LOCUS       sequence-86816-e        1391 bp    DNA     linear   UNA 14-APR-2025
DEFINITION  Localization: Peroxisomes, Excitation: 559, Emission: 600.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1391)
  AUTHORS   Lam AJ, St-Pierre F, Gong Y, Marshall JD, Cranfill PJ, Baird MA, 
            McKeown MR, Wiedenmann J, Davidson MW, Schnitzer MJ, Tsien RY, Lin 
            MZ
  TITLE     Improving FRET dynamic range with bright green and red fluorescent 
            proteins.
  JOURNAL   Nat Methods. 2012 Sep 9. doi: 10.1038/nmeth.2171.
  PUBMED    22961245
REFERENCE   2  (bases 1 to 1391)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 14, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Methods. 2012 Sep 9. doi: 10.1038/nmeth.2171."
COMMENT     Sequence Label: mRuby2-PMP-N-10
FEATURES             Location/Qualifiers
     source          1..1391
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      28..37
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     regulatory      643..652
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             649..1359
                     /codon_start=1
                     /product="monomeric red fluorescent protein derived from 
                     mRuby, with improved photophysical properties (Lam et al., 
                     2012)"
                     /label=mRuby2
                     /translation="MVSKGEELIKENMRMKVVMEGSVNGHQFKCTGEGEGNPYMGTQTM
                     RIKVIEGGPLPFAFDILATSFMYGSRTFIKYPKGIPDFFKQSFPEGFTWERVTRYEDGG
                     VVTVMQDTSLEDGCLVYHVQVRGVNFPSNGPVMQKKTKGWEPNTEMMYPADGGLRGYTH
                     MALKVDGGGHLSCSFVTTYRSKKTVGNIKMPGIHAVDHRLERLEESDNEMFVVQREHAV
                     AKFAGLGGGMDELYK"
ORIGIN
        1 tccgctagcg ctaccggact cagatctgcc accatggcgc cggccgcgtc caggctgcgg
       61 gccgaagccg ggctcggggc gctgccgcgg cgggcgctcg cccagtactt gctcttcctg
      121 cggctctacc cggtgctcac caaggcggcc accagtggca ttttgtcagc acttgggaac
      181 ttcctggccc agatgattga gaagaagcgg aaaaaagaaa actctagaag tctggatgtc
      241 ggtgggcctc tgagatatgc cgtttacggg ttcttcttca cagggccgct gagtcacttc
      301 ttctacttct tcatggaaca ttggatccct cctgaggtcc ccctggcagg gctcaggagg
      361 cttctcctgg accgcctcgt ctttgcaccg gccttcctca tgttgttctt cctcatcatg
      421 aactttctgg aggggaaaga cgcctcagcc ttcgccgcca agatgagggg gggcttctgg
      481 ccggcgctga ggatgaactg gcgggtgtgg acgccactac agttcatcaa catcaactac
      541 gtccctctga agttccgggt gctcttcgcc aacctggcag ctctgttctg gtatgcctac
      601 ctggcctcct tggggaagag cggctccggt agcggaccgg tcgccaccat ggtgtctaag
      661 ggcgaagagc tgatcaagga aaatatgcgt atgaaggtgg tcatggaagg ttcggtcaac
      721 ggccaccaat tcaaatgcac aggtgaagga gaaggcaatc cgtacatggg aactcaaacc
      781 atgaggatca aagtcatcga gggaggaccc ctgccatttg cctttgacat tcttgccacg
      841 tcgttcatgt atggcagccg tacttttatc aagtacccga aaggcattcc tgatttcttt
      901 aaacagtcct ttcctgaggg ttttacttgg gaaagagtta cgagatacga agatggtgga
      961 gtcgtcaccg tcatgcagga caccagcctt gaggatggct gtctcgttta ccacgtccaa
     1021 gtcagagggg taaactttcc ctccaatggt cccgtgatgc agaagaagac caagggttgg
     1081 gagcctaata cagagatgat gtatccagca gatggtggtc tgaggggata cactcatatg
     1141 gcactgaaag ttgatggtgg tggccatctg tcttgctctt tcgtaacaac ttacaggtca
     1201 aaaaagaccg tcgggaacat caagatgccc ggtatccatg ccgttgatca ccgcctggaa
     1261 aggttagagg aaagtgacaa tgaaatgttc gtagtacaac gcgaacacgc agttgccaag
     1321 ttcgccgggc ttggtggtgg gatggacgag ctgtacaagt aagcggccgc gactctagat
     1381 cataatcagc c
//
