LOCUS       sequence-87293-e        2225 bp    DNA     linear   UNA 15-APR-2025
DEFINITION  Localization: Microtubules, Excitation: 530, Emission: 541.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2225)
  AUTHORS   Hoi H, Howe ES, Ding Y, Zhang W, Baird MA, Sell BR, Allen JR, 
            Davidson MW, Campbell RE
  TITLE     An engineered monomeric Zoanthus sp. yellow fluorescent protein.
  JOURNAL   Chem Biol. 2013 Oct 24;20(10):1296-304. doi: 
            10.1016/j.chembiol.2013.08.008. Epub 2013 Oct 3.
  PUBMED    24094838
REFERENCE   2  (bases 1 to 2225)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.chembiol.2013.08.008"; journalName: "Chem Biol"; date: 
            "2013-10-24- 24"; volume: "20"; issue: "10"; pages: "1296-304"
COMMENT     Sequence Label: mPapaya-Tubulin-C-18
FEATURES             Location/Qualifiers
     source          1..2225
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      40..49
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             46..756
                     /codon_start=1
                     /product="monomeric yellow fluorescent protein derived from
                     the Zoanthus fluorescent protein zFP538"
                     /label=mPapaya1
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEGQSKHGLKEEMTVKYHMEGCVNGHKFVITGEGIGNPFKG
                     KQTANLCVIEGGPLPFSEDILSPGFKYGDRIFTEYPQDIVDYFKNSCPAGYTWERSFLF
                     EDGAVCRCNVDITVSEKENCIYHKSIFRGVNFPADGPVMKKMTTNWEASTEKIVPVPKQ
                     GILKGKVKMYLLLKDGGRYHCQFDTVYKAKSVPSKMPEWHFIQHKLLREDRSDAKNQKW
                     QLTEHAIAGMDELYK"
ORIGIN
        1 cccgtgctgg tgacgtcaga tccgctagcg ctaccggtcg ccaccatggt gagcaagggc
       61 gaggggcaat cgaagcatgg gctaaaggag gagatgacgg tgaagtacca catggaaggt
      121 tgcgttaatg gtcacaaatt tgtcattact ggagagggca ttggaaaccc ttttaagggt
      181 aagcaaactg caaatttgtg tgtgatagaa ggaggcccgc tgccgttctc ggaggacatt
      241 ttaagtccgg gttttaaata tggtgaccgg attttcacag agtacccaca ggatattgta
      301 gattacttca agaactcatg tccagcgggc tatacgtggg aaaggagctt cctctttgag
      361 gacggagcgg tctgtcgatg caacgtggac ataacagtct ctgaaaagga gaactgcatc
      421 tatcacaaaa gtatcttcag aggggtgaat tttcccgccg acggccccgt aatgaagaaa
      481 atgaccacta attgggaagc tagtaccgag aaaattgtgc ctgttccaaa gcaagggata
      541 ttaaaaggaa aggtcaaaat gtacctattg ctgaaggatg gcggtcgtta tcattgccag
      601 tttgatacgg tatataaagc taaatcagtg ccctccaaga tgccagaatg gcattttata
      661 cagcataagc tccttaggga ggaccgctct gatgctaaaa accagaaatg gcaattaaca
      721 gaacatgcaa tcgcaggcat ggacgagctg tacaagtccg gactcagatc tggcagcggt
      781 ggaggcagcg catccggcgg aagcggaagc gtgcgtgagt gcatctccat ccacgttggc
      841 caggctggtg tccagattgg caatgcctgc tgggagctct actgcctgga acacggcatc
      901 cagcccgatg gccagatgcc aagtgacaag accattgggg gaggagatga ctccttcaac
      961 accttcttca gtgagacggg cgctggcaag cacgtgcccc gggctgtgtt tgtagacttg
     1021 gaacccacag tcattgatga agttcgcact ggcacctacc gccagctctt ccaccctgag
     1081 cagctcatca caggcaagga agatgctgcc aataactatg cccgagggca ctacaccatt
     1141 ggcaaggaga tcattgacct tgtgttggac cgaattcgca agctggctga ccagtgcacc
     1201 ggtcttcagg gcttcttggt tttccacagc tttggtgggg gaactggttc tgggttcacc
     1261 tccctgctca tggaacgtct ctcagttgat tatggcaaga agtccaagct ggagttctcc
     1321 atttacccag caccccaggt ttccacagct gtagttgagc cctacaactc catcctcacc
     1381 acccacacca ccctggagca ctctgattgt gccttcatgg tagacaatga ggccatctat
     1441 gacatctgtc gtagaaacct cgatatcgag cgcccaacct acactaacct taaccgcctt
     1501 attagccaga ttgtgtcctc catcactgct tccctgagat ttgatggagc cctgaatgtt
     1561 gacctgacag aattccagac caacctggtg ccctaccccc gcatccactt ccctctggcc
     1621 acatatgccc ctgtcatctc tgctgagaaa gcctaccatg aacagctttc tgtagcagag
     1681 atcaccaatg cttgctttga gccagccaac cagatggtga aatgtgaccc tcgccatggt
     1741 aaatacatgg cttgctgcct gttgtaccgt ggtgacgtgg ttcccaaaga tgtcaatgct
     1801 gccattgcca ccatcaaaac caagcgcagc atccagtttg tggattggtg ccccactggc
     1861 ttcaaggttg gcatcaacta ccagcctccc actgtggtgc ctggtggaga cctggccaag
     1921 gtacagagag ctgtgtgcat gctgagcaac accacagcca ttgctgaggc ctgggctcgc
     1981 ctggaccaca agtttgacct gatgtatgcc aagcgtgcct ttgttcactg gtacgtgggt
     2041 gaggggatgg aggaaggcga gttttcagag gcccgtgaag atatggctgc ccttgagaag
     2101 gattatgagg aggttggtgt ggattctgtt gaaggagagg gtgaggaaga aggagaggaa
     2161 tactaaggat ccaccggatc tagataactg atcataatca gccataccac attgtagaag
     2221 aagcc
//
