcgtctggaa ggcaacacag tgggcgtgga agccgccagg gtcatcgcca aggccttaga
      361 gaagaagtcg gagttgaagc gctgccactg gagtgacatg ttcacgggaa ggctgcggac
      421 cgagatccca ccagccctga tctcactagg ggaaggactc atcacagctg gggctcagct
      481 ggtggagctg gacttaagcg acaacgcatt cgggcccgac ggtgtgcaag gcttcgaggc
      541 cctgctcaag agctcagcct gcttcaccct gcaggaactc aagctcaaca actgtggcat
      601 gggcattggc ggcggcaaga tcctggctgc agctctgacc gaatgtcacc ggaaatccag
      661 tgcccaaggc aagcctctgg ccctgaaggt ctttgtggct ggcagaaacc gtctggagaa
      721 tgatggcgcc actgccttgg cagaagcttt tagggtcatc gggaccctgg aggaggtcca
      781 catgccacag aatgggatca accaccctgg catcactgcc ctggcccagg ctttcgctgt
      841 caaccc
//
