LOCUS       sequence-86920-e        1930 bp    DNA     linear   UNA 15-APR-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1930)
  TITLE     Davidson Red fluorescent proteins
REFERENCE   2  (bases 1 to 1930)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: mPlum-CT6-Actin-7
FEATURES             Location/Qualifiers
     source          1..1930
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             35..730
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mTangerine
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEVIKEFMRFKEHMEGSVNGHEFEIEGEGEGRPYEGTQTA
                     RLKVTKGGPLPFAWDILSPQIMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNFEDGG
                     VVTVTQDSSLQDGEFIYKVKVRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALKGEMK
                     MRLRLKDGGHYDAEVKTTYMAKKPVQLPGAYKTDIKLDITSHNEDYTIVEQYERAEGRH
                     STGAMDELYK"
     primer_bind     436..455
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     primer_bind     630..653
                     /label=DsRed1-C
                     /note="DsRed1, forward primer"
ORIGIN
        1 gacgtcagat ccgctagcgc taccggtggc taccatggtg agcaagggcg aggaggtcat
       61 caaggagttc atgcgcttca aggagcacat ggagggctcc gtgaacggcc acgagttcga
      121 gatcgagggc gagggcgagg gccgccccta cgagggcacc cagaccgcca ggctgaaggt
      181 gaccaagggt ggccccctgc ccttcgcctg ggacatcctg tcccctcaga tcatgtacgg
      241 ctccaaggcc tacgtgaagc accccgccga catccccgac tacttgaagc tgtccttccc
      301 cgagggcttc aagtgggagc gcgtgatgaa cttcgaggac ggcggcgtgg tgaccgtgac
      361 ccaggactcc tccctgcagg acggcgagtt catctacaag gtgaaggtgc gcggcaccaa
      421 cttcccctcc gacggccccg taatgcagaa gaagaccatg ggctgggagg cctcctccga
      481 gcggatgtac cccgaggacg gcgccctgaa gggcgagatg aagatgaggc tgaggctgaa
      541 ggacggcggc cactacgacg ccgaggtcaa gaccacctac atggccaaga agcccgtgca
      601 gctgcccggc gcctacaaga ccgacatcaa gctggacatc acctcccaca acgaggacta
      661 caccatcgtg gaacagtacg agcgcgccga gggccgccac tccaccggcg ccatggacga
      721 gctgtacaag tccggactca gatctcgagc tatggatgat gatatcgccg cgctcgtcgt
      781 cgacaacggc tccggcatgt gcaaggccgg cttcgcgggc gacgatgccc cccgggccgt
      841 cttcccctcc atcgtggggc gccccaggca ccagggcgtg atggtgggca tgggtcagaa
      901 ggattcctat gtgggcgacg aggcccagag caagagaggc atcctcaccc tgaagtaccc
      961 catcgagcac ggcatcgtca ccaactggga cgacatggag aaaatctggc accacacctt
     1021 ctacaatgag ctgcgtgtgg ctcccgagga gcaccccgtg ctgctgaccg aggcccccct
     1081 gaaccccaag gccaaccgcg agaagatgac ccagatcatg tttgagacct tcaacacccc
     1141 agccatgtac gttgctatcc aggctgtgct atccctgtac gcctctggcc gtaccactgg
     1201 catcgtgatg gactccggtg acggggtcac ccacactgtg cccatctacg aggggtatgc
     1261 cctcccccat gccatcctgc gtctggacct ggctggccgg gacctgactg actacctcat
     1321 gaagatcctc accgagcgcg gctacagctt caccaccacg gccgagcggg aaatcgtgcg
     1381 tgacattaag gagaagctgt gctacgtcgc cctggacttc gagcaagaga tggccacggc
     1441 tgcttccagc tcctccctgg agaagagcta cgagctgcct gacggccagg tcatcaccat
     1501 tggcaatgag cggttccgct gccctgaggc actcttccag ccttccttcc tgggcatgga
     1561 gtcctgtggc atccacgaaa ctaccttcaa ctccatcatg aagtgtgacg tggacatccg
     1621 caaagacctg tacgccaaca cagtgctgtc tggcggcacc accatgtacc ctggcattgc
     1681 cgacaggatg cagaaggaga tcactgccct ggcacccagc acaatgaaga tcaagatcat
     1741 tgctcctcct gagcgcaagt actccgtgtg gatcggcggc tccatcctgg cctcgctgtc
     1801 caccttccag cagatgtgga tcagcaagca ggagtatgac gagtccggcc cctccatcgt
     1861 ccaccgcaaa tgcttctagg gatccaccgg atctagataa ctgatcataa tcagccatac
     1921 cacattgaga
//
