LOCUS       sequence-87363-e        1669 bp    DNA     linear   UNA 15-APR-2025
DEFINITION  Localization: Nucleus, Excitation: 488, Emission: 507.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1669)
  TITLE     Davidson Green fluorescent proteins
REFERENCE   2  (bases 1 to 1669)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: mEGFP-Pit1-6
FEATURES             Location/Qualifiers
     source          1..1669
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      904..913
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(910..912,913..915,916..1629)
                     /codon_start=1
                     /product="enhanced GFP with monomerizing A206K mutation 
                     (Zacharias et al., 2002)"
                     /label=mEGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(955..976)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(1216..1235)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     1563..1584
                     /label=EGFP-C
                     /note="EGFP, forward primer"
ORIGIN
        1 gatccgctag ccgccaccat gagttgccaa cctttcacct cggctgatac ctttatacct
       61 ctgaattctg acgcttctgc tgccctgcct ctgagaatgc accacagtgc cgctgagggt
      121 ctcccagcct ccaaccacgc caccaacgtg atgtccacag cgacaggact tcattattct
      181 gtgccttcct gtcattatgg aaaccagcca tccacctacg gagtgatggc aggcacttta
      241 accccttgtc tttacaagtt tccagaccac accctgagtc atgggtttcc tcccctgcac
      301 caacctctgc tggcggagga cccaacagcc tctgaattta agcaggaact caggcggaaa
      361 agtaaattgg tggaagagcc aatagacatg gactccccgg aaatccgaga actggagcag
      421 tttgccaacg aatttaaagt gagaagaatt aagttaggat acacccagac aaacgtgggc
      481 gaagctctgg ccgctgtcca cggctccgaa ttcagtcaaa caaccatctg ccgatttgaa
      541 aacttgcagc tcagtttcaa aaatgcttgc aaactgaaag caattttatc caagtggctg
      601 gaggaagctg agcaggtcgg agctttgtac aatgaaaaag tgggagcaaa cgaaaggaag
      661 aggaaacgga ggacaactat cagtatcgcc gctaaggatg ctttggagag acactttgga
      721 gagcacagca aaccttcttc gcaggagatc atgcggatgg ctgaagaatt gaatctcgag
      781 aaagaagtag taagagtgtg gttttgcaac cgaaggcaga gagaaaaacg ggtgaaaacg
      841 agtctcaatc aaagtctgtt ctctatttca aaggagcatc ttgagtgcag agatccaccg
      901 gtcgccacca tggtgagcaa gggcgaggag ctgttcaccg gggtggtgcc catcctggtc
      961 gagctggacg gcgacgtaaa cggccacaag ttcagcgtgt ccggcgaggg cgagggcgat
     1021 gccacctacg gcaagctgac cctgaagttc atctgcacca ccggcaagct gcccgtgccc
     1081 tggcccaccc tcgtgaccac cctgacctac ggcgtgcagt gcttcagccg ctaccccgac
     1141 cacatgaagc agcacgactt cttcaagtcc gccatgcccg aaggctacgt ccaggagcgc
     1201 accatcttct tcaaggacga cggcaactac aagacccgcg ccgaggtgaa gttcgagggc
     1261 gacaccctgg tgaaccgcat cgagctgaag ggcatcgact tcaaggagga cggcaacatc
     1321 ctggggcaca agctggagta caactacaac agccacaacg tctatatcat ggccgacaag
     1381 cagaagaacg gcatcaaggt gaacttcaag atccgccaca acatcgagga cggcagcgtg
     1441 cagctcgccg accactacca gcagaacacc cccatcggcg acggccccgt gctgctgccc
     1501 gacaaccact acctgagcac ccagtccaag ctgagcaaag accccaacga gaagcgcgat
     1561 cacatggtcc tgctggagtt cgtgaccgcc gccgggatca ctctcggcat ggacgagctg
     1621 tacaagtaaa gcggccgcga ctctagatca taatcagcca taccacatt
//
