LOCUS       sequence-86744-e        1050 bp    DNA     linear   UNA 14-APR-2025
DEFINITION  Localization: Endoplasmic Reticulum, Excitation: 558, Emission: 583.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1050)
  AUTHORS   Day RN, Davidson MW
  TITLE     The fluorescent protein palette: tools for cellular imaging.
  JOURNAL   Chem Soc Rev. 2009 Oct;38(10):2887-921. doi: 10.1039/b901966a. Epub 
            2009 Aug 4.
  PUBMED    19771335
REFERENCE   2  (bases 1 to 1050)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 14, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1039/b901966a"; 
            journalName: "Chem Soc Rev"; date: "2009-10"; volume: "38"; issue: 
            "10"; pages: "2887-921"
COMMENT     Sequence Label: DsRed2-ER-5
FEATURES             Location/Qualifiers
     source          1..1050
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        27..78
                     /label=CMVd2 promoter
                     /note="truncated human cytomegalovirus (CMV) immediate 
                     early promoter that yields moderately reduced expression 
                     relative to the full-length promoter"
     primer_bind     29..49
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     CDS             173..847
                     /codon_start=1
                     /product="improved tetrameric variant of DsRed fluorescent 
                     protein"
                     /label=DsRed2
                     /note="mammalian codon-optimized"
                     /translation="MASSENVITEFMRFKVRMEGTVNGHEFEIEGEGEGRPYEGHNTVK
                     LKVTKGGPLPFAWDILSPQFQYGSKVYVKHPADIPDYKKLSFPEGFKWERVMNFEDGGV
                     ATVTQDSSLQDGCFIYKVKFIGVNFPSDGPVMQKKTMGWEASTERLYPRDGVLKGETHK
                     ALKLKDGGHYLVEFKSIYMAKKPVQLPGYYYVDAKLDITSHNEDYTIVEQYERTEGRHH
                     LFL"
     primer_bind     complement(353..373)
                     /label=DsRed1-N
                     /note="DsRed1, reverse primer"
     primer_bind     765..788
                     /label=DsRed1-C
                     /note="DsRed1, forward primer"
     misc_feature    875..931
                     /label=MCS
                     /note="multiple cloning site"
ORIGIN
        1 aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt acggtgggag
       61 gtctatataa gcagagctgg tttagtgaac cgtcagatcc gctagcatgc tgctatccgt
      121 gccgttgctg ctcggcctcc tcggcctggc cgtcgccgac cggtcgcaca ccatggcctc
      181 ctccgagaac gtcatcaccg agttcatgcg cttcaaggtg cgcatggagg gcaccgtgaa
      241 cggccacgag ttcgagatcg agggcgaggg cgagggccgc ccctacgagg gccacaacac
      301 cgtgaagctg aaggtgacca agggcggccc cctgcccttc gcctgggaca tcctgtcccc
      361 ccagttccag tacggctcca aggtgtacgt gaagcacccc gccgacatcc ccgactacaa
      421 gaagctgtcc ttccccgagg gcttcaagtg ggagcgcgtg atgaacttcg aggacggcgg
      481 cgtggcgacc gtgacccagg actcctccct gcaggacggc tgcttcatct acaaggtgaa
      541 gttcatcggc gtgaacttcc cctccgacgg ccccgtgatg cagaagaaga ccatgggctg
      601 ggaggcctcc accgagcgcc tgtacccccg cgacggcgtg ctgaagggcg agacccacaa
      661 ggccctgaag ctgaaggacg gcggccacta cctggtggag ttcaagtcca tctacatggc
      721 caagaagccc gtgcagctgc ccggctacta ctacgtggac gccaagctgg acatcacctc
      781 ccacaacgag gactacacca tcgtggagca gtacgagcgc accgagggcc gccaccacct
      841 gttcctgaga tcgtacaaga aggacgagct gtaaagatct cgagctcaag cttcgaattc
      901 tgcagtcgac ggtaccgcgg gcccgggatc caccggatct agataactga tcataatcag
      961 ccataccaca tttgtagagg ttttacttgc tttaaaaaac ctcccacacc tccccctgaa
     1021 cctgaaacat aaaatgaatg caattgttgt
//
