LOCUS       sequence-86815-e        1980 bp    DNA     linear   UNA 14-APR-2025
DEFINITION  Localization: Mitochondria, Excitation: 559, Emission: 600.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1980)
  AUTHORS   Lam AJ, St-Pierre F, Gong Y, Marshall JD, Cranfill PJ, Baird MA, 
            McKeown MR, Wiedenmann J, Davidson MW, Schnitzer MJ, Tsien RY, Lin 
            MZ
  TITLE     Improving FRET dynamic range with bright green and red fluorescent 
            proteins.
  JOURNAL   Nat Methods. 2012 Sep 9. doi: 10.1038/nmeth.2171.
  PUBMED    22961245
REFERENCE   2  (bases 1 to 1980)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 14, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Methods. 2012 Sep 9. doi: 10.1038/nmeth.2171."
COMMENT     Sequence Label: mRuby2-PDHA1-N-10
FEATURES             Location/Qualifiers
     source          1..1980
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      1223..1232
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             1229..1939
                     /codon_start=1
                     /product="monomeric red fluorescent protein derived from 
                     mRuby, with improved photophysical properties (Lam et al., 
                     2012)"
                     /label=mRuby2
                     /translation="MVSKGEELIKENMRMKVVMEGSVNGHQFKCTGEGEGNPYMGTQTM
                     RIKVIEGGPLPFAFDILATSFMYGSRTFIKYPKGIPDFFKQSFPEGFTWERVTRYEDGG
                     VVTVMQDTSLEDGCLVYHVQVRGVNFPSNGPVMQKKTKGWEPNTEMMYPADGGLRGYTH
                     MALKVDGGGHLSCSFVTTYRSKKTVGNIKMPGIHAVDHRLERLEESDNEMFVVQREHAV
                     AKFAGLGGGMDELYK"
ORIGIN
        1 gaccgtcaga tccgctagca ccgccaccat gaggaagatg ctcgccgccg tctcccgcgt
       61 gctgtctggc gcttctcaga agccggcaag cagagtgctg gtagcatccc gtaattttgc
      121 aaatgatgct acatttgaaa ttaagaaatg tgaccttcac cggctggaag aaggccctcc
      181 tgtcacaaca gtgctcacca gggaggatgg gctcaaatac tacaggatga tgcagactgt
      241 acgccgaatg gagttgaaag cagatcagct gtataaacag aaaattattc gtggtttctg
      301 tcacttgtgt gatggtcagg aagcttgctg tgtgggcctg gaggccggca tcaaccccac
      361 agaccatctc atcacagcct accgggctca cggctttact ttcacccggg gcctttccgt
      421 ccgagaaatt ctcgcagagc ttacaggacg aaaaggaggt tgtgctaaag ggaaaggagg
      481 atcgatgcac atgtatgcca agaacttcta cgggggcaat ggcatcgtgg gagcgcaggt
      541 gcccctgggc gctgggattg ctctagcctg taagtataat ggaaaagatg aggtctgcct
      601 gactttatat ggcgatggtg ctgctaacca gggccagata ttcgaagctt acaacatggc
      661 agctttgtgg aaattacctt gtattttcat ctgtgagaat aatcgctatg gaatgggaac
      721 gtctgttgag agagcggcag ccagcactga ttactacaag agaggcgatt tcattcctgg
      781 gctgagagtg gatggaatgg atatcctgtg cgtccgagag gcaacaaggt ttgctgctgc
      841 ctattgtaga tctgggaagg ggcccatcct gatggagctg cagacttacc gttaccacgg
      901 acacagtatg agtgaccctg gagtcagtta ccgtacacga gaagaaattc aggaagtaag
      961 aagtaagagt gaccctatta tgcttctcaa ggacaggatg gtgaacagca atcttgccag
     1021 tgtggaagaa ctaaaggaaa ttgatgtgga agtgaggaag gagattgagg atgctgccca
     1081 gtttgccacg gccgatcctg agccaccttt ggaagagctg ggctaccaca tctactccag
     1141 cgacccacct tttgaagttc gtggtgccaa tcagtggatc aagtttaagt cagtcagtag
     1201 ctccggaggg gatccaccgg tcgccaccat ggtgtctaag ggcgaagagc tgatcaagga
     1261 aaatatgcgt atgaaggtgg tcatggaagg ttcggtcaac ggccaccaat tcaaatgcac
     1321 aggtgaagga gaaggcaatc cgtacatggg aactcaaacc atgaggatca aagtcatcga
     1381 gggaggaccc ctgccatttg cctttgacat tcttgccacg tcgttcatgt atggcagccg
     1441 tacttttatc aagtacccga aaggcattcc tgatttcttt aaacagtcct ttcctgaggg
     1501 ttttacttgg gaaagagtta cgagatacga agatggtgga gtcgtcaccg tcatgcagga
     1561 caccagcctt gaggatggct gtctcgttta ccacgtccaa gtcagagggg taaactttcc
     1621 ctccaatggt cccgtgatgc agaagaagac caagggttgg gagcctaata cagagatgat
     1681 gtatccagca gatggtggtc tgaggggata cactcatatg gcactgaaag ttgatggtgg
     1741 tggccatctg tcttgctctt tcgtaacaac ttacaggtca aaaaagaccg tcgggaacat
     1801 caagatgccc ggtatccatg ccgttgatca ccgcctggaa aggttagagg aaagtgacaa
     1861 tgaaatgttc gtagtacaac gcgaacacgc agttgccaag ttcgccgggc ttggtggtgg
     1921 gatggacgag ctgtacaagt aagcggccgc gactctagat cataatcagc cataccacat
//
