LOCUS       sequence-87185-e         897 bp    DNA     linear   UNA 14-APR-2025
DEFINITION  Localization: Endoplasmic Reticulum, Excitation: 399, Emission: 511.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 897)
  TITLE     Davidson Green fluorescent proteins
REFERENCE   2  (bases 1 to 897)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 14, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: mT-Sapphire-CytERM-N-17
FEATURES             Location/Qualifiers
     source          1..897
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      168..177
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(174..176,177..179,180..890)
                     /codon_start=1
                     /product="strongly enhanced green fluorescent protein 
                     (Kremers et al., 2007)"
                     /label=SGFP2
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTFSYGVMVFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNFNSHNVYIMADKQKNGIK
                     ANFKIRHNIEDGGVQLADHYQQNTPIGDGPVLLPDNHYLSIQSKLSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(219..240)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(480..499)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     827..848
                     /label=EGFP-C
                     /note="EGFP, forward primer"
ORIGIN
        1 ttgcagcttc cagttgtcag gaagagaagg ctcccatgga tcctgtggtg gtgctggggc
       61 tctgtctctc ctgtttgctt ctcctttcac tctggaaaca gagctatggg ggagggaagc
      121 ttcgaattct gcagtcgacg gtaccgcggg cccgggatcc accggtcgcc accatggtga
      181 gcaagggcga ggagctgttc accggggtgg tgcccatcct ggtcgagctg gacggcgacg
      241 taaacggcca caagttcagc gtgtccggcg agggcgaggg cgatgccacc tacggcaagc
      301 tgaccctgaa gttcatctgc accaccggca agctgcccgt gccctggccc accctcgtga
      361 ccaccttctc ctacggcgtg atggtgttcg cccgctaccc cgaccacatg aagcagcacg
      421 acttcttcaa gtccgccatg cccgaaggct acgtccagga gcgcaccatc ttcttcaagg
      481 acgacggcaa ctacaagacc cgcgccgagg tgaagttcga gggcgacacc ctggtgaacc
      541 gcatcgagct gaagggcatc gacttcaagg aggacggcaa catcctgggg cacaagctgg
      601 agtacaactt caacagccac aacgtctata tcatggccga caagcagaag aacggcatca
      661 aggccaactt caagatccgc cacaacatcg aggacggcgg cgtgcagctc gccgaccact
      721 accagcagaa cacccccatc ggcgacggcc ccgtgctgct gcccgacaac cactacctga
      781 gcatccagtc caagctgagc aaagacccca acgagaagcg cgatcacatg gtcctgctgg
      841 agttcgtgac cgccgccggg atcactctgg gcatggacga gctgtacaag taagcgg
//
