LOCUS       sequence-86590-e         923 bp    DNA     linear   UNA 15-APR-2025
DEFINITION  bacterial expression plasmid.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 923)
  TITLE     SGC_OxfordQ1Q2_2014
REFERENCE   2  (bases 1 to 923)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: STK3A-c042
FEATURES             Location/Qualifiers
     source          1..923
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             19..41
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             52..69
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             94..114
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
     CDS             330..347
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(400..418)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      414..461
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     rep_origin      498..878
                     /direction=RIGHT
                     /label=M13 ori
                     /note="M13 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(585..604)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     795..816
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
ORIGIN
        1 attccctcta gaaataattt tgtttaactt taagaaggag atatacatat gcaccatcat
       61 catcatcatt cttctggtgt agatctgggt accgagaacc tgtacttcca atccatggac
      121 tttgactttt tgaaaaatct aagtttagaa gaactacaga tgcggttaaa agcactggac
      181 cccatgatgg aacgggagat agaagaactt cgtcagagat acactgcgaa aagacagccc
      241 attctggatg cgatggatgc aaagtgacag taaaggtgga tacggatccg aattcgagct
      301 ccgtcgacaa gcttgcggcc gcactcgagc accaccacca ccaccactga gatccggctg
      361 ctaacaaagc ccgaaaggaa gctgagttgg ctgctgccac cgctgagcaa taactagcat
      421 aaccccttgg ggcctctaaa cgggtcttga ggggtttttt gctgaaagga ggaactatat
      481 ccggattggc gaatgggacg cgccctgtag cggcgcatta agcgcggcgg gtgtggtggt
      541 tacgcgcagc gtgaccgcta cacttgccag cgccctagcg cccgctcctt tcgctttctt
      601 cccttccttt ctcgccacgt tcgccggctt tccccgtcaa gctctaaatc gggggctccc
      661 tttagggttc cgatttagtg ctttacggca cctcgacccc aaaaaacttg attagggtga
      721 tggttcacgt agtgggccat cgccctgata gacggttttt cgccctttga cgttggagtc
      781 cacgttcttt aatagtggac tcttgttcca aactggaaca acactcaacc ctatctcggt
      841 ctattctttt gatttataag ggattttgcc gatttcggcc tattggttaa aaaatgagct
      901 gatttaacaa aaatttaacg cga
//
