LOCUS       sequence-87019-e        1511 bp    DNA     linear   UNA 15-APR-2025
DEFINITION  Localization: Membrane, Excitation: 580, Emission: 608.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1511)
  AUTHORS   Shemiakina II, Ermakova GV, Cranfill PJ, Baird MA, Evans RA, 
            Souslova EA, Staroverov DB, Gorokhovatsky AY, Putintseva EV, 
            Gorodnicheva TV, Chepurnykh TV, Strukova L, Lukyanov S, Zaraisky AG,
            Davidson MW, Chudakov DM, Shcherbo D
  TITLE     A monomeric red fluorescent protein with low cytotoxicity.
  JOURNAL   Nat Commun. 2012;3:1204. doi: 10.1038/ncomms2208.
  PUBMED    23149748
REFERENCE   2  (bases 1 to 1511)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2012"; volume: "3:1204. doi"; pages: " 
            10.1038/ncomms2208"
COMMENT     Sequence Label: FusionRed-Dectin1A-N-10
FEATURES             Location/Qualifiers
     source          1..1511
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      13..22
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     regulatory      784..793
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             790..1488
                     /codon_start=1
                     /product="monomeric red fluorescent protein optimized for 
                     use as a fusion tag (Shemiakina et al., 2012)"
                     /label=FusionRed
                     /note="mammalian codon-optimized"
                     /translation="MVSELIKENMPMKLYMEGTVNNHHFKCTSEGEGKPYEGTQTMRIK
                     VVEGGPLPFAFDILATSFMYGSRTFIKHPPGIPDFFKQSFPEGFTWERVTTYEDGGVLT
                     ATQDTSLQDGCLIYNVKVRGVNFPANGPVMQKKTLGWEASTETMYPADGGLEGACDMAL
                     KLVGGGHLICNLETTYRSKKPATNLKMPGVYNVDHRLERIKEADDETYVEQHEVAVARY
                     STGGAGDGGK"
ORIGIN
        1 agatccgcta gcgccaccat ggaatatcat cctgatttag aaaatttgga tgaagatgga
       61 tatactcaat tacacttcga ctctcaaagc aataccagga tagctgttgt ttcagagaaa
      121 ggatcgtgtg ctgcatctcc tccttggcgc ctcattgctg taattttggg aatcctatgc
      181 ttggtaatac tggtgatagc tgtggtcctg ggtaccatgg ctatttggag atccaattca
      241 ggaagcaaca cattggagaa tggctacttt ctatcaagaa ataaagagaa ccacagtcaa
      301 cccacacaat catctttaga agacagtgtg actcctacca aagctgtcaa aaccacaggg
      361 gttctttcca gcccttgtcc tcctaattgg attatatatg agaagagctg ttatctattc
      421 agcatgtcac taaattcctg ggatggaagt aaaagacaat gctggcaact gggctctaat
      481 ctcctaaaga tagacagctc aaatgaattg ggatttatag taaaacaagt gtcttcccaa
      541 cctgataatt cattttggat aggcctttct cggccccaga ctgaagtacc atggctctgg
      601 gaggatggat caacattctc ttctaactta tttcagatca gaaccacagc tacccaagaa
      661 aacccatctc caaattgtgt atggattcac gtgtcagtca tttatgacca actgtgtagt
      721 gtgccctcat atagtatttg tgagaagaag ttttcaatga gctccggagg ggatccaccg
      781 gtcgccacca tggtgagcga gctgattaag gagaacatgc ccatgaagct gtacatggag
      841 ggcaccgtga acaaccacca cttcaagtgc acatccgagg gcgaaggcaa gccctacgag
      901 ggcacccaga ccatgagaat caaggtcgtc gagggcggcc ctctcccctt cgccttcgac
      961 atcctggcta ccagcttcat gtacggcagc agaaccttca tcaagcaccc tccgggcatc
     1021 cccgacttct ttaagcagtc cttccctgag ggcttcacat gggagagagt caccacatac
     1081 gaagacgggg gcgtgctgac cgctacccag gacaccagcc tccaggacgg ctgcctcatc
     1141 tacaacgtca aggttagagg ggtgaacttc ccagccaacg gccctgtgat gcagaagaaa
     1201 acactcggct gggaggcctc caccgagacg atgtaccccg ctgacggcgg cctggaaggc
     1261 gcatgtgaca tggccctgaa gctcgtgggc gggggccacc tgatctgcaa ccttgagacc
     1321 acatacagat ccaagaaacc cgctacgaac ctcaagatgc ccggcgtcta caacgtggac
     1381 cacagactgg aaagaatcaa ggaggccgac gatgagacct acgtcgagca gcacgaggtg
     1441 gctgtggcca gatactctac tggtggcgct ggtgatggag gtaaatgagc ggccgcgact
     1501 ctagatcata a
//
